NCKAP1 (NM_013436) Human Tagged ORF Clone
$982.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NCKAP1 |
| Synonyms | HEM2; NAP1; NAP125; p125Nap1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013436 |
| ORF Size | 3384 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013436.5 |
| RefSeq Size | 4997 bp |
| RefSeq ORF | 3387 bp |
| Locus ID | 10787 |
| UniProt ID | Q9Y2A7 |
| Cytogenetics | 2q32.1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 128.8 kDa |
| Summary | Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013436" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201960L1 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,282.00
|
|
| RC201960L2 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, mGFP tagged | 10 ug |
$1,282.00
|
|
| RC201960L3 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,282.00
|
|
| RC201960L4 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, mGFP tagged | 10 ug |
$1,282.00
|
|
| RG201960 | NCKAP1 (tGFP-tagged) - Human NCK-associated protein 1 (NCKAP1), transcript variant 1 | 10 ug |
$789.00
$1,182.00
|
|
| SC115236 | NCKAP1 (untagged)-Human NCK-associated protein 1 (NCKAP1), transcript variant 1 | 10 ug |
$983.00
|
|
Citations
Role of NCKAP1 in the Defective Phagocytic Function of Microglia-Like Cells Derived from Rapidly Progressing Sporadic ALS
Noh, Kwon, Oh et al
Mol Neurobiol (2023) 60 (8), 4761-4777 DOI: 10.1007/s12035-023-03339-2
Noh, Kwon, Oh et al
Mol Neurobiol (2023) 60 (8), 4761-4777 DOI: 10.1007/s12035-023-03339-2
Cited by 13
AI Summary: OriGene’s pLenti-C-mGFP-Human NCKAP1 (NM_013436) cDNA ORF Clone was used to overexpress NCKAP1 in microglia-like cells, enabling the researchers to demonstrate that restoring NCKAP1 levels could rescue defective phagocytosis associated with rapidly progressing sporadic ALS.
Snippet: man NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. iMGs were transduced with pLenti-C-mGFP-Human NCKAP1 (NM_013436), the cDNA ORF Clone (OriGene Technologies, Rockville, MD, USA), or pGFP-C-shLenti-NCKAP1 Human shRNA lentiviral particles (ID 10,787) according to the manufacturer’s protocol. … iMGs were transduced with pLenti-C-mGFP-Human NCKAP1 (NM_013436), the cDNA ORF Clone (OriGene Technologies, Rockville, MD, USA)
The WASF3-NCKAP1-CYFIP1 complex is essential for breast cancer metastasis
Teng, Qin, Bahssan et al
Cancer Res. (2016)
Teng, Qin, Bahssan et al
Cancer Res. (2016)
Showing 1-2 of 2 papers.
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