PLTP (NM_182676) Human Tagged ORF Clone

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SKU
RC202925
PLTP (Myc-DDK-tagged)-Human phospholipid transfer protein (PLTP), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLTP
Synonyms BPIFE; HDLCQ9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202925 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCAGGCTGCAAGA
TCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGCTTTCTGGAGCAAGAGCTGGA
GACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCACTTCTACTACAACATCTCTGAGGTGAAGGTC
ACAGAGCTGCAACTGACATCTTCCGAGCTCGATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCA
ATGCCTCCTTGGGGCTGCGCTTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTC
CACGTTCATCACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTTGTTGGCATTG
ACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATGGACTTCCGGGGGGCCTTCTT
CCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCAGTGGAGCCCCAGCTGCAGGAGGAAGAGCGG
ATGGTGTATGTGGCCTTCTCTGAGTTCTTCTTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCC
TGCAGCTGTTGCTGGTGGGGGACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGG
GAGCATTGTCCTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATTGCCCTGGTCC
CACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGTCTCAGCGCCAAGATGGCTCT
CCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGGTTCCGAATCTATTCCAACCATTCTGCACTG
GAGTCGCTGGCTCTGATCCCATTACAGGCCCCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGC
TCAATGAGCGGACCTGGCGTGGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGT
GACGAACCATGCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCTGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202925 protein sequence
Red=Cloning site Green=Tags(s)

MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGHFYYNISEVKV
TELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFLKVYDFLSTFITSGMRFLLNQQICPVLYHAG
TVLLNSLLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEER
MVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAP
PRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSAL
ESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVI
EKNRPADVRASTAPTPSTAAV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182676
ORF Size 1323 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182676.2
RefSeq Size 1944 bp
RefSeq ORF 1326 bp
Locus ID 5360
UniProt ID P55058, P55058-2
Cytogenetics 20q13.12
Protein Families Druggable Genome, Secreted Protein
Protein Pathways PPAR signaling pathway
MW 49.3 kDa
Summary The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_182676" in other vectors (4)
SKU Description Size Price
RC202925L3 Lenti ORF clone of Human phospholipid transfer protein (PLTP), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC202925L4 Lenti ORF clone of Human phospholipid transfer protein (PLTP), transcript variant 2, mGFP tagged 10 ug
$757.00
RG202925 PLTP (tGFP-tagged) - Human phospholipid transfer protein (PLTP), transcript variant 2 10 ug
$489.00 $657.00
SC321522 PLTP (untagged)-Human phospholipid transfer protein (PLTP), transcript variant 2 10 ug
$457.00

Plasmid Map for RC202925

Download sequence for

Cloning scheme for RC202925

Circular map for RC202925

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