KEAP1 (NM_203500) Human Tagged ORF Clone
SKU
RC202189
KEAP1 (Myc-DDK-tagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1
$871.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | KEAP1 |
| Synonyms | INrf2; KLHL19 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_203500 |
| ORF Size | 1872 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_203500.2 |
| RefSeq Size | 2606 bp |
| RefSeq ORF | 1875 bp |
| Locus ID | 9817 |
| UniProt ID | Q14145, A0A024R7C0 |
| Cytogenetics | 19p13.2 |
| Protein Families | Transcription Factors |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 69.7 kDa |
| Summary | This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_203500" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202189L3 | Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,171.00
|
|
| RC202189L4 | Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1, mGFP tagged | 10 ug |
$1,171.00
|
|
| RG202189 | KEAP1 (tGFP-tagged) - Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1 | 10 ug |
$1,071.00
|
|
| SC324644 | KEAP1 (untagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1 | 10 ug |
$582.00
|
|
Citations
Chemoproteomic Profiling of Clickable Fumarate Probes for Target Identification and Mechanism of Action Studies
Zhang, Martin, Gao et al
ACS Chem Biol (2025) 20 (2), 340-356 DOI: 10.1021/acschembio.4c00617
Zhang, Martin, Gao et al
ACS Chem Biol (2025) 20 (2), 340-356 DOI: 10.1021/acschembio.4c00617
Cited by 5
AI Summary: OriGene products contributed to this paper by providing plasmids for human KEAP1 (CAT#: RC202189) and T-Plastin (PLS3) (CAT#: RC208934), which were used for target validation in the chemoproteomic profiling studies.
Snippet: ll other chemicals were purchased from Sigma-Aldrich. Streptavidin agarose resin was purchased from Thermo Fisher Scientific (20353). Dimethyl fumarate (242926), monomethyl fumarate (651419), and thiophenol (240249) were purchased from Sigma-Aldrich. Plasmids were sourced from OriGene, including human KEAP1 (CAT#: RC202189) and T-Plastin (PLS3) (CAT#: RC208934). Synthesis
SFTS virus NSs interacts with TRIM21 to activate the p62-Keap1-Nrf2 pathway
Choi, Jiang, Shin et al
J. Virol. (2019)
Choi, Jiang, Shin et al
J. Virol. (2019)
AI Summary: OriGene products, specifically the cDNAs for human TRIM21, p62, Keap1, ABIN2, and TBK1, were used in this study to generate expression constructs that enabled the investigation of how SFTSV NSs protein interacts with TRIM21 to activate the p62-Keap1-Nrf2 antioxidant pathway.
Snippet: … cDNAs for human TRIM21, p62, Keap1, ABIN2, and TBK1 were obtained from Addgene 348 and Origene. … All NSs-expression plasmids contain a C-terminal 3xFlag, V5, or 3xFlag/GFP 351 tags and N-terminal GST tag, TRIM21-expression plasmid has a C-terminal V5 tag, p62- 352 expression plasmids have C-terminal Myc or HA tags, Keap1-expression plasmid contains a C353 terminal Flag tag, ABIN2-expression plasmid contains a C-terminal V5 tag, and TBK1- 354 expression plasmid contains a C-terminal Flag tag. …
Showing 1-2 of 2 papers.
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