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MED30 (NM_080651) Human Tagged ORF Clone

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SKU
RC202305
MED30 (Myc-DDK-tagged)-Human mediator complex subunit 30 (MED30)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MED30
Synonyms MED30S; THRAP6; TRAP25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202305 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACCCCTCCGTTGGCCGCGTCGGGGATGGCGCCCGGGCCCTTCGCCGGGCCCCAGGCTCAGCAGG
CCGCCCGGGAAGTCAACACGGCGTCGCTGTGCCGCATCGGGCAGGAGACAGTGCAGGACATCGTGTACCG
CACCATGGAGATCTTCCAGCTCCTGAGGAACATGCAGCTGCCAAATGGTGTCACTTACCACACTGGAACA
TATCAAGACCGGTTAACAAAGCTACAGGATAATCTTCGCCAACTTTCAGTTCTCTTCAGGAAGCTGAGAT
TGGTATATGACAAATGCAATGAAAACTGTGGTGGGATGGATCCCATTCCAGTCGAGCAACTTATTCCATA
TGTGGAAGAAGATGGCTCAAAGAATGATGATCGGGCTGGCCCACCTCGTTTTGCTAGTGAAGAGAGGCGA
GAAATTGCTGAAGTAAATAAAAAACTCAAACAGAAGAATCAACAGCTGAAACAAATTATGGATCAATTAC
GAAATCTCATCTGGGATATAAATGCCATGTTGGCAATGAGGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202305 protein sequence
Red=Cloning site Green=Tags(s)

MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQLPNGVTYHTGT
YQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERR
EIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080651
ORF Size 534 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080651.4
RefSeq Size 994 bp
RefSeq ORF 537 bp
Locus ID 90390
UniProt ID Q96HR3
Cytogenetics 8q24.11
Protein Families Druggable Genome, Transcription Factors
MW 20.3 kDa
Summary The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 PubMed 11909976).supplied by OMIM, Nov 2010
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Documents
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"NM_080651" in other vectors (4)
SKU Description Size Price
RC202305L3 Lenti ORF clone of Human mediator complex subunit 30 (MED30), Myc-DDK-tagged 10 ug
$600.00
RC202305L4 Lenti ORF clone of Human mediator complex subunit 30 (MED30), mGFP tagged 10 ug
$600.00
RG202305 MED30 (tGFP-tagged) - Human mediator complex subunit 30 (MED30) 10 ug
$489.00 $500.00
SC322247 MED30 (untagged)-Human mediator complex subunit 30 (MED30) 10 ug
$300.00

Plasmid Map for RC202305

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202305
Circular map for RC202305

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