MECP2 (NM_004992) Human Tagged ORF Clone
SKU
RC202382
MECP2 (Myc-DDK-tagged)-Human methyl CpG binding protein 2 (Rett syndrome) (MECP2), transcript variant 1
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MECP2 |
| Synonyms | AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004992 |
| ORF Size | 1458 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004992.4 |
| RefSeq Size | 10182 bp |
| RefSeq ORF | 1461 bp |
| Locus ID | 4204 |
| UniProt ID | P51608, D3YJ43, Q59FJ6 |
| Cytogenetics | Xq28 |
| Protein Families | Druggable Genome |
| MW | 52.3 kDa |
| Summary | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Oct 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004992" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202382L1 | Lenti ORF clone of Human methyl CpG binding protein 2 (Rett syndrome) (MECP2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202382L2 | Lenti ORF clone of Human methyl CpG binding protein 2 (Rett syndrome) (MECP2), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC202382L3 | Lenti ORF clone of Human methyl CpG binding protein 2 (Rett syndrome) (MECP2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202382L4 | Lenti ORF clone of Human methyl CpG binding protein 2 (Rett syndrome) (MECP2), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG202382 | MECP2 (tGFP-tagged) - Human methyl CpG binding protein 2 (Rett syndrome) (MECP2), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
| SC303562 | MECP2 (untagged)-Human methyl CpG binding protein 2 (Rett syndrome) (MECP2), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
MeCP2 duplication causes hyperandrogenism by upregulating LHCGR and downregulating RORα
Wang, Wu, Zheng et al
Cell Death Dis (2021) 12 (11), 999 DOI: 10.1038/s41419-021-04277-4
Wang, Wu, Zheng et al
Cell Death Dis (2021) 12 (11), 999 DOI: 10.1038/s41419-021-04277-4
Cited by 8
AI Summary: OriGene's Human MECP2-e1 cDNA (RC202382) was used in this study to construct plasmids for investigating the molecular mechanisms by which MECP2 duplication alters androgen synthesis and regulation in a mouse model.
Snippet: quivalent amounts of protein were detected by Western blot with rabbit anti-MeCP2 antibody (Cell Signaling Technology;Cat# 3456;1:1000). Plasmid construction Human MECP2-e1 cDNA was purchased from Origene (RC202382). The Rorα expression plasmid containing mouse Rorα cDNA was amplified by PCR and subcloned into the EcoRI/KpnI sites of pCMS-EGFP plasmid. Seven different regions of mouse Lhcgr-p
MicroRNA-197 controls ADAM10 expression to mediate MeCP2's role in the differentiation of neuronal progenitors
Wang, Zheng, Yang et al
Cell Death Differ (2019) 26 (10), 1863-1879 DOI: 10.1038/s41418-018-0257-6
Wang, Zheng, Yang et al
Cell Death Differ (2019) 26 (10), 1863-1879 DOI: 10.1038/s41418-018-0257-6
Cited by 28
AI Summary: In this study, OriGene's MeCP2 (Cat# RC217678) and ADAM10 (Cat# RC200195) expression plasmids were used to investigate how MeCP2 and ADAM10 regulate neural progenitor cell differentiation via the miR-197 pathway.
Showing 1-2 of 2 papers.
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