GCNT3 (NM_004751) Human Tagged ORF Clone

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SKU
RC202007
GCNT3 (Myc-DDK-tagged)-Human glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GCNT3
Synonyms C2/4GnT; C2GNT2; C2GNTM; C24GNT; GNTM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202007 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCAATGGAAGAGACTCTGCCAGCTGCATTACTTGTGGGCTCTGGGCTGCTATATGCTGCTGGCCA
CTGTGGCTCTGAAACTTTCTTTCAGGTTGAAGTGTGACTCTGACCACTTGGGTCTGGAGTCCAGGGAATC
TCAAAGCCAGTACTGTAGGAATATCTTGTATAATTTCCTGAAACTTCCAGCAAAGAGGTCTATCAACTGT
TCAGGGGTCACCCGAGGGGACCAAGAGGCAGTGCTTCAGGCTATTCTGAATAACCTGGAGGTCAAGAAGA
AGCGAGAGCCTTTCACAGACACCCACTACCTCTCCCTCACCAGAGACTGTGAGCACTTCAAGGCTGAAAG
GAAGTTCATACAGTTCCCACTGAGCAAAGAAGAGGTGGAGTTCCCTATTGCATACTCTATGGTGATTCAT
GAGAAGATTGAAAACTTTGAAAGGCTACTGCGAGCTGTGTATGCCCCTCAGAACATATACTGTGTCCATG
TGGATGAGAAGTCCCCAGAAACTTTCAAAGAGGCGGTCAAAGCAATTATTTCTTGCTTCCCAAATGTCTT
CATAGCCAGTAAGCTGGTTCGGGTGGTTTATGCCTCCTGGTCCAGGGTGCAAGCTGACCTCAACTGCATG
GAAGACTTGCTCCAGAGCTCAGTGCCGTGGAAATACTTCCTGAATACATGTGGGACGGACTTTCCTATAA
AGAGCAATGCAGAGATGGTCCAGGCTCTCAAGATGTTGAATGGGAGGAATAGCATGGAGTCAGAGGTACC
TCCTAAGCACAAAGAAACCCGCTGGAAATATCACTTTGAGGTAGTGAGAGACACATTACACCTAACCAAC
AAGAAGAAGGATCCTCCCCCTTATAATTTAACTATGTTTACAGGGAATGCGTACATTGTGGCTTCCCGAG
ATTTCGTCCAACATGTTTTGAAGAACCCTAAATCCCAACAACTGATTGAATGGGTAAAAGACACTTATAG
CCCAGATGAACACCTCTGGGCCACCCTTCAGCGTGCACGGTGGATGCCTGGCTCTGTTCCCAACCACCCC
AAGTACGACATCTCAGACATGACTTCTATTGCCAGGCTGGTCAAGTGGCAGGGTCATGAGGGAGACATCG
ATAAGGGTGCTCCTTATGCTCCCTGCTCTGGAATCCACCAGCGGGCTATCTGCGTTTATGGGGCTGGGGA
CTTGAATTGGATGCTTCAAAACCATCACCTGTTGGCCAACAAGTTTGACCCAAAGGTAGATGATAATGCT
CTTCAGTGCTTAGAAGAATACCTACGTTATAAGGCCATCTATGGGACTGAACTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202007 protein sequence
Red=Cloning site Green=Tags(s)

MVQWKRLCQLHYLWALGCYMLLATVALKLSFRLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINC
SGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIH
EKIENFERLLRAVYAPQNIYCVHVDEKSPETFKEAVKAIISCFPNVFIASKLVRVVYASWSRVQADLNCM
EDLLQSSVPWKYFLNTCGTDFPIKSNAEMVQALKMLNGRNSMESEVPPKHKETRWKYHFEVVRDTLHLTN
KKKDPPPYNLTMFTGNAYIVASRDFVQHVLKNPKSQQLIEWVKDTYSPDEHLWATLQRARWMPGSVPNHP
KYDISDMTSIARLVKWQGHEGDIDKGAPYAPCSGIHQRAICVYGAGDLNWMLQNHHLLANKFDPKVDDNA
LQCLEEYLRYKAIYGTEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004751
ORF Size 1314 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004751.3
RefSeq Size 2240 bp
RefSeq ORF 1317 bp
Locus ID 9245
UniProt ID O95395, A0A024R5T9
Cytogenetics 15q22.2
Protein Families Transmembrane
Protein Pathways Metabolic pathways, O-Glycan biosynthesis
MW 50.9 kDa
Summary This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.provided by RefSeq, Apr 2009
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Documents
Resources
"NM_004751" in other vectors (6)
SKU Description Size Price
RC202007L1 Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3), Myc-DDK-tagged 10 ug
$986.00
RC202007L2 Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3), mGFP tagged 10 ug
$986.00
RC202007L3 Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3), Myc-DDK-tagged 10 ug
$986.00
RC202007L4 Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3), mGFP tagged 10 ug
$986.00
RG202007 GCNT3 (tGFP-tagged) - Human glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3) 10 ug
$489.00 $886.00
SC122687 GCNT3 (untagged)-Human glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3) 10 ug
$686.00

Plasmid Map for RC202007

Download sequence for

Cloning scheme for RC202007

Circular map for RC202007

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