PEX10 (NM_002617) Human Tagged ORF Clone

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SKU
RC202036
PEX10 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 10 (PEX10), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PEX10
Synonyms NALD; PBD6A; PBD6B; RNF69
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202036 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCGGCCGCCGCCAGCCCCCCGGAGGTGATCCGCGCGGCGCAGAAGGACGAGTACTACCGCGGTG
GGCTGCGGAGCGCGGCGGGCGGCGCCCTGCACAGCCTGGCGGGTGCGAGGAAGTGGCTGGAGTGGAGGAA
GGAGGTTGAGCTGCTCTCAGATGTGGCCTACTTTGGCCTCACCACACTTGCAGGCTACCAGACCCTGGGG
GAGGAGTACGTCAGCATCATCCAGGTGGACCCATCGCGGATACATGTGCCCTCCTCGCTGCGCCGTGGCG
TGCTGGTGACACTGCATGCCGTCCTGCCCTACCTGCTGGACAAGGCCCTGCTCCCCCTGGAGCAGGAGCT
GCAGGCTGACCCCGACAGTGGGCGACCCTTGCAGGGGAGCCTGGGGCCAGGTGGGCGTGGCTGCTCAGGG
GCGCGGCGCTGGATGCGTCACCACACGGCCACCCTGACTGAGCAGCAGAGGAGGGCGCTGCTGCGGGCGG
TCTTCGTCCTCAGACAGGGCCTCGCCTGCCTCCAGCGGCTACATGTTGCCTGGTTTTACATCCACGGTGT
CTTCTACCACCTGGCCAAGAGGCTCACGGGGATCACGTACCTCCGTGTCCGCAGCCTGCCCGGAGAGGAC
CTGAGGGCCCGTGTTAGCTACAGGCTGCTGGGGGTCATCTCACTGCTGCACCTGGTGCTGTCCATGGGGC
TGCAGCTGTACGGTTTCAGGCAGCGGCAGCGAGCCAGGAAGGAGTGGAGGCTGCACCGCGGCCTGTCTCA
CCGCAGGGCCTCCTTGGAGGAGAGAGCCGTTTCCAGAAACCCCCTGTGCACCCTGTGCCTGGAGGAGCGC
AGGCACCCAACAGCCACGCCCTGCGGCCACCTGTTCTGCTGGGAGTGCATCACCGCGTGGTGCAGCAGCA
AGGCGGAGTGTCCCCTCTGCCGGGAGAAGTTCCCTCCCCAGAAGCTCATCTACCTTCGGCACTACCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202036 protein sequence
Red=Cloning site Green=Tags(s)

MAPAAASPPEVIRAAQKDEYYRGGLRSAAGGALHSLAGARKWLEWRKEVELLSDVAYFGLTTLAGYQTLG
EEYVSIIQVDPSRIHVPSSLRRGVLVTLHAVLPYLLDKALLPLEQELQADPDSGRPLQGSLGPGGRGCSG
ARRWMRHHTATLTEQQRRALLRAVFVLRQGLACLQRLHVAWFYIHGVFYHLAKRLTGITYLRVRSLPGED
LRARVSYRLLGVISLLHLVLSMGLQLYGFRQRQRARKEWRLHRGLSHRRASLEERAVSRNPLCTLCLEER
RHPTATPCGHLFCWECITAWCSSKAECPLCREKFPPQKLIYLRHYR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002617
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002617.4
RefSeq Size 2034 bp
RefSeq ORF 981 bp
Locus ID 5192
UniProt ID O60683, A0A024R068
Cytogenetics 1p36.32
Domains Pex2_Pex12, RING
Protein Families Druggable Genome, Transmembrane
MW 37.1 kDa
Summary This gene encodes a protein involved in import of peroxisomal matrix proteins. This protein localizes to the peroxisomal membrane. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome. Alternative splicing results in two transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002617" in other vectors (5)
SKU Description Size Price
RC202036L3 Lenti ORF clone of Human peroxisomal biogenesis factor 10 (PEX10), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC202036L4 Lenti ORF clone of Human peroxisomal biogenesis factor 10 (PEX10), transcript variant 2, mGFP tagged 10 ug
$600.00
RG202036 PEX10 (tGFP-tagged) - Human peroxisomal biogenesis factor 10 (PEX10), transcript variant 2 10 ug
$500.00
SC109564 PEX10 (untagged)-Human peroxisomal biogenesis factor 10 (PEX10), transcript variant 2 10 ug
$300.00
SC322418 PEX10 (untagged)-Human peroxisomal biogenesis factor 10 (PEX10), transcript variant 2 10 ug
$300.00

Plasmid Map for RC202036

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Cloning scheme for RC202036

Circular map for RC202036

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