AMPD2 (NM_139156) Human Tagged ORF Clone

SKU
RC202938
AMPD2 (Myc-DDK-tagged)-Human adenosine monophosphate deaminase 2 (AMPD2), transcript variant 2
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AMPD2
Synonyms PCH9; SPG63
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202938 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCAGAGGCTCGGGGTGGTCTGGGGGCCCCTCCGCTGCAGTCTGCCCGATCCCTGCCGGGCCCCG
CCCCCTGCCTCAAGCACTTCCCGCTCGACCTGCGCACGTCTATGGATGGCAAATGCAAGGAGATCGCCGA
GGAGCTGTTCACCCGCTCACTGGCTGAGAGCGAGCTCCGTAGTGCCCCGTATGAGTTCCCCGAGGAGAGC
CCCATTGAACAGCTGGAGGAGCGGCGGCAGCGGCTGGAGCGGCAGATCAGCCAGGATGTCAAGCTGGAGC
CAGACATCCTGCTTCGGGCCAAGCAAGATTTCCTGAAGACGGACAGTGACTCGGACCTACAGCTCTACAA
GGAACAGGGTGAGGGGCAGGGTGACCGGAGCCTGCGGGAGCGTGATGTGCTGGAACGGGAGTTTCAGCGG
GTCACCATCTCTGGGGAGGAGAAGTGTGGGGTGCCGTTCACAGACCTGCTGGATGCAGCCAAGAGTGTGG
TGCGGGCGCTCTTCATCCGGGAGAAGTACATGGCCCTGTCCCTGCAGAGCTTCTGCCCCACCACCCGCCG
CTACCTGCAGCAGCTGGCTGAAAAGCCTCTGGAGACCCGGACCTATGAACAGGGCCCCGACACCCCTGTG
TCTGCTGATGCCCCGGTGCACCCCCCTGCGCTGGAGCAGCACCCGTATGAGCACTGTGAGCCAAGCACCA
TGCCTGGGGACCTGGGCTTGGGTCTGCGCATGGTGCGGGGTGTGGTGCACGTCTACACCCGCAGGGAACC
CGACGAGCATTGCTCAGAGGTGGAGCTGCCATACCCTGACCTGCAGGAATTTGTGGCTGACGTCAATGTG
CTGATGGCCCTGATTATCAATGGCCCCATAAAGTCATTCTGCTACCGCCGGCTGCAGTACCTGAGCTCCA
AGTTCCAGATGCATGTGCTACTCAATGAGATGAAGGAGCTGGCCGCCCAGAAGAAAGTGCCACACCGAGA
TTTCTACAACATCCGCAAGGTGGACACCCACATCCATGCCTCGTCCTGCATGAACCAGAAGCATCTGCTG
CGCTTCATCAAGCGGGCAATGAAGCGGCACCTGGAGGAGATCGTGCACGTGGAGCAGGGCCGTGAACAGA
CGCTGCGGGAGGTCTTTGAGAGCATGAATCTCACGGCCTACGACCTGAGTGTGGACACGCTGGATGTGCA
TGCGGACAGGAACACTTTCCATCGCTTTGACAAGTTTAATGCCAAATACAACCCTATTGGGGAGTCCGTC
CTCCGAGAGATCTTCATCAAGACGGACAACAGGGTATCTGGGAAGTACTTTGCTCACATCATCAAGGAGG
TGATGTCAGACCTGGAGGAGAGCAAATACCAGAATGCAGAGCTGCGGCTCTCCATTTACGGGCGCTCGAG
GGATGAGTGGGACAAGCTGGCGCGCTGGGCCGTCATGCACCGCGTGCACTCCCCCAACGTGCGCTGGCTG
GTGCAGGTGCCCCGCCTCTTTGATGTGTACCGTACCAAGGGCCAGCTGGCCAACTTCCAGGAGATGCTGG
AGAACATCTTCCTGCCACTGTTCGAGGCCACTGTGCACCCTGCCAGCCACCCGGAACTGCATCTCTTCTT
AGAGCACGTGGATGGTTTTGACAGCGTGGATGATGAGTCCAAGCCTGAAAACCATGTCTTCAACCTGGAG
AGCCCCCTGCCTGAGGCGTGGGTGGAGGAGGACAACCCACCCTATGCCTACTACCTGTACTACACCTTTG
CCAACATGGCCATGTTGAACCACCTGCGCAGGCAGAGGGGCTTCCACACGTTTGTGCTGAGGCCACACTG
TGGGGAGGCTGGGCCCATCCACCACCTGGTGTCAGCCTTCATGCTGGCTGAGAACATTTCCCACGGGCTC
CTTCTGCGCAAGGCCCCCGTCCTGCAGTACCTGTACTACCTGGCCCAGATCGGCATCGCCATGTCTCCGC
TCAGCAACAACAGCCTCTTCCTCAGCTATCACCGGAATCCGCTACCGGAGTACCTGTCCCGCGGCCTCAT
GGTCTCCCTGTCCACTGATGATCCCTTGCAGTTCCACTTCACCAAGGAGCCGCTGATGGAGGAGTACAGC
ATCGCCACCCAGGTGTGGAAGCTCAGCTCCTGCGATATGTGTGAGCTGGCCCGCAACAGCGTGCTCATGA
GCGGCTTCTCGCACAAGGTAAAGAGCCACTGGCTGGGACCCAACTATACCAAGGAAGGCCCTGAGGGGAA
TGACATCCGCCGGACCAATGTGCCAGACATCCGCGTGGGCTACCGCTACGAGACCCTGTGCCAGGAGCTG
GCGCTCATCACGCAGGCAGTCCAGAGTGAGATGCTGGAGACCATTCCAGAGGAGGCGGGTATCACCATGA
GCCCAGGGCCTCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202938 protein sequence
Red=Cloning site Green=Tags(s)

