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PIGP (NM_153682) Human Untagged Clone

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SKU
SC110438
PIGP (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PIGP
Synonyms DCRC; DCRC-S; DEE55; DSCR5; DSRC; EIEE55; PIG-P
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC110438 sequence for NM_153682 edited (data generated by NextGen Sequencing)
ATGGTGGAAAATTCACCGTCGCCATTGCCAGAAAGAGCGATTTATGGCTTTGTTCTTTTC
TTAAGCTCCCAATTTGGCTTCATACTTTACCTCGTGTGGGCCTTTATTCCTGAATCTTGG
CTAAACTCTTTAGGTTTAACCTATTGGCCTCAAAAATATTGGGCAGTTGCATTACCTGTC
TACCTCCTTATTGCTATAGTAATTGGCTACGTGCTCTTGTTTGGGATTAACATGATGAGT
ACCTCTCCACTCGACTCCATCCATACAATCACAGATAACTATGCAAAAAATCAACAGCAG
AAGAAATACCAAGAGGAGGCCATTCCAGCCTTAAGAGATATTTCTATTAGTGAAGTAAAC
CAAATGTTCTTTCTTGCAGCCAAAGAACTTTACACCAAAAACTGA

Clone variation with respect to NM_153682.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_153682 unedited
CAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACCAGGGCGCTTGCG
CGGAGAACTCAGCGCTGAGATTGTCTAAACCCCAGAAAAATGGTGGAAAATTCACCGTCG
CCATTGCCAGAAAGAGCGATTTATGGCTTTGTTCTTTTCTTAAGCTCCCAATTTGGCTTC
ATACTTTACCTCGTGTGGGCCTTTATTCCTGAATCTTGGCTAAACTCTTTAGGTTTAACC
TATTGGCCTCAAAAATATTGGGCAGTTGCATTACCTGTCTACCTCCTTATTGCTATAGTA
ATTGGCTACGTGCTCTTGTTTGGGATTAACATGATGAGTACCTCTCCACTCGACTCCATC
CATACAATCACAGATAACTATGCAAAAAATCAACAGCAGAAGAAATACCAAGAGGAGGCC
ATTCCAGCCTTAAGAGATATTTCTATTAGTGAAGTAAACCAAATGTTCTTTCTTGCAGCC
AAAGAACTTTACACCAAAAACTGAACTGTGTGTAACCATAGTAACACCAAGCACGTATTT
ATTTATAAGTTTTTGCCATTATAATTTTGACCATAAATTAATTTGACCATCTCTCTTATT
AATAGAGAAGTAAAAAATGTAAGTTGACCTTCTCTTAGATTATGTTCAATGAATATTGTA
AATGTTCAAGTATTGTTAATGAATAGAATAAATACAATATTGCATTCCCATAAAAAAAAA
AAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCG
GGTGGCATCCCTGTGACCCCTNCCCAGTGCCTCTCCTGGCCCCTGAAGTTGCCACTCCAG
TGCCCACCAGCCTTTGTCTAATAAAATAAAGTGCATCATTTTCTGACTAGGTGTCCTCTT
ATATATGGGTANNGGGGGGGGNNNNTNNNNNCAAAAGGGACAATTTGGAAACACACCTGA
GGGCCTGGGGGGCTATGGGGACCAACTGGATGCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_153682 unedited
ATGGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATGGGAATGCAAT
ATTGTATTTATTCTATTCATTAACAATACTTGAACATTTACAATATTCATTGAACATAAT
CTAAGAGAAGGTCAACTTACATTTTTTACTTCTCTATTAATAAGAGAGATGGTCAAATTA
ATTTATGGTCAAAATTATAATGGCAAAAACTTATAAATAAATACGTGCTTGGTGTTACTA
TGGTTACACACAGTTCAGTTTTTGGTGTAAAGTTCTTTGGCTGCAAGAAAGAACATTTGG
TTTACTTCACTAATAGAAATATCTCTTAAGGCTGGAATGGCCTCCTCTTGGTATTTCTTC
TGCTGTTGATTTTTTGCATAGTTATCTGTGATTGTATGGATGGAGTCGAGTGGAGAGGTA
CTCATCATGTTAATCCCAAACAAGAGCACGTAGCCAATTACTATAGCAATAAGGAGGTAG
ACAGGTAATGCAACTGCCCAATATTTTTGAGGCCAATAGGTTAAACCTAAAGAGTTTAGC
CAAGATTCAGGAATAAAGGCCCACACGAGGTAAAGTATGAAGCCAAATTGGGAGCTTAAG
AAAAGAACAAAGCCATAAATCGCTCTTTCTGGCAATGGCGACGGTGAATTTTCCACCATT
TTTCCTGGGGCTTTAGACAATCTCAGCGCTGAGTTCTCCGCGCAAGCGCCCTGGTGCCGA
ATTCGCGGCCCGCCTATAGTGAGTCGTATACANAATTCTGACGGTTCACTAAACGAGCTC
TGCTTATATANGACCTCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGGGG
TTATTACGACATTTTGGAAAGTCCCCGTGATTTTGTTGCACAACAACTCCCATGACGTCA
AGGGGTGAGACTTGGAAACCCCGGAGTAAACGCTTCCACGCATTGTGACTGGCAACCGCT
TACCTGGAAAGCGAGACAAACGTA
Restriction Sites NotI-NotI |
ACCN NM_153682
Insert Size 680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153682.1, NP_710149.1
RefSeq Size 727 bp
RefSeq ORF 405 bp
Locus ID 51227
UniProt ID P57054
Cytogenetics 21q22.13
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
Summary This gene encodes an enzyme involved in the first step of glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. The encoded protein is a component of the GPI-N-acetylglucosaminyltransferase complex that catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). This gene is located in the Down Syndrome critical region on chromosome 21 and is a candidate for the pathogenesis of Down syndrome. This gene has multiple pseudogenes and is a member of the phosphatidylinositol glycan anchor biosynthesis gene family. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Feb 2016
Transcript Variant: This variant (2) contains a distinct 5' UTR and initiates translation from a downstream start codon, compared to variant 1. The encoded protein (isoform 2) has a shorter N-terminus compared to isoform 1. Variants 2 and 5 encode the same protein (isoform 2).
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Documents
Resources
"NM_153682" in other vectors (5)
SKU Description Size Price
RC203040 PIGP (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2 10 ug
$150.00
RC203040L3 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC203040L4 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2, mGFP tagged 10 ug
$450.00
RG203040 PIGP (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2 10 ug
$489.00
SC320893 PIGP (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2 10 ug
$150.00

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