AGMAT (NM_024758) Human Tagged ORF Clone

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SKU
RC202990
AGMAT (Myc-DDK-tagged)-Human agmatine ureohydrolase (agmatinase) (AGMAT)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AGMAT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202990 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGGCTGCTGGCGTCCGGGTGCGCCCGGGGCCCGGGGCCCGGCGTGGGCGCGCGTCCTGCCGCAG
GGCTCTTTCATCCGGGGCGCCGCCAGAGCCGCCAGGCTTCCGATGCGCCCCGGAACCAGCCCCCCAGCCC
CGAGTTCGTGGCCCGGCCGGTGGGCGTCTGCTCCATGATGCGCCTGCCGGTGCAGACCTCCCCCGAGGGG
CTGGACGCTGCCTTCATCGGGGTGCCCCTGGATACTGGGACCTCCAACCGGCCTGGGGCGAGATTCGGAC
CTCGCCGCATCCGGGAAGAATCAGTGATGCTTCGGACAGTCAATCCTAGCACGGGGGCCCTCCCCTTCCA
GTCCCTCATGGTTGCAGACCTAGGCGATGTGAATGTCAATCTTTACAACCTTCAGGACAGCTGCCGGCGA
ATTCAAGAGGCCTATGAGAAAATTGTAGCAGCTGGCTGTATTCCTCTGACCTTGGGTGGAGATCACACAA
TCACATATCCCATATTGCAAGCGATGGCAAAAAAGCATGGCCCAGTGGGGCTGCTGCACGTGGATGCGCA
CACGGACACGACCGACAAGGCCCTAGGAGAGAAGCTCTACCACGGGGCGCCCTTCCGCCGGTGTGTGGAT
GAGGGTCTCCTGGACTGTAAGCGTGTGGTGCAGATTGGCATCCGGGGCTCTTCCACGACCTTGGATCCCT
ACAGATACAACCGGAGCCAGGGCTTCCGGGTAGTCCTGGCTGAAGACTGCTGGATGAAGTCGCTGGTTCC
TCTGATGGGGGAAGTCAGGCAGCAGATGGGAGGCAAACCCATTTATATCAGCTTTGATATTGACGCTCTG
GATCCTGCCTATGCGCCAGGGACAGGGACACCTGAAATTGCTGGTCTCACTCCTAGTCAGGCTCTGGAGA
TCATCAGGGGTTGTCAAGGCCTGAACGTGATGGGCTGTGATCTTGTCGAAGTTTCACCACCGTATGATCT
TTCTGGGAACACAGCCCTGCTGGCGGCTAACCTGCTGTTTGAGATGCTATGTGCTCTCCCCAAAGTGACA
ACCGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202990 protein sequence
Red=Cloning site Green=Tags(s)

MLRLLASGCARGPGPGVGARPAAGLFHPGRRQSRQASDAPRNQPPSPEFVARPVGVCSMMRLPVQTSPEG
LDAAFIGVPLDTGTSNRPGARFGPRRIREESVMLRTVNPSTGALPFQSLMVADLGDVNVNLYNLQDSCRR
IQEAYEKIVAAGCIPLTLGGDHTITYPILQAMAKKHGPVGLLHVDAHTDTTDKALGEKLYHGAPFRRCVD
EGLLDCKRVVQIGIRGSSTTLDPYRYNRSQGFRVVLAEDCWMKSLVPLMGEVRQQMGGKPIYISFDIDAL
DPAYAPGTGTPEIAGLTPSQALEIIRGCQGLNVMGCDLVEVSPPYDLSGNTALLAANLLFEMLCALPKVT
TV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024758
ORF Size 1056 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024758.5
RefSeq Size 3154 bp
RefSeq ORF 1059 bp
Locus ID 79814
UniProt ID Q9BSE5
Cytogenetics 1p36.21
Domains arginase
Protein Pathways Arginine and proline metabolism, Metabolic pathways
MW 37.8 kDa
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Documents
Resources
"NM_024758" in other vectors (4)
SKU Description Size Price
RC202990L3 Lenti ORF clone of Human agmatine ureohydrolase (agmatinase) (AGMAT), Myc-DDK-tagged 10 ug
$757.00
RC202990L4 Lenti ORF clone of Human agmatine ureohydrolase (agmatinase) (AGMAT), mGFP tagged 10 ug
$757.00
RG202990 AGMAT (tGFP-tagged) - Human agmatine ureohydrolase (agmatinase) (AGMAT) 10 ug
$489.00 $657.00
SC320162 AGMAT (untagged)-Human agmatine ureohydrolase (agmatinase) (AGMAT) 10 ug
$457.00

Plasmid Map for RC202990

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Cloning scheme for RC202990

Circular map for RC202990

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