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PPIP5K2 (NM_015216) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC202100
PPIP5K2 (Myc-DDK-tagged)-Human diphosphoinositol pentakisphosphate kinase 2 (PPIP5K2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,064.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPIP5K2
Synonyms CFAP160; DFNB100; HISPPD1; IP7K2; VIP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202100 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAAGCCCCCAGATTCTTCGTTGGACCAGAAGATACAGAAATAAATCCTGGAAATTATCGACATT
TCTTCCACCATGCAGATGAAGACGATGAGGAGGAAGATGATTCTCCACCAGAAAGGCAGATTGTGGTTGG
AATATGTTCCATGGCAAAGAAATCCAAATCCAAACCAATGAAGGAAATTCTTGAACGGATCTCCTTATTT
AAATATATCACAGTAGTAGTATTTGAAGAGGAGGTTATTTTGAATGAACCAGTGGAAAACTGGCCTTTAT
GTGATTGTCTTATTTCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCGGTTGCCTATGCAAAACTCAG
GAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGGAGAGAAGTATATAGTATT
CTTCAAGCTGAAGGTATTTTACTTCCTCGTTATGCTATTTTGAACCGTGACCCAAATAATCCCAAAGAAT
GTAATCTGATTGAAGGGGAAGATCATGTAGAAGTAAATGGGGAAGTTTTTCAAAAGCCATTTGTAGAAAA
GCCAGTCAGTGCAGAAGATCACAATGTTTACATTTATTACCCAACTTCTGCTGGTGGTGGAAGTCAAAGA
CTCTTTAGAAAGATTGGCAGTAGAAGTAGTGTTTATTCTCCAGAAAGCAATGTACGAAAAACAGGCTCAT
ATATATATGAAGAGTTTATGCCCACAGATGGTACTGATGTTAAGGTTTATACAGTGGGTCCAGATTATGC
CCATGCTGAAGCTCGAAAATCTCCAGCACTTGATGGCAAGGTGGAACGAGACAGTGAAGGAAAAGAAGTA
AGATACCCTGTTATTCTCAATGCACGAGAGAAATTAATTGCTTGGAAAGTCTGCCTTGCTTTTAAGCAAA
CAGTTTGTGGCTTTGATTTGTTACGGGCCAATGGACAGTCCTATGTCTGTGATGTCAATGGCTTCAGTTT
TGTGAAAAATTCCATGAAGTATTATGATGACTGTGCAAAAATACTTGGAAATATTGTAATGCGAGAACTT
GCTCCACAATTTCATATTCCATGGTCAATACCCTTAGAAGCTGAAGATATCCCAATTGTACCAACTACAT
CTGGAACTATGATGGAACTTAGATGTGTCATAGCTGTTATACGTCATGGGGATCGAACACCAAAACAAAA
AATGAAAATGGAAGTGAGACATCAGAAATTTTTTGATCTTTTTGAAAAGTGTGATGGATATAAATCAGGG
AAATTAAAACTCAAAAAACCAAAACAGTTACAGGAAGTGCTAGATATTGCACGACAGCTTCTTATGGAGC
TAGGGCAAAATAATGATTCTGAAATTGAAGAAAACAAGCCAAAACTTGAACAACTTAAGACTGTATTAGA
GATGTATGGTCATTTTTCTGGAATAAATCGTAAGGTTCAGTTGACCTATCTCCCTCATGGTTGTCCTAAA
ACATCTAGTGAAGAGGAGGACAGCCGAAGAGAAGAACCATCTTTACTTTTGGTTCTAAAATGGGGAGGTG
AATTAACTCCTGCAGGCAGGGTCCAGGCTGAAGAACTTGGAAGAGCCTTCAGGTGTATGTATCCTGGAGG
TCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTTAGATTACATAGCACCTACAGACATGACCTC
AAAATATATGCCTCTGATGAAGGACGAGTCCAGATGACTGCAGCTGCTTTTGCAAAGGGGCTTTTAGCTT
TGGAAGGAGAGCTTACACCCATTCTTGTTCAAATGGTGAAAAGTGCAAATATGAACGGTCTTTTGGATAG
TGATAGTGACTCTCTGAGCAGTTGTCAGCAACGTGTGAAGGCAAGGCTTCATGAAATACTTCAGAAAGAC
AGAGATTTTACTGCTGAAGATTATGAAAAGCTTACTCCATCTGGAAGCATTTCTCTTATCAAATCAATGC
ATTTAATTAAAAACCCTGTGAAGACCTGTGATAAAGTTTATTCCTTAATTCAGAGTTTGACTTCTCAAAT
CAGACATCGAATGGAAGATCCTAAATCATCAGATATTCAGCTTTACCATAGTGAAACATTGGAGCTTATG
CTACGTAGATGGTCCAAGTTAGAGAAAGACTTTAAAACAAAGAATGGAAGATATGATATTAGTAAAATCC
CTGACATATATGACTGTATAAAATATGATGTCCAGCATAATGGTTCCTTGAAATTAGAAAACACAATGGA
ATTATATAGGCTTTCGAAGGCATTAGCAGATATTGTTATCCCTCAGGAATATGGTATAACTAAAGCTGAA
AAACTGGAGATTGCCAAAGGCTACTGTACTCCTCTGGTTAGAAAAATTCGCTCAGACCTTCAGAGGACAC
AAGATGATGACACTGTAAATAAACTTCATCCTGTGTATTCTAGAGGTGTTCTGTCTCCTGAACGTCATGT
TCGTACTAGATTATATTTTACCAGTGAAAGTCATGTACATTCTTTGCTGTCTATTCTTCGCTATGGTGCC
TTATGCAATGAATCAAAGGATGAACAGTGGAAACGAGCTATGGATTATTTAAACGTTGTCAATGAGCTCA
ACTACATGACTCAGATTGTTATCATGCTTTATGAGGATCCTAATAAGGATCTTTCCTCAGAAGAACGCTT
TCATGTTGAATTACACTTTAGTCCGGGAGCCAAAGGTTGTGAAGAAGACAAAAATTTGCCATCTGGCTAT
GGATATAGACCAGCTTCCAGAGAGAATGAAGGCAGGAGACCTTTTAAAATTGATAATGATGATGAACCAC
ATACTTCTAAAAGAGATGAAGTTGATCGAGCTGTGATATTGTTTAAACCAATGGTATCAGAGCCAATTCA
TATACACAGGAAGTCTCCACTTCCAAGATCTAGGAAGACGGCTACAAATGATGAAGAGAGCCCCCTGAGT
GTGTCTAGCCCAGAGGGTACTGGTACCTGGCTGCATTATACCAGTGGTGTGGGTACTGGGCGTCGAAGAC
GCAGATCAGGGGAACAAATCACTTCTTCCCCTGTCTCCCCCAAATCATTGGCTTTCACATCCAGTATTTT
TGGCTCATGGCAACAGGTTGTATCTGAAAATGCTAATTACCTGCGAACACCAAGAACTCTTGTGGAACAG
AAGCAGAATCCTACTGTAGGGTCTCACTGTGCGGGCCTGTTTAGCACCTCGGTGCTCGGGGGTTCTTCAA
GCGCACCTAACCTACAGGATTATGCTCGTACTCATCGTAAAAAGCTGACCTCTTCTGGCTGCATAGATGG
CTTTGAATTGTATTCCATGGTGCCATCTATTTGTCCTCTAGAAACTCTTCATAATGCCCTATCTTTAAAG
CAAGTGGATGAATTTCTTGCTTCCATTGCTTCTCCATCATCTGACGTTCCACGGAAGACCGCTGAAATTT
CCTCTACAGCTTTACGTTCCAGTCCAATAATGAGAAAAAAAGTATCTTTAAATACGTATACACCTGCAAA
GATCCTCCCAACACCACCAGCTACCTTAAAGAGCACTAAAGCAAGCAGCAAACCAGCTACAAGTGGACCT
TCTAGTGCAGTTGTTCCTAATACCTCATCTCGGAAAAAGAATATAACTAGCAAAACAGAAACGCATGAAC
ACAAAAAAAACACTGGGAAAAAGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202100 protein sequence
Red=Cloning site Green=Tags(s)