MASEARGGLGAPPLQSARSLPGPAPCLKHFPLDLRTSMDGKCKEIAEELFTRSLAESELRSAPYEFPEES
PIEQLEERRQRLERQISQDVKLEPDILLRAKQDFLKTDSDSDLQLYKEQGEGQGDRSLRERDVLEREFQR
VTISGEEKCGVPFTDLLDAAKSVVRALFIREKYMALSLQSFCPTTRRYLQQLAEKPLETRTYEQGPDTPV
SADAPVHPPALEQHPYEHCEPSTMPGDLGLGLRMVRGVVHVYTRREPDEHCSEVELPYPDLQEFVADVNV
LMALIINGPIKSFCYRRLQYLSSKFQMHVLLNEMKELAAQKKVPHRDFYNIRKVDTHIHASSCMNQKHLL
RFIKRAMKRHLEEIVHVEQGREQTLREVFESMNLTAYDLSVDTLDVHADRNTFHRFDKFNAKYNPIGESV
LREIFIKTDNRVSGKYFAHIIKEVMSDLEESKYQNAELRLSIYGRSRDEWDKLARWAVMHRVHSPNVRWL
VQVPRLFDVYRTKGQLANFQEMLENIFLPLFEATVHPASHPELHLFLEHVDGFDSVDDESKPENHVFNLE
SPLPEAWVEEDNPPYAYYLYYTFANMAMLNHLRRQRGFHTFVLRPHCGEAGPIHHLVSAFMLAENISHGL
LLRKAPVLQYLYYLAQIGIAMSPLSNNSLFLSYHRNPLPEYLSRGLMVSLSTDDPLQFHFTKEPLMEEYS
IATQVWKLSSCDMCELARNSVLMSGFSHKVKSHWLGPNYTKEGPEGNDIRRTNVPDIRVGYRYETLCQEL
ALITQAVQSEMLETIPEEAGITMSPGPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_139156
ORF Size 2394 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139156.3
RefSeq Size 3785 bp
RefSeq ORF 2397 bp
Locus ID 271
UniProt ID Q01433
Cytogenetics 1p13.3
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Purine metabolism
MW 92.1 kDa
Summary The protein encoded by this gene is important in purine metabolism by converting AMP to IMP. The encoded protein, which acts as a homotetramer, is one of three AMP deaminases found in mammals. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
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Citations

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