RNPC3 (NM_017619) Human Tagged ORF Clone

SKU RC202319

RNPC3 (Myc-DDK-tagged)-Human RNA-binding region (RNP1, RRM) containing 3 (RNPC3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RNPC3
• Synonyms: IGHD5; RBM40; RNP; SNRNP65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202319 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGCTCCCGAGCAGCCGCTTGCGATATCAAGGGGATGCACGAGCTCCTCCTCGCTTTCCCCGCCTC; GGGGCGACCGAACCCTTCTGGTCAGGCACCTGCCGGCTGAGCTTACTGCTGAGGAGAAAGAGGACTTGCT; GAAGTACTTCGGGGCTCAGTCTGTGCGGGTCCTGTCAGATAAGGGGCGACTGAAACATACAGCTTTTGCC; ACATTCCCTAATGAAAAAGCAGCTATAAAGGCATTGACAAGACTCCATCAACTGAAACTTTTAGGTCATA; CTTTAGTCGTTGAATTTGCAAAAGAGCAAGATCGAGTTCACTCCCCATGTCCCACTTCAGGCTCTGAAAA; AAAAAAAAGGTCTGATGACCCTGTCGAAGATGATAAAGAAAAAAAAGAACTTGGTTATTTAACAGTAGAA; AATGGAATTGCACCAAACCATGGGCTGACTTTTCCTTTAAATTCATGCCTCAAGTATATGTACCCACCAC; CTTCCAGCACAATCCTAGCAAACATTGTAAATGCCTTGGCAAGCGTGCCTAAGTTCTATGTACAGGTCCT; TCATCTTATGAATAAAATGAATTTGCCCACACCTTTTGGACCAATTACTGCGCGACCTCCCATGTATGAA; GACTATATGCCATTGCATGCACCTCTTCCACCCACATCTCCTCAGCCACCTGAGGAACCTCCTTTGCCAG; ACGAGGATGAGGAATTATCTAGTGAAGAATCAGAATATGAAAGCACTGATGATGAGGACCGACAGAGAAT; GAACAAATTAATGGAACTAGCAAATCTTCAGCCCAAAAGACCTAAAACAATAAAGCAGCGCCATGTGAGA; AAAAAGAGAAAAATAAAGGATATGTTGAATACACCTTTGTGTCCTTCACACAGCAGTTTACATCCAGTGC; TGTTACCTTCAGATGTATTTGACCAACCACAACCTGTAGGTAACAAAAGAATTGAATTCCATATATCTAC; CGACATGCCAGCTGCATTTAAGAAAGATTTAGAAAAGGAACAAAATTGTGAGGAAAAAAATCATGATTTA; CCTGCTACTGAAGTTGATGCATCCAATATAGGATTTGGAAAAATCTTCCCCAAACCTAATTTGGACATCA; CAGAGGAGATTAAAGAAGACTCTGATGAAATGCCTTCAGAATGTATTTCTAGAAGGGAATTGGAAAAGGG; CAGAATTTCTAGAGAAGAAATGGAAACACTTTCAGTTTTCAGAAGTTATGAACCGGGTGAACCAAACTGT; AGAATTTATGTAAAGAATTTAGCTAAACATGTTCAAGAAAAGGACCTTAAATATATTTTTGGAAGATATG; TTGACTTTTCATCAGAAACACAGCGGATCATGTTTGATATACGTTTGATGAAAGAAGGTCGTATGAAAGG; ACAAGCTTTCATTGGACTTCCTAATGAAAAAGCAGCAGCAAAAGCCTTAAAGGAAGCTAATGGATATGTG; CTTTTTGGAAAACCCATGGTGGTTCAGTTTGCTCGATCTGCTAGACCAAAACAAGATCCTAAGGAAGGAA; AAAGAAAGTGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202319 protein sequence; Red=Cloning site Green=Tags(s); ; MAAPEQPLAISRGCTSSSSLSPPRGDRTLLVRHLPAELTAEEKEDLLKYFGAQSVRVLSDKGRLKHTAFA; TFPNEKAAIKALTRLHQLKLLGHTLVVEFAKEQDRVHSPCPTSGSEKKKRSDDPVEDDKEKKELGYLTVE; NGIAPNHGLTFPLNSCLKYMYPPPSSTILANIVNALASVPKFYVQVLHLMNKMNLPTPFGPITARPPMYE; DYMPLHAPLPPTSPQPPEEPPLPDEDEELSSEESEYESTDDEDRQRMNKLMELANLQPKRPKTIKQRHVR; KKRKIKDMLNTPLCPSHSSLHPVLLPSDVFDQPQPVGNKRIEFHISTDMPAAFKKDLEKEQNCEEKNHDL; PATEVDASNIGFGKIFPKPNLDITEEIKEDSDEMPSECISRRELEKGRISREEMETLSVFRSYEPGEPNC; RIYVKNLAKHVQEKDLKYIFGRYVDFSSETQRIMFDIRLMKEGRMKGQAFIGLPNEKAAAKALKEANGYV; LFGKPMVVQFARSARPKQDPKEGKRKC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017619
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017619.4
• RefSeq Size: 1901 bp
• RefSeq ORF: 1554 bp
• Locus ID: 55599
• UniProt ID: Q96LT9
• Cytogenetics: 1p21.1
• MW: 58.6 kDa
• Summary: Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 65K protein that is a component of the U12-type spliceosome. This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome. [provided by RefSeq, Jul 2008]