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EFCAB7 (NM_032437) Human Tagged ORF Clone

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SKU
RC202261
EFCAB7 (Myc-DDK-tagged)-Human EF-hand calcium binding domain 7 (EFCAB7)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $585.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EFCAB7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202261 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGATCAGTCCACGAAGCGATGCAACTTTCTCCAGTCAGAAATCAACACCTTCAGAGAGTCCTCGAA
CAAAGAAATTTCCACTAACTGAAGAGGAAATATTTTATATGAATTGTAGAGCTGCCTACTTAACTGTCTT
CAAAAGCAGCTTGGAAAACATTATTTCTAAAGATCAACTTTACTTAGCTCTTCAGCATGCAGGAAGAAAT
CCATCCCAAAAGACCATTAATAAGTATTGGACTCCTCAAACTGCCAAACTGAATTTTGATGATTTTTGTA
TAATTTTAAGGAAGGAAAAACCTACTTCAAAAGCAGAACTACTAAAATCTTTTAAGCAATTAGATGTAAA
TGATGATGGCTGTATTTTACACACTGACCTTTATAAATTTCTAACAAAGAGAGGTGAGAAGATGACTCGA
GAAGAAGTAAATGCCATAATAAATTTGGCTGATGTAAATGCTGATGGCAAATTTGACTACATCAAGTTTT
GTAAATTATATATGACAACCAACGAGCAATGTCTCAAGACTACACTAGAAAAACTAGAGGTTGACAGTAA
ATTGATGCGTCACCAGTTTGGAAACCACATCGAAGGGTCCCCTGAAAGGGACCCATCACCAGTACCAAAA
CCATCACCTAAAATCACAAGAAAAACTGATCCAGAAACATTCTTAAATAAAGGTGACACCAGGAGTTCTT
TACTGTCAGCAACCAGGAAGTTCAAAACATCTGTTTCCTTCATAGTTACCATGGGGGCTAATGGTAACCG
AAACTCAAAGTTAACGGAGCCAAATTTAATAAAGGACTGGCAACACATGCAATCAAAAGGTTGCTTCTTC
TTAGAAGAAGATGGTGAAATCATTAGTCATCAGTACAGGATGCAAATAGCTCAGAGGTCCATGGTTTATC
TAACAATTAAGCCATTAAACCTGAGTCAAGTTGAAGGAAAACCATCCCCTTGGTTATCCGTTGATACTGC
CTTGTATATTCTCAAGGAAAATGAGAGTCAAGCAAATCTACAGCTTGTGTGTTTTACCGAACTACGAAAT
AGAGAAGTGTTTGGATGGACTGGTGAACTAGGACCTGGAATTTACTGGTTAATTCCTTCCACAACTGGCT
GTAAGCTGAGGAAAAAAATAAAACCAGTAACAGATGAAGCCCAACTTGTATATAGAGATGAAACAGGGGA
ATTATTCCTTACAAAGGAATTTAAGTCTACTTTATCAGATATATTTGAAGTAATTGATTTAGATGGAAAT
GGTCTTCTTAGCCTTGAAGAATATAATTTTTTTGAATTGAGAACAAGTGGTGAGAAATGTGATGAAGATG
CTTGGGCTGTCTGCAGAGAGAATTTTGATACAAAGAGGAATGAACTAACAAGACAAGGATTTATGGATTT
GAATCTAATGGAAGCTAATGATCGAGAAGGAGATCCTTGTGACCTTTGGGTAACTCTACACTCTATGGGC
TACAATAAAGCTCTGGAGTTGACAGAGGCATGTCCATTTGTCATTGATATCTATGCAGAAAAATGCAAGC
CAAAAATTAAAGCTGTCCATATGGAGGCATGTAGTGGACAACTTGAGAAGGCCATTTGTAAATCTGTTCT
TAGCAACGGTGATGCCAAAGTAATGGATGGCTATGAAAATATAATCGTGCATACTTACAGTTGTGACACC
TGGATAACGTCAGTTATTGAAAACAAGTCAGATGAGAAAGTGATTATTCACATCAGCAATGAGCTAAGTA
AAAACTGCATAAACAACAGAGGACTCAACATATTTGCAGTAGAAGTGGGACCCAAATCTACAATGGTTTG
TCAACATGTAATGCCTTTGAATGAACGACAAGAATGGATATATTATTGTATATATTCTCTTATTTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202261 protein sequence
Red=Cloning site Green=Tags(s)

MAISPRSDATFSSQKSTPSESPRTKKFPLTEEEIFYMNCRAAYLTVFKSSLENIISKDQLYLALQHAGRN
PSQKTINKYWTPQTAKLNFDDFCIILRKEKPTSKAELLKSFKQLDVNDDGCILHTDLYKFLTKRGEKMTR
EEVNAIINLADVNADGKFDYIKFCKLYMTTNEQCLKTTLEKLEVDSKLMRHQFGNHIEGSPERDPSPVPK
PSPKITRKTDPETFLNKGDTRSSLLSATRKFKTSVSFIVTMGANGNRNSKLTEPNLIKDWQHMQSKGCFF
LEEDGEIISHQYRMQIAQRSMVYLTIKPLNLSQVEGKPSPWLSVDTALYILKENESQANLQLVCFTELRN
REVFGWTGELGPGIYWLIPSTTGCKLRKKIKPVTDEAQLVYRDETGELFLTKEFKSTLSDIFEVIDLDGN
GLLSLEEYNFFELRTSGEKCDEDAWAVCRENFDTKRNELTRQGFMDLNLMEANDREGDPCDLWVTLHSMG
YNKALELTEACPFVIDIYAEKCKPKIKAVHMEACSGQLEKAICKSVLSNGDAKVMDGYENIIVHTYSCDT
WITSVIENKSDEKVIIHISNELSKNCINNRGLNIFAVEVGPKSTMVCQHVMPLNERQEWIYYCIYSLIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032437
ORF Size 1887 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032437.1, NP_115813.1
RefSeq Size 2376 bp
RefSeq ORF 1890 bp
Locus ID 84455
UniProt ID A8K855
Cytogenetics 1p31.3
MW 71.9 kDa
Summary Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling. Required for the localization of the EVC2:EVC subcomplex at the base of primary cilia.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_032437" in other vectors (4)
SKU Description Size Price
RC202261L3 Lenti ORF clone of Human EF-hand calcium binding domain 7 (EFCAB7), Myc-DDK-tagged 10 ug
$885.00
RC202261L4 Lenti ORF clone of Human EF-hand calcium binding domain 7 (EFCAB7), mGFP tagged 10 ug
$885.00
RG202261 EFCAB7 (tGFP-tagged) - Human EF-hand calcium binding domain 7 (EFCAB7) 10 ug
$785.00
SC305448 EFCAB7 (untagged)-Human EF-hand calcium binding domain 7 (EFCAB7) 10 ug
$645.00

Plasmid Map for RC202261

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202261
Circular map for RC202261

file-alt Citations

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