OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

UBE2D4 (NM_015983) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC202473
UBE2D4 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA810786 UBE2D4 mouse monoclonal antibody,clone OTI1B1 100 ul
$447.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "UBE2D4 or NM_015983"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UBE2D4
Synonyms HBUCE1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202473 representing NM_015983
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTAAAGCGGATCCAGAAGGAATTAACCGACTTGCAGAGGGATCCTCCTGCCCAGTGTTCTGCAG
GACCTGTCGGTGATGACTTGTTCCACTGGCAGGCCACCATCATGGGCCCGAATGACAGTCCTTACCAAGG
AGGTGTTTTCTTCCTGACCATCCACTTTCCTACAGATTACCCGTTCAAGCCCCCAAAGGTTGCTTTCACA
ACCAAAATTTATCACCCTAATATCAACAGCAATGGCAGCATCTGCCTTGATATCCTGCGGTCTCAGTGGT
CTCCAGCGTTGACTGTGTCAAAAGTTCTCTTGTCCATCTGCTCGCTGCTCTGCGACCCCAACCCCGATGA
CCCCCTGGTGCCAGAGATAGCACACACCTACAAGGCCGACAGAGAGAAGTACAACAGACTAGCAAGAGAG
TGGACACAAAAATATGCTATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202473 representing NM_015983
Red=Cloning site Green=Tags(s)

MAVKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFT
TKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLARE
WTQKYAM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015983
ORF Size 441 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015983.4
RefSeq Size 1398 bp
RefSeq ORF 444 bp
Locus ID 51619
UniProt ID Q9Y2X8
Cytogenetics 7p13
Domains UBCc
Protein Pathways Ubiquitin mediated proteolysis
MW 16.5 kDa
Summary Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_015983" in other vectors (6)
SKU Description Size Price
RC202473L1 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), Myc-DDK-tagged 10 ug
$450.00
RC202473L2 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), mGFP tagged 10 ug
$450.00
RC202473L3 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), Myc-DDK-tagged 10 ug
$450.00
RC202473L4 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), mGFP tagged 10 ug
$450.00
RG202473 UBE2D4 (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4) 10 ug
$489.00
SC114559 UBE2D4 (untagged)-Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4) 10 ug
$150.00

Plasmid Map for RC202473

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202473
Circular map for RC202473

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products