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CELA2A (NM_033440) Human Tagged ORF Clone

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SKU
RC202746
CELA2A (Myc-DDK-tagged)-Human chymotrypsin-like elastase family, member 2A (CELA2A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CELA2A
Synonyms AOMS4; ELA2A; PE-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202746 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAAGGACGCTGCTGCTGTCCACTTTGGTGGCTGGAGCCCTCAGTTGTGGGGACCCCACTTACCCAC
CTTATGTGACTAGGGTGGTTGGCGGTGAAGAAGCGAGGCCCAACAGCTGGCCCTGGCAGGTCTCCCTGCA
GTACAGCTCCAATGGCAAGTGGTACCACACCTGCGGAGGGTCCCTGATAGCCAACAGCTGGGTCCTGACG
GCTGCCCACTGCATCAGCTCCTCCAGGACCTACCGCGTGGGGCTGGGCCGGCACAACCTCTACGTTGCGG
AGTCCGGCTCGCTGGCAGTCAGTGTCTCTAAGATTGTGGTGCACAAGGACTGGAACTCCAACCAAATCTC
CAAAGGGAACGACATTGCCCTGCTCAAACTGGCTAACCCCGTCTCCCTCACCGACAAGATCCAGCTGGCC
TGCCTCCCTCCTGCCGGCACCATTCTACCCAACAACTACCCCTGCTACGTCACGGGCTGGGGAAGGCTGC
AGACCAACGGGGCTGTTCCTGATGTCCTGCAGCAGGGCCGGTTGCTGGTTGTGGACTATGCCACCTGCTC
CAGCTCTGCCTGGTGGGGCAGCAGCGTGAAAACCAGTATGATCTGTGCTGGGGGTGATGGCGTGATCTCC
AGCTGCAACGGAGACTCTGGCGGGCCACTGAACTGTCAGGCGTCTGACGGCCGGTGGCAGGTGCACGGCA
TCGTCAGCTTCGGGTCTCGCCTCGGCTGCAACTACTACCACAAGCCCTCCGTCTTCACGCGGGTCTCCAA
TTACATCGACTGGATCAATTCGGTGATTGCAAATAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202746 protein sequence
Red=Cloning site Green=Tags(s)

MIRTLLLSTLVAGALSCGDPTYPPYVTRVVGGEEARPNSWPWQVSLQYSSNGKWYHTCGGSLIANSWVLT
AAHCISSSRTYRVGLGRHNLYVAESGSLAVSVSKIVVHKDWNSNQISKGNDIALLKLANPVSLTDKIQLA
CLPPAGTILPNNYPCYVTGWGRLQTNGAVPDVLQQGRLLVVDYATCSSSAWWGSSVKTSMICAGGDGVIS
SCNGDSGGPLNCQASDGRWQVHGIVSFGSRLGCNYYHKPSVFTRVSNYIDWINSVIANN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033440
ORF Size 807 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033440.3
RefSeq Size 934 bp
RefSeq ORF 810 bp
Locus ID 63036
UniProt ID P08217
Cytogenetics 1p36.21
Protein Families Druggable Genome, Protease, Secreted Protein
MW 28.9 kDa
Summary Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. provided by RefSeq, May 2009
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"NM_033440" in other vectors (6)
SKU Description Size Price
RC202746L1 Lenti ORF clone of Human chymotrypsin-like elastase family, member 2A (CELA2A), Myc-DDK-tagged 10 ug
$600.00
RC202746L2 Lenti ORF clone of Human chymotrypsin-like elastase family, member 2A (CELA2A), mGFP tagged 10 ug
$600.00
RC202746L3 Lenti ORF clone of Human chymotrypsin-like elastase family, member 2A (CELA2A), Myc-DDK-tagged 10 ug
$600.00
RC202746L4 Lenti ORF clone of Human chymotrypsin-like elastase family, member 2A (CELA2A), mGFP tagged 10 ug
$600.00
RG202746 CELA2A (tGFP-tagged) - Human chymotrypsin-like elastase family, member 2A (CELA2A) 10 ug
$489.00 $500.00
SC123089 CELA2A (untagged)-Human chymotrypsin-like elastase family, member 2A (CELA2A) 10 ug
$300.00

Plasmid Map for RC202746

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202746
Circular map for RC202746

file-alt Citations

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