EZH1 (NM_001991) Human Tagged ORF Clone
$1,026.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | EZH1 |
| Synonyms | KMT6B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001991 |
| ORF Size | 2241 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001991.5 |
| RefSeq Size | 4640 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 2145 |
| UniProt ID | Q92800 |
| Cytogenetics | 17q21.2 |
| Domains | myb_DNA-binding, SET |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 85.1 kDa |
| Summary | EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 PubMed 19026780).supplied by OMIM, Mar 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001991" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202367L1 | Lenti ORF clone of Human enhancer of zeste homolog 1 (Drosophila) (EZH1), Myc-DDK-tagged | 10 ug |
$1,326.00
|
|
| RC202367L2 | Lenti ORF clone of Human enhancer of zeste homolog 1 (Drosophila) (EZH1), mGFP tagged | 10 ug |
$1,326.00
|
|
| RC202367L3 | Lenti ORF clone of Human enhancer of zeste homolog 1 (Drosophila) (EZH1), Myc-DDK-tagged | 10 ug |
$1,326.00
|
|
| RC202367L4 | Lenti ORF clone of Human enhancer of zeste homolog 1 (Drosophila) (EZH1), mGFP tagged | 10 ug |
$1,326.00
|
|
| RG202367 | EZH1 (tGFP-tagged) - Human enhancer of zeste homolog 1 (Drosophila) (EZH1) | 10 ug |
$1,226.00
|
|
| SC118923 | EZH1 (untagged)-Human enhancer of zeste homolog 1 (Drosophila) (EZH1) | 10 ug |
$1,096.00
|
|
Citations
Angioplasty induces epigenomic remodeling in injured arteries
Zhang, Urabe, Ozer et al
Life Sci Alliance (2022) 5 (5) DOI: 10.26508/lsa.202101114
Zhang, Urabe, Ozer et al
Life Sci Alliance (2022) 5 (5) DOI: 10.26508/lsa.202101114
Cited by 9
AI Summary: OriGene’s EZH1 (Cat. no. RC202367) and EZH2 (Cat. no. RC202054) cDNA clones were used to enable overexpression studies, facilitating investigation of epigenetic regulators in injured arteries.
Snippet: …For EZH overexpression, EZH1 and EZH2 cDNA clones were purchased from Origene (Cat. … RC202367 and Cat. … RC202054). Lenti-EZH1-GFP and Lenti-EZH2-GFP were constructed based on these cDNA clones using a GFP-expressing lenti-vector as we previously described (Zhang e;EZH1 and EZH2 cDNA clones were purchased from Origene (Cat. … RC202367 and Cat. … RC202054)
Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia
Dou, Yan, Pang et al
Nat Commun (2019) 10 (1), 5051 DOI: 10.1038/s41467-019-12960-6
Dou, Yan, Pang et al
Nat Commun (2019) 10 (1), 5051 DOI: 10.1038/s41467-019-12960-6
AI Summary: In this study, OriGene products such as EZH1 shRNA (OriGene, #TL313082) and AML1-ETO expression plasmids (OriGene, #RC209605) were used to manipulate gene expression and investigate the role of EZH1-mediated lysine 43 methylation in AML1-ETO-driven transcriptional repression in leukemia.
Snippet: tivities. Although observed less frequently, lysine methylation of non-histone proteins (e.g., p5310, TAF1011, RORA12, GATA413, STAT314) critically regulates their functions, and is essential for tumorigenesis15. AML1-ETO is a leukemia-initiating transcriptional factor that has many lysine residues. Yet whether and how AML1-ETO is subjected to regulation by lysine methylation is unclear. It is po
Showing 1-2 of 2 papers.
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