MSEAPRFFVGPEDTEINPGNYRHFFHHADEDDEEEDDSPPERQIVVGICSMAKKSKSKPMKEILERISLF
KYITVVVFEEEVILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRREVYSI
LQAEGILLPRYAILNRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQR
LFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEV
RYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMREL
APQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSG
KLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKPKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPK
TSSEEEDSRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDL
KIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQKD
RDFTAEDYEKLTPSGSISLIKSMHLIKNPVKTCDKVYSLIQSLTSQIRHRMEDPKSSDIQLYHSETLELM
LRRWSKLEKDFKTKNGRYDISKIPDIYDCIKYDVQHNGSLKLENTMELYRLSKALADIVIPQEYGITKAE
KLEIAKGYCTPLVRKIRSDLQRTQDDDTVNKLHPVYSRGVLSPERHVRTRLYFTSESHVHSLLSILRYGA
LCNESKDEQWKRAMDYLNVVNELNYMTQIVIMLYEDPNKDLSSEERFHVELHFSPGAKGCEEDKNLPSGY
GYRPASRENEGRRPFKIDNDDEPHTSKRDEVDRAVILFKPMVSEPIHIHRKSPLPRSRKTATNDEESPLS
VSSPEGTGTWLHYTSGVGTGRRRRRSGEQITSSPVSPKSLAFTSSIFGSWQQVVSENANYLRTPRTLVEQ
KQNPTVGSHCAGLFSTSVLGGSSSAPNLQDYARTHRKKLTSSGCIDGFELYSMVPSICPLETLHNALSLK
QVDEFLASIASPSSDVPRKTAEISSTALRSSPIMRKKVSLNTYTPAKILPTPPATLKSTKASSKPATSGP
SSAVVPNTSSRKKNITSKTETHEHKKNTGKKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015216
ORF Size 3666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015216.2, NP_056031.2
RefSeq Size 5842 bp
RefSeq ORF 3669 bp
Locus ID 23262
UniProt ID O43314
Cytogenetics 5q21.1
MW 138.1 kDa
Summary This gene encodes a member of the histidine acid phosphatase family of proteins. Despite containing a histidine acid phosphatase domain, the encoded protein functions as an inositol pyrophosphate kinase, and is thought to lack phosphatase activity. This kinase activity is the mechanism by which the encoded protein synthesizes high-energy inositol pyrophosphates, which act as signaling molecules that regulate cellular homeostasis and other processes. This gene may be associated with autism spectrum disorder in human patients. provided by RefSeq, Sep 2016
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"NM_015216" in other vectors (4)
SKU Description Size Price
RC202100L3 Lenti ORF clone of Human diphosphoinositol pentakisphosphate kinase 2 (PPIP5K2), Myc-DDK-tagged 10 ug
$1,364.00
RC202100L4 Lenti ORF clone of Human diphosphoinositol pentakisphosphate kinase 2 (PPIP5K2), mGFP tagged 10 ug
$1,364.00
RG202100 PPIP5K2 (tGFP-tagged) - Human diphosphoinositol pentakisphosphate kinase 2 (PPIP5K2) 10 ug
$1,264.00
SC103513 PPIP5K2 (untagged)-Human diphosphoinositol pentakisphosphate kinase 2 (PPIP5K2) 10 ug
$1,065.00

Plasmid Map for RC202100

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202100
Circular map for RC202100

file-alt Citations

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