RNPC3 (NM_017619) Human Untagged Clone

SKU SC121721

RNPC3 (untagged)-Human RNA-binding region (RNP1, RRM) containing 3 (RNPC3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RNPC3
• Synonyms: IGHD5; RBM40; RNP; SNRNP65
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121721 sequence for NM_017619 edited (data generated by NextGen Sequencing); ATGGCAGCTCCCGAGCAGCCGCTTGCGATATCAAGGGGATGCACGAGCTCCTCCTCGCTT; TCCCCGCCTCGGGGCGACCGAACCCTTCTGGTCAGGCACCTGCCGGCTGAGCTTACTGCT; GAGGAGAAAGAGGACTTGCTGAAGTACTTCGGGGCTCAGTCTGTGCGGGTCCTGTCAGAT; AAGGGGCGACTGAAACATACAGCTTTTGCCACATTCCCTAATGAAAAAGCAGCTATAAAG; GCATTGACAAGACTCCATCAACTGAAACTTTTAGGTCATACTTTAGTCGTTGAATTTGCA; AAAGAGCAAGATCGAGTTCACTCCCCATGTCCCACTTCAGGCTCTGAAAAAAAAAAAAGG; TCTGATGACCCTGTCGAAGATGATAAAGAAAAAAAAGAACTTGGTTATTTAACAGTAGAA; AATGGAATTGCACCAAACCATGGGCTGACTTTTCCTTTAAATTCATGCCTCAAGTATATG; TACCCACCACCTTCCAGCACAATCCTAGCAAACATTGTAAATGCCTTGGCAAGCGTGCCT; AAGTTCTATGTACAGGTCCTTCATCTTATGAATAAAATGAATTTGCCCACACCTTTTGGA; CCAATTACTGCGCGACCTCCCATGTATGAAGACTATATGCCATTGCATGCACCTCTTCCA; CCCACATCTCCTCAGCCACCTGAGGAACCTCCTTTGCCAGACGAGGATGAGGAATTATCT; AGTGAAGAATCAGAATATGAAAGCACTGATGATGAGGACCGACAGAGAATGAACAAATTA; ATGGAACTAGCAAATCTTCAGCCCAAAAGACCTAAAACAATAAAGCAGCGCCATGTGAGA; AAAAAGAGAAAAATAAAGGATATGTTGAATACACCTTTGTGTCCTTCACACAGCAGTTTA; CATCCAGTGCTGTTACCTTCAGATGTATTTGACCAACCACAACCTGTAGGTAACAAAAGA; ATTGAATTCCATATATCTACCGACATGCCAGCTGCATTTAAGAAAGATTTAGAAAAGGAA; CAAAATTGTGAGGAAAAAAATCATGATTTACCTGCTACTGAAGTTGATGCATCCAATATA; GGATTTGGAAAAATCTTCCCCAAACCTAATTTGGACATCACAGAGGAGATTAAAGAAGAC; TCTGATGAAATGCCTTCAGAATGTATTTCTAGAAGGGAATTGGAAAAGGGCAGAATTTCT; AGAGAAGAAATGGAAACACTTTCAGTTTTCAGAAGTTATGAACCGGGTGAACCAAACTGT; AGAATTTATGTAAAGAATTTAGCTAAACATGTTCAAGAAAAGGACCTTAAATATATTTTT; GGAAGATATGTTGACTTTTCATCAGAAACACAGCGGATCATGTTTGATATACGTTTGATG; AAAGAAGGTCGTATGAAAGGACAAGCTTTCATTGGACTTCCTAATGAAAAAGCAGCAGCA; AAAGCCTTAAAGGAAGCTAATGGATATGTGCTTTTTGGAAAACCCATGGTGGTTCAGTTT; GCTCGATCTGCTAGACCAAAACAAGATCCTAAGGAAGGAAAAAGAAAGTGTTAA; ; Clone variation with respect to NM_017619.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017619 unedited; CGCGAGGCGGAAAAAATATTTCTCCCAGCTTGTGTTGATGCCGCGATTTTGACTGAGACT; TCTTCCCACGATTTCTGTTTTTGCTTCTCCAAGGAAAATGGCAGCTCCCGAGCAGCCGCT; TGCGATATCAAGGGGATGCACGAGCTCCTCCTCGCTTTCCCCGCCTCGGGGCGACCGAAC; CCTTCTGGTCAGGCACCTGCCGGCTGAGCTTACTGCTGAGGAGAAAGAGGACTTGCTGAA; GTACTTCGGGGCTCAGTCTGTGCGGGTCCTGTCAGATAAGGGGCGACTGAAACATACAGC; TTTTGCCACATTCCCTAATGAAAAAGCAGCTATAAAGGCATTGACAAGACTCCATCAACT; GAAACTTTTAGGTCATACTTTAGTCGTTGAATTTGCAAAAGAGCAAGATCGAGTTCACTC; CCCATGTCCCACTTCAGGCTCTGAAAAAAAAAAAAGGTCTGATGACCCTGTCGAAGATGA; TAAAGAAAAAAAAGAACTTGGTTATTTAACAGTAGAAAATGGAATTGCACCAAACCATGG; GCTGACTTTTCCTTTAAATTCATGCCTCAAGTATATGTACCCACCACCTTCCAGCACAAT; CCTAGCAAAACATTGTAAATGCCTTGGCAAGCGGGCCTAAGTTCTATGGTACAGGTCCTT; CATCTTATGAATAAAATGAATTTGCCCACACCTTTTGGACCAATTACTGCGCGACCTNCC; ATGGTATGAAGACTATATGCCATTGCATGCACCTNTTTCACCCACATCTTCTCAGCNCCT; GAGGAACCTCNTTGNCAACCAGGGTTGAGGATTTTTTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017619
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017619.3, NP_060089.1
• RefSeq Size: 1901 bp
• RefSeq ORF: 1554 bp
• Locus ID: 55599
• UniProt ID: Q96LT9
• Cytogenetics: 1p21.1
• Summary: Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 65K protein that is a component of the U12-type spliceosome. This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome. [provided by RefSeq, Jul 2008]