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RNF138 (NM_016271) Human Tagged ORF Clone

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SKU
RC201880
RNF138 (Myc-DDK-tagged)-Human ring finger protein 138 (RNF138), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF138
Synonyms hNARF; HSD-4; NARF; STRIN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201880 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGACCTCTCTGCGGCCACGTCCTACACCGAAGATGATTTCTACTGCCCCGTCTGTCAGGAGG
TGCTCAAAACGCCCGTGCGGACCACGGCCTGTCAGCACGTTTTCTGTAGAAAATGTTTCCTGACTGCAAT
GAGGGAAAGCGGAGCACATTGTCCCCTATGTCGTGGAAATGTGACTAGAAGAGAGAGAGCATGTCCTGAA
CGGGCCTTAGACCTTGAAAATATAATGAGGAAGTTTTCTGGTAGCTGCAGATGCTGTGCAAAACAGATTA
AATTCTATCGCATGAGACATCATTACAAATCTTGTAAGAAGTATCAGGATGAATATGGTGTTTCTTCTAT
CATTCCAAACTTTCAGATCTCTCAAGATTCAGTAGGGAACAGCAATAGGAGTGAAACATCCACATCTGAT
AACACAGAAACTTACCAAGAGAATACAAGTTCTTCTGGTCATCCTACTTTTAAGTGTCCCCTGTGTCAAG
AATCAAATTTTACCAGACAGCGTTTACTGGATCACTGTAACAGTAATCACCTATTTCAGATAGTTCCTGT
GACATGTCCTATTTGTGTGTCTCTTCCTTGGGGAGATCCTAGCCAGATTACCAGAAATTTCGTTAGTCAT
CTAAATCAGAGACATCAATTTGATTATGGAGAATTTGTGAATCTTCAGCTAGATGAAGAAACCCAATACC
AAACTGCTGTTGAAGAATCTTTTCAAGTAAACATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201880 protein sequence
Red=Cloning site Green=Tags(s)

MAEDLSAATSYTEDDFYCPVCQEVLKTPVRTTACQHVFCRKCFLTAMRESGAHCPLCRGNVTRRERACPE
RALDLENIMRKFSGSCRCCAKQIKFYRMRHHYKSCKKYQDEYGVSSIIPNFQISQDSVGNSNRSETSTSD
NTETYQENTSSSGHPTFKCPLCQESNFTRQRLLDHCNSNHLFQIVPVTCPICVSLPWGDPSQITRNFVSH
LNQRHQFDYGEFVNLQLDEETQYQTAVEESFQVNI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016271
ORF Size 735 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016271.5
RefSeq Size 3585 bp
RefSeq ORF 738 bp
Locus ID 51444
UniProt ID Q8WVD3, A0A140VJT9
Cytogenetics 18q12.1
Domains RING
MW 28.2 kDa
Summary The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. Alternatively spliced transcript variants encoding distinct isoforms have been observed. provided by RefSeq, Jul 2008
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Documents
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"NM_016271" in other vectors (7)
SKU Description Size Price
RC201880L1 Lenti ORF clone of Human ring finger protein 138 (RNF