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GRK6 (NM_001004106) Human Untagged Clone

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SKU
SC320538
GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1
  $805.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GRK6
Synonyms GPRK6
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_001004106.1 CCGAGCCGCGCCGAGCCGCGCCGATCGCCATCCGGCCTCGGCACTCGCGCGCGATCCCGG
CCGGCGGCGCGGCCCGGCGGGCCAGGCGGCGCCACAGCCCATGGAGCTCGAGAACATCGT
AGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGCGGTGGAAATCGCAAAGGCAAAAG
CAAGAAATGGCGGCAGATGCTCCAGTTCCCTCACATCAGCCAGTGCGAAGAGCTGCGGCT
CAGCCTCGAGCGTGACTATCACAGCCTGTGCGAGCGGCAGCCCATTGGGCGCCTGCTGTT
CCGAGAGTTCTGTGCCACGAGGCCGGAGCTGAGCCGCTGCGTCGCCTTCCTGGATGGGGT
GGCCGAGTATGAAGTGACCCCGGATGACAAGCGGAAGGCATGTGGGCGGCAGCTAACGCA
GAATTTTCTGAGCCACACGGGTCCTGACCTCATCCCTGAGGTCCCCCGGCAGCTGGTGAC
GAACTGCACCCAGCGGCTGGAGCAGGGTCCCTGCAAAGACCTTTTCCAGGAACTCACCCG
GCTGACCCACGAGTACCTGAGCGTGGCCCCTTTTGCCGACTACCTCGACAGCATCTACTT
CAACCGTTTCCTGCAGTGGAAGTGGCTGGAAAGGCAGCCAGTGACCAAAAACACCTTCAG
GCAATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTGTGCGCCTGCCAGGTGCGGGC
CACAGGTAAGATGTATGCCTGCAAGAAGCTAGAGAAAAAGCGGATCAAGAAGCGGAAAGG
GGAGGCCATGGCGCTGAACGAGAAGCAGATCCTGGAGAAAGTGAACAGTAGGTTTGTAGT
GAGCTTGGCCTACGCCTATGAGACCAAGGACGCGCTGTGCCTGGTGCTGACACTGATGAA
CGGGGGCGACCTCAAGTTCCACATCTACCACATGGGCCAGGCTGGCTTCCCCGAAGCGCG
GGCCGTCTTCTACGCCGCCGAGATCTGCTGTGGCCTGGAGGACCTGCACCGGGAGCGCAT
CGTGTACAGGGACCTGAAGCCCGAGAACATCTTGCTGGATGACCACGGCCACATCCGCAT
CTCTGACCTGGGACTAGCTGTGCATGTGCCCGAGGGCCAGACCATCAAAGGGCGTGTGGG
CACCGTGGGTTACATGGCTCCGGAGGTGGTGAAGAATGAACGGTACACGTTCAGCCCTGA
CTGGTGGGCGCTCGGCTGCCTCCTGTACGAGATGATCGCAGGCCAGTCGCCCTTCCAGCA
GAGGAAGAAGAAGATCAAGCGGGAGGAGGTGGAGCGGCTGGTGAAGGAGGTCCCCGAGGA
GTATTCCGAGCGCTTTTCCCCGCAGGCCCGCTCACTTTGCTCACAGCTCCTCTGCAAGGA
CCCTGCCGAACGCCTGGGGTGTCGTGGGGGCAGTGCCCGCGAGGTGAAGGAGCACCCCCT
CTTTAAGAAGCTGAACTTCAAGCGGCTGGGAGCTGGCATGCTGGAGCCGCCGTTCAAGCC
TGACCCCCAGGCCATTTACTGCAAGGATGTTCTGGACATTGAACAGTTCTCTACGGTCAA
GGGCGTGGAGCTGGAGCCTACCGACCAGGACTTCTACCAGAAGTTTGCCACAGGCAGTGT
GCCCATCCCCTGGCAGAACGAGATGGTGGAGACCGAGTGCTTCCAAGAGCTGAATGTCTT
TGGGCTGGATGGCTCAGTTCCCCCAGACCTGGACTGGAAGGGCCAGCCACCTGCACCTCC
TAAAAAGGGACTGCTGCAGAGACTCTTCAGTCGCCAAGATTGCTGTGGAAACTGCAGCGA
CAGCGAGGAAGAGCTCCCCACCCGCCTCTAGCCCCCAGCCCGAGGCCCCCACCAGCAGTT
GGCGGTAGCAGCTACTCCGAGCGCCGTTTACAGTTTTGCACAGTGATCTTCCCCATTGTC
CACTCAAGTCGTGGCCTGGGGAACACAGACGGAGCTGTCCCCAGTGTCCTCCGTCCCTCA
GCCCCTGGCCTGGCTGAGTTTGGCAGGGCCTGGGCCATCCCTGGGACAAAGGTGCGTCCC
TTCAGCTCTTCTCCGTGGAGCTCGGGGCTTTCTGTATTTATGTATTTGTACGAATGTATA
TAGCGACCAGAGCATTCTTAATTCCCGCCGCAGACCTGGCGCCCCCGCCTTGGCTCCTGG
GGGCAGCCAGCCCTGGCTGGGAGAGCGGGAGCTGGCAGAGGAGCCACTGCCAAACTCAAG
GCTCCTCTGGCCCAGCTTGGATGGCTGAGGGTGGTCACACCCCTGAGCCTTCAGCACTGT
GCTGGCCACCCCGGCCTCTGAGTAAGACTCGTGCCTCCCCCTGCTGCCCTGGGCTCAGGC
TGCTACCCTCTGGGGCCCAAAGCTGTCCCTTCTCAGTGCTTGTCAGCGCTGGGTCTGGGG
CCTCTGTATGCCCTAGGCCTGTGCCAAAGTGGCCAGAGATTGGGCTGCCTGTGATACCCA
TCAGCCCACTGCCCCGGCCGGCCCAGATAGGTCTGCCTCTGCCTTCCAGCTCCCACAGCC
TGGTCCCTGATACTGGGCTCTGTCCTGCAGACACCTCTTTCAGAAACGCCCAAGCCCAGC
CCCTAGGAGGGGGTGGGGCATCCCTGGTCAACCCTCAAACATTCCGGACTCCCCTCATAA
CAATAGACACATGTGCCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
Restriction Sites Please inquire |
ACCN NM_001004106
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004106.1, NP_001004106.1
RefSeq Size 2930 bp
RefSeq ORF 1731 bp
Locus ID 2870
UniProt ID P43250
Cytogenetics 5q35.3
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Endocytosis
Summary This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) uses an alternate acceptor splice site in the last exon, compared to variant 2, resulting in a shorter isoform (A) compared to isoform B.
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Documents
Resources
"NM_001004106" in other vectors (7)
SKU Description Size Price
RC202464 GRK6 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1 10 ug
$804.00
RC202464L1 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, Myc-DDK-tagged 10 ug
$1,104.00
RC202464L2 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, mGFP tagged 10 ug
$1,104.00
RC202464L3 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, Myc-DDK-tagged 10 ug
$1,104.00
RC202464L4 Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, mGFP tagged 10 ug
$1,104.00
RG202464 GRK6 (tGFP-tagged) - Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1 10 ug
$489.00 $1,004.00
SC300602 GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1 10 ug
$859.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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