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COMMD10 (NM_016144) Human Tagged ORF Clone

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SKU
RC202655
COMMD10 (Myc-DDK-tagged)-Human COMM domain containing 10 (COMMD10)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COMMD10
Synonyms PTD002
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202655 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTCCCCGCGGCGCTGATCCTACGGGAGAGCCCCAGCATGAAGAAAGCAGTGTCACTGATAAATG
CAATAGATACAGGAAGATTTCCACGGTTGCTCACTCGGATTCTTCAAAAACTTCACCTGAAGGCTGAGAG
CAGTTTCAGTGAAGAAGAGGAAGAAAAACTTCAAGCGGCATTTTCTCTAGAGAAACAAGATCTTCACCTA
GTTCTTGAAACAATATCATTTATTTTAGAACAGGCAGTGTATCACAATGTGAAGCCAGCAGCTTTGCAGC
AGCAATTAGAGAACATTCATCTTAGACAAGACAAAGCTGAAGCATTTGTCAATACGTGGTCTTCTATGGG
TCAAGAAACAGTTGAAAAGTTCCGGCAGAGAATTCTGGCTCCCTGTAAGCTAGAGACCGTTGGATGGCAG
CTTAACCTTCAGATGGCTCACTCTGCTCAAGCAAAACTAAAATCTCCTCAAGCTGTGTTACAACTCGGAG
TGAACAATGAAGATTCAAAGAGCCTGGAGAAAGTTCTTGTGGAATTCAGTCACAAGGAGTTGTTTGATTT
CTATAACAAGCTAGAGACTATACAAGCACAGCTGGATTCCCTTACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202655 protein sequence
Red=Cloning site Green=Tags(s)

MAVPAALILRESPSMKKAVSLINAIDTGRFPRLLTRILQKLHLKAESSFSEEEEEKLQAAFSLEKQDLHL
VLETISFILEQAVYHNVKPAALQQQLENIHLRQDKAEAFVNTWSSMGQETVEKFRQRILAPCKLETVGWQ
LNLQMAHSAQAKLKSPQAVLQLGVNNEDSKSLEKVLVEFSHKELFDFYNKLETIQAQLDSLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016144
ORF Size 606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016144.4
RefSeq Size 1456 bp
RefSeq ORF 609 bp
Locus ID 51397
UniProt ID Q9Y6G5
Cytogenetics 5q23.1
MW 23 kDa
Summary May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes (PubMed:21778237). May down-regulate activation of NF-kappa-B (PubMed:15799966).UniProtKB/Swiss-Prot Function
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"NM_016144" in other vectors (4)
SKU Description Size Price
RC202655L3 Lenti ORF clone of Human COMM domain containing 10 (COMMD10), Myc-DDK-tagged 10 ug
$600.00
RC202655L4 Lenti ORF clone of Human COMM domain containing 10 (COMMD10), mGFP tagged 10 ug
$600.00
RG202655 COMMD10 (tGFP-tagged) - Human COMM domain containing 10 (COMMD10) 10 ug
$489.00 $500.00
SC114480 COMMD10 (untagged)-Human COMM domain containing 10 (COMMD10) 10 ug
$300.00

Plasmid Map for RC202655

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202655
Circular map for RC202655

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