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GRK6 (NM_001004106) Human Tagged ORF Clone

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SKU
RC202464
GRK6 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $804.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GRK6
Synonyms GPRK6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202464 representing NM_001004106
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTCGAGAACATCGTAGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGCGGTGGAAATCGCA
AAGGCAAAAGCAAGAAATGGCGGCAGATGCTCCAGTTCCCTCACATCAGCCAGTGCGAAGAGCTGCGGCT
CAGCCTCGAGCGTGACTATCACAGCCTGTGCGAGCGGCAGCCCATTGGGCGCCTGCTGTTCCGAGAGTTC
TGTGCCACGAGGCCGGAGCTGAGCCGCTGCGTCGCCTTCCTGGATGGGGTGGCCGAGTATGAAGTGACCC
CGGATGACAAGCGGAAGGCATGTGGGCGGCAGCTAACGCAGAATTTTCTGAGCCACACGGGTCCTGACCT
CATCCCTGAGGTCCCCCGGCAGCTGGTGACGAACTGCACCCAGCGGCTGGAGCAGGGTCCCTGCAAAGAC
CTTTTCCAGGAACTCACCCGGCTGACCCACGAGTACCTGAGCGTGGCCCCTTTTGCCGACTACCTCGACA
GCATCTACTTCAACCGTTTCCTGCAGTGGAAGTGGCTGGAAAGGCAGCCAGTGACCAAAAACACCTTCAG
GCAATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTGTGCGCCTGCCAGGTGCGGGCCACAGGTAAG
ATGTATGCCTGCAAGAAGCTAGAGAAAAAGCGGATCAAGAAGCGGAAAGGGGAGGCCATGGCGCTGAACG
AGAAGCAGATCCTGGAGAAAGTGAACAGTAGGTTTGTAGTGAGCTTGGCCTACGCCTATGAGACCAAGGA
CGCGCTGTGCCTGGTGCTGACACTGATGAACGGGGGCGACCTCAAGTTCCACATCTACCACATGGGCCAG
GCTGGCTTCCCCGAAGCGCGGGCCGTCTTCTACGCCGCCGAGATCTGCTGTGGCCTGGAGGACCTGCACC
GGGAGCGCATCGTGTACAGGGACCTGAAGCCCGAGAACATCTTGCTGGATGACCACGGCCACATCCGCAT
CTCTGACCTGGGACTAGCTGTGCATGTGCCCGAGGGCCAGACCATCAAAGGGCGTGTGGGCACCGTGGGT
TACATGGCTCCGGAGGTGGTGAAGAATGAACGGTACACGTTCAGCCCTGACTGGTGGGCGCTCGGCTGCC
TCCTGTACGAGATGATCGCAGGCCAGTCGCCCTTCCAGCAGAGGAAGAAGAAGATCAAGCGGGAGGAGGT
GGAGCGGCTGGTGAAGGAGGTCCCCGAGGAGTATTCCGAGCGCTTTTCCCCGCAGGCCCGCTCACTTTGC
TCACAGCTCCTCTGCAAGGACCCTGCCGAACGCCTGGGGTGTCGTGGGGGCAGTGCCCGCGAGGTGAAGG
AGCACCCCCTCTTTAAGAAGCTGAACTTCAAGCGGCTGGGAGCTGGCATGCTGGAGCCGCCGTTCAAGCC
TGACCCCCAGGCCATTTACTGCAAGGATGTTCTGGACATTGAACAGTTCTCTACGGTCAAGGGCGTGGAG
CTGGAGCCTACCGACCAGGACTTCTACCAGAAGTTTGCCACAGGCAGTGTGCCCATCCCCTGGCAGAACG
AGATGGTGGAGACCGAGTGCTTCCAAGAGCTGAATGTCTTTGGGCTGGATGGCTCAGTTCCCCCAGACCT
GGACTGGAAGGGCCAGCCACCTGCACCTCCTAAAAAGGGACTGCTGCAGAGACTCTTCAGTCGCCAAGAT
TGCTGTGGAAACTGCAGCGACAGCGAGGAAGAGCTCCCCACCCGCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202464 representing NM_001004106
Red=Cloning site Green=Tags(s)

MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREF
CATRPELSRCVAFLDGVAEYEVTPDDKRKACGRQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKD
LFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGK
MYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQ
AGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVG
YMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSERFSPQARSLC
SQLLCKDPAERLGCRGGSAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVE
LEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQD
CCGNCSDSEEELPTRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004106
ORF Size 1728 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004106.3
RefSeq Size 2930 bp
RefSeq ORF 1731 bp
Locus ID 2870
UniProt ID P43250
Cytogenetics 5q35.3
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Endocytosis
MW 65.8 kDa
Summary This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001004106" in other vectors (7)
SKU Description Size Price
RC202464L1 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, Myc-DDK-tagged 10 ug
$1,104.00
RC202464L2 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, mGFP tagged 10 ug
$1,104.00
RC202464L3 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, Myc-DDK-tagged 10 ug
$1,104.00
RC202464L4 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, mGFP tagged 10 ug
$1,104.00
RG202464 GRK6 (tGFP-tagged) - Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1 10 ug
$489.00 $1,004.00
SC300602 GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1 10 ug
$859.00
SC320538 GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1 10 ug
$805.00

Plasmid Map for RC202464

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202464
Circular map for RC202464

file-alt Citations

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