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ZNF71 (NM_021216) Human Tagged ORF Clone

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SKU
RC202614
ZNF71 (Myc-DDK-tagged)-Human zinc finger protein 71 (ZNF71)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF71
Synonyms EZFIT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202614 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGAGTTGGATCCAAAGAATGACATTTCGGAAGACAAGCTCTCCGTTGTTGGGGAGGCCACGGGGG
GACCCACGAGGAATGGTGCCAGGGGTCCTGGCTCAGAAGGAGTGTGGGAACCAGGCAGCTGGCCAGAGAG
GCCGCGGGGAGATGCAGGTGCAGAGTGGGAGCCATTGGGAATTCCCCAGGGGAACAAACTCTTAGGGGGC
TCAGTACCCGCATGTCATGAACTGAAGGCATTTGCCAACCAAGGCTGTGTCCTGGTCCCACCACGGCTGG
ACGACCCCACAGAAAAGGGGGCCTGTCCACCCGTAAGGCGTGGCAAGAACTTCTCCAGCACTTCAGACCT
CAGTAAGCCCCCCATGCCCTGCGAGGAGAAGAAAACCTACGACTGCAGCGAGTGTGGCAAGGCCTTTAGC
CGAAGCTCGTCCCTGATAAAGCACCAAAGGATCCACACGGGAGAAAAGCCGTTTGAGTGTGACACCTGTG
GGAAGCACTTCATCGAGCGCTCGTCCCTCACCATCCACCAGCGGGTGCACACGGGCGAGAAGCCCTATGC
CTGCGGGGACTGCGGCAAGGCCTTCAGCCAGCGCATGAACCTCACTGTGCACCAGCGCACGCACACGGGC
GAGAAGCCGTATGTGTGCGACGTGTGTGGCAAGGCCTTCCGGAAGACTTCCTCTCTCACCCAGCACGAGC
GGATCCACACGGGGGAGAAGCCCTACGCGTGCGGGGACTGCGGCAAGGCCTTCAGCCAGAACATGCACCT
CATCGTGCACCAGCGCACGCACACCGGGGAGAAGCCGTACGTGTGCCCCGAGTGCGGGCGAGCCTTCAGC
CAGAACATGCACCTGACCGAGCACCAGCGCACGCACACCGGGGAGAAGCCGTACGCCTGCAAGGAGTGCG
GCAAGGCCTTCAACAAGAGCTCCTCTCTCACCCTGCACCAGAGGAACCACACCGGCGAGAAGCCCTACGT
GTGCGGCGAGTGCGGCAAGGCCTTCAGCCAGAGCTCCTACCTCATCCAGCACCAGCGCTTCCACATCGGC
GTGAAGCCGTTCGAGTGCAGCGAGTGCGGCAAGGCCTTCAGCAAGAACTCCTCGCTCACGCAGCACCAGC
GCATCCACACCGGCGAGAAGCCCTACGAGTGCTACATCTGCAAGAAGCACTTCACGGGGCGCTCGTCCCT
CATCGTGCACCAGATCGTGCACACCGGGGAGAAGCCCTACGTGTGCGGCGAGTGCGGCAAGGCCTTCAGC
CAGAGCGCCTACCTCATCGAGCACCAGCGGATCCACACCGGCGAGAAGCCGTACAGGTGCGGCCAGTGCG
GGAAGTCCTTCATCAAGAACTCCTCCCTCACTGTGCACCAGCGGATCCACACGGGCGAGAAGCCCTACCG
ATGCGGCGAGTGCGGGAAGACCTTCAGCCGCAACACGAACCTGACGCGCCACCTGCGGATTCACACC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202614 protein sequence
Red=Cloning site Green=Tags(s)

MKELDPKNDISEDKLSVVGEATGGPTRNGARGPGSEGVWEPGSWPERPRGDAGAEWEPLGIPQGNKLLGG
SVPACHELKAFANQGCVLVPPRLDDPTEKGACPPVRRGKNFSSTSDLSKPPMPCEEKKTYDCSECGKAFS
RSSSLIKHQRIHTGEKPFECDTCGKHFIERSSLTIHQRVHTGEKPYACGDCGKAFSQRMNLTVHQRTHTG
EKPYVCDVCGKAFRKTSSLTQHERIHTGEKPYACGDCGKAFSQNMHLIVHQRTHTGEKPYVCPECGRAFS
QNMHLTEHQRTHTGEKPYACKECGKAFNKSSSLTLHQRNHTGEKPYVCGECGKAFSQSSYLIQHQRFHIG
VKPFECSECGKAFSKNSSLTQHQRIHTGEKPYECYICKKHFTGRSSLIVHQIVHTGEKPYVCGECGKAFS
QSAYLIEHQRIHTGEKPYRCGQCGKSFIKNSSLTVHQRIHTGEKPYRCGECGKTFSRNTNLTRHLRIHT

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_021216
ORF Size 1467 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021216.3, NP_067039.1
RefSeq Size 3144 bp
RefSeq ORF 1470 bp
Locus ID 58491
UniProt ID Q9NQZ8
Cytogenetics 19q13.43
Domains zf-C2H2
Protein Families Druggable Genome, Transcription Factors
MW 54.5 kDa
Summary May be involved in transcriptional regulation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021216" in other vectors (4)
SKU Description Size Price
RC202614L3 Lenti ORF clone of Human zinc finger protein 71 (ZNF71), Myc-DDK-tagged 10 ug
$757.00
RC202614L4 Lenti ORF clone of Human zinc finger protein 71 (ZNF71), mGFP tagged 10 ug
$757.00
RG202614 ZNF71 (tGFP-tagged) - Human zinc finger protein 71 (ZNF71) 10 ug
$657.00
SC112935 ZNF71 (untagged)-Human zinc finger protein 71 (ZNF71) 10 ug
$457.00

Plasmid Map for RC202614

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202614
Circular map for RC202614

file-alt Citations

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