Mitofusin 2 (MFN2) (NM_014874) Human Tagged ORF Clone
SKU
RC202218
MFN2 (Myc-DDK-tagged)-Human mitofusin 2 (MFN2), nuclear gene encoding mitochondrial protein, transcript variant 1
$693.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Mitofusin 2 |
| Synonyms | CMT2A; CMT2A2; CMT2A2A; CMT2A2B; CPRP1; HMSN6A; HSG; MARF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014874 |
| ORF Size | 2271 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014874.4 |
| RefSeq Size | 4685 bp |
| RefSeq ORF | 2274 bp |
| Locus ID | 9927 |
| UniProt ID | O95140 |
| Cytogenetics | 1p36.22 |
| Domains | fzo_mitofusin |
| Protein Families | Transmembrane |
| MW | 86.4 kDa |
| Summary | This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014874" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202218L1 | Lenti ORF clone of Human mitofusin 2 (MFN2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged | 10 ug |
$993.00
|
|
| RC202218L2 | Lenti ORF clone of Human mitofusin 2 (MFN2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged | 10 ug |
$993.00
|
|
| RC202218L3 | Lenti ORF clone of Human mitofusin 2 (MFN2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged | 10 ug |
$993.00
|
|
| RC202218L4 | Lenti ORF clone of Human mitofusin 2 (MFN2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged | 10 ug |
$993.00
|
|
| RG202218 | MFN2 (tGFP-tagged) - Human mitofusin 2 (MFN2), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$789.00
$893.00
|
|
| SC114726 | MFN2 (untagged)-Human mitofusin 2 (MFN2), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$694.00
|
|
Citations
Combined RNA interference and gene replacement therapy targeting MFN2 as proof of principle for the treatment of Charcot-Marie-Tooth type 2A
Rizzo, Bono, Ruepp et al
Cell Mol Life Sci (2023) 80 (12), 373 DOI: 10.1007/s00018-023-05018-w
Rizzo, Bono, Ruepp et al
Cell Mol Life Sci (2023) 80 (12), 373 DOI: 10.1007/s00018-023-05018-w
Cited by 21
AI Summary: OriGene’s C-terminally Myc-DDK-tagged MFN2 plasmid (pCMV6-MFN2-Myc-DDK; #RC202218) was used in this study to overexpress a functional, RNAi-resistant MFN2 protein, enabling assessment of gene replacement therapy efficacy in models of Charcot-Marie-Tooth type 2A.
Snippet: 9 (5-GCAAAGCTGCTCAGGAATA-3′) of MFN2 mRNA (numbering according to NM_014874.4). pSUPuro-scrambled (5′-ATTCTCCGAACGTGTCACG-3) is described elsewhere [37]. C-terminally Myc-DDK-tagged MFN2 was from Origene (pCMV6-MFN2-Myc-DDK;#RC202218;Myc-tag: EQKLISEEDL ~ 1202 Daltons;FLAG-tag: DYKDDDDK ~ 1012 Da). To render the MFN2 cDNA RNAi-resistant, silent mutations were introduced into the
Combined RNA interference and gene replacement therapy targeting MFN2 for the treatment of Charcot-Marie-Tooth type 2A
Rizzo, Bono, Ruepp et al
Research Square (2023)
Rizzo, Bono, Ruepp et al
Research Square (2023)
Snippet: Mitofusin-2 (MFN2) is an outer mitochondrial membrane protein essential for mitochondrial networking in most cells. Autosomal dominant mutations in the MFN2 gene Save;shRNAtg1 and pSUPuro-MFN2-shRNAtg2 target nucleotides 1564-1582 (5’-CCTCAAGGTTTATAAGAAT-3’) and 2381-2399 (5-GCAAAGCTGCTCAGGAATA-3’) of MFN2 mRNA (numbering according to NM_014874.4). … C-terminally Myc-DDK-tagged MFN2 was from Origene (pCMV6-MFN2-Myc-DDK;#RC202218;Myc-tag: EQKLISEEDL ~1202 Daltons;FLAG-tag: DYKDDDDK ~1012 Da)
SUMOylation of mitofusins: a potential mechanism for perinuclear mitochondrial congression in cells treated with mitochondrial stressors
Kim, Juncker, Reed et al
Biochim Biophys Acta Mol Basis Dis (2021) 1867 (6), 166104 DOI: 10.1016/j.bbadis.2021.166104
Kim, Juncker, Reed et al
Biochim Biophys Acta Mol Basis Dis (2021) 1867 (6), 166104 DOI: 10.1016/j.bbadis.2021.166104
Cited by 17
Showing 1-3 of 3 papers.
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