PYROXD2 (NM_032709) Human Tagged ORF Clone

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SKU
RC202983
PYROXD2 (Myc-DDK-tagged)-Human pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2)
  $811.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PYROXD2
Synonyms C10orf33; FP3420; YUEF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202983 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGCTGCAAGTGGCCGAGGTCTCTGCAAGGCTGTGGCCGCCTCTCCCTTCCCGGCGTGGAGACGAGATA
ACACGGAAGCCAGGGGAGGTCTGAAGCCTGAGTATGATGCGGTGGTGATAGGAGCAGGACACAACGGACT
GGTGGCTGCAGCGTACCTGCAGAGACTGGGGGTGAACACCGCCGTCTTCGAGAGGCGCCATGTGATCGGG
GGTGCAGCTGTCACTGAGGAGATCATCCCAGGGTTTAAGTTCTCCCGTGCGTCCTACCTGCTCAGCCTGC
TGAGGCCGCAGATTTACACTGATCTGGAGCTGAAGAAACATGGGCTGAGGCTTCATCTTCGAAACCCCTA
CTCCTTCACCCCCATGCTGGAAGAGGGTGCAGGCAGCAAGGTGCCCAGGTGCCTTCTGCTGGGCACAGAC
ATGGCAGAAAACCAGAAGCAGATCGCCCAGTTCTCCCAGAAGGATGCCCAGGTCTTTCCCAAATATGAGG
AGTTCATGCATCGCTTGGCATTAGCCATTGACCCTCTGCTGGATGCGGCCCCCGTGGACATGGCGGCCTT
CCAGCATGGCTCCTTGCTGCAAAGGATGAGGTCGCTCTCCACCCTCAAGCCCCTGCTGAAGGCAGGCCGC
ATCCTGGGAGCCCAGCTTCCCCGATATTATGAGGTCCTCACAGCTCCCATTACCAAGGTGCTGGATCAGT
GGTTCGAGTCTGAGCCTTTAAAAGCCACTCTAGCCACAGATGCAGTGATTGGAGCCATGACAAGTCCCCA
CACTCCGGGGAGTGGGTATGTGCTGCTGCACCATGTGATGGGGGGCCTGGAGGGAATGCAGGGGGCCTGG
GGCTACGTCCAGGGGGGCATGGGTGCCCTCTCTGATGCGATCGCAAGCTCAGCCACCACACATGGAGCAA
GCATCTTCACTGAAAAGACAGTGGCGAAGGTGCAGGTGAACAGTGAAGGCTGTGTTCAAGGAGTTGTGCT
GGAAGATGGCACAGAGGTGAGAAGCAAAATGGTGCTGTCCAACACATCACCGCAGATCACCTTCCTGAAG
CTGACGCCACAGGAGTGGCTTCCTGAGGAGTTCCTGGAGAGAATCTCTCAGCTGGACACCCGGTCGCCTG
TCACCAAGATCAATGTGGCCGTAGACAGGCTGCCCAGCTTCCTGGCGGCCCCCAATGCTCCCAGGGGCCA
GCCGCTGCCCCATCACCAATGCTCCATCCACCTGAACTGTGAAGACACCCTCCTCCTTCATCAGGCCTTT
GAAGATGCCATGGATGGCCTGTCTTCCCACAGGCCTGTGATTGAGCTCTGCATCCCTTCCTCGCTGGACC
CCACCCTGGCTCCCCCTGGCTGCCATGTAGTCTCCCTCTTCACTCAGTACACGCCCTATACGCTGGCTGG
AGGCAAGGCCTGGGACGAGCAGGAGAGAGACGCTTATGCAGACAGAGTGTTTGATTGCATCGAGGTCTAT
GCCCCTGGCTTCAAGGACTCTGTGGTTGGCAGAGACATCCTCACACCACCAGATTTGGAGAGAATCTTCG
GGCTTCCTGGAGGGAACATATTCCACTGCGCCATGTCCCTGGACCAGCTCTACTTCGCCCGCCCCGTGCC
CCTGCATTCTGGCTACCGCTGCCCTCTCCAGGGCCTGTATCTCTGTGGAAGTGGGGCTCATCCTGGAGGA
GGTGTGATGGGAGCTGCTGGGCGAAATGCAGCACATGTGGCCTTTAGGGACCTCAAGAGCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202983 protein sequence
Red=Cloning site Green=Tags(s)

MAASGRGLCKAVAASPFPAWRRDNTEARGGLKPEYDAVVIGAGHNGLVAAAYLQRLGVNTAVFERRHVIG
GAAVTEEIIPGFKFSRASYLLSLLRPQIYTDLELKKHGLRLHLRNPYSFTPMLEEGAGSKVPRCLLLGTD
MAENQKQIAQFSQKDAQVFPKYEEFMHRLALAIDPLLDAAPVDMAAFQHGSLLQRMRSLSTLKPLLKAGR
ILGAQLPRYYEVLTAPITKVLDQWFESEPLKATLATDAVIGAMTSPHTPGSGYVLLHHVMGGLEGMQGAW
GYVQGGMGALSDAIASSATTHGASIFTEKTVAKVQVNSEGCVQGVVLEDGTEVRSKMVLSNTSPQITFLK
LTPQEWLPEEFLERISQLDTRSPVTKINVAVDRLPSFLAAPNAPRGQPLPHHQCSIHLNCEDTLLLHQAF
EDAMDGLSSHRPVIELCIPSSLDPTLAPPGCHVVSLFTQYTPYTLAGGKAWDEQERDAYADRVFDCIEVY
APGFKDSVVGRDILTPPDLERIFGLPGGNIFHCAMSLDQLYFARPVPLHSGYRCPLQGLYLCGSGAHPGG
GVMGAAGRNAAHVAFRDLKSM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032709
ORF Size 1743 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032709.1, NP_116098.1
RefSeq Size 2083 bp
RefSeq ORF 1746 bp
Locus ID 84795
UniProt ID Q8N2H3
Cytogenetics 10q24.2
MW 63 kDa
Summary Probable oxidoreductase.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_032709" in other vectors (4)
SKU Description Size Price
RC202983L3 Lenti ORF clone of Human pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2), Myc-DDK-tagged 10 ug
$1,111.00
RC202983L4 Lenti ORF clone of Human pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2), mGFP tagged 10 ug
$1,111.00
RG202983 PYROXD2 (tGFP-tagged) - Human pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2) 10 ug
$1,011.00
SC320141 PYROXD2 (untagged)-Human pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2) 10 ug
$812.00

Plasmid Map for RC202983

Download sequence for

Cloning scheme for RC202983

Circular map for RC202983

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