BRF2 (NM_018310) Human Tagged ORF Clone

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SKU
RC202314
BRF2 (Myc-DDK-tagged)-Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BRF2
Synonyms BRFU; TFIIIB50
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202314 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACGGAGCTGGTGGAAGACTCGCACTATTCGCAGA
GCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAGGGGGTCCTTACCACTACCTTCAGCGACGA
GGGCAATCTCCGAGAGGTAACATATTCCCGAAGCACAGGGGAAAACGAACAAGTTAGTCGCAGCCAGCAA
CGAGGTCTCCGGCGAGTGAGAGACCTTTGTCGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCGG
TTGCCTACTACCAACAGGCATATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAGAAGGAGGTGTT
GGTTGGGTGCTGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTAACAATGGGGGCCATCTGCACG
CTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTACATGCAGATAGTGAAGCTCCTGGGACTGGATG
TGCCATCTCTGTGCTTGGCAGAACTGGTGAAGACCTATTGCAGCAGCTTCAAACTGTTCCAAGCTTCACC
TTCTGTGCCAGCCAAATACGTGGAAGACAAAGAGAAGATGCTGTCTCGAACAATGCAGTTGGTGGAGCTG
GCAAATGAGACGTGGCTGGTGACCGGGAGGCATCCCTTGCCCGTCATCACTGCTGCGACTTTCCTGGCTT
GGCAGTCGCTGCAGCCTGCAGATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAAATTGGCAAATGTGGA
CCTGCCCTACCCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGTGCTGCTGCGGATGGCTGAGCAGCTG
GCCTGGTTACGAGTTCTGAGACTTGACAAACGGTCTGTGGTGAAGCACATCGGTGACCTTCTCCAGCACC
GCCAGTCACTGGTCCGCTCTGCCTTTCGGGATGGGACAGCAGAAGTGGAGACCCGAGAGAAGGAGCCACC
GGGGTGGGGACAGGGGCAAGGAGAAGGGGAGGTGGGAAATAATTCCTTAGGTTTACCCCAGGGGAAGCGG
CCGGCCAGTCCTGCCCTTCTCTTGCCACCCTGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGTACCCC
CTGTCTCCACTGTCACTGGAGATGAGAACATTTCTGATAGTGAAATAGAACAGTATTTGCGTACCCCTCA
GGAAGTTAGGGACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGCCACGAGTGTCCCTAACCCTCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202314 protein sequence
Red=Cloning site Green=Tags(s)

MPGRGRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTFSDEGNLREVTYSRSTGENEQVSRSQQ
RGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYRHSGIRAARLQKKEVLVGCCVLITCRQHNWPLTMGAICT
LLYADLDVFSSTYMQIVKLLGLDVPSLCLAELVKTYCSSFKLFQASPSVPAKYVEDKEKMLSRTMQLVEL
ANETWLVTGRHPLPVITAATFLAWQSLQPADRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQL
AWLRVLRLDKRSVVKHIGDLLQHRQSLVRSAFRDGTAEVETREKEPPGWGQGQGEGEVGNNSLGLPQGKR
PASPALLLPPCMLKSPKRICPVPPVSTVTGDENISDSEIEQYLRTPQEVRDFQRAQAARQAATSVPNPP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018310
ORF Size 1257 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018310.4
RefSeq Size 2007 bp
RefSeq ORF 1260 bp
Locus ID 55290
UniProt ID Q9HAW0
Cytogenetics 8p11.23
Protein Families Druggable Genome
MW 46.5 kDa
Summary This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_018310" in other vectors (4)
SKU Description Size Price
RC202314L3 Lenti ORF clone of Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), Myc-DDK-tagged 10 ug
$757.00
RC202314L4 Lenti ORF clone of Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), mGFP tagged 10 ug
$757.00
RG202314 BRF2 (tGFP-tagged) - Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2) 10 ug
$489.00 $657.00
SC113533 BRF2 (untagged)-Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2) 10 ug
$457.00

Plasmid Map for RC202314

Download sequence for

Cloning scheme for RC202314

Circular map for RC202314

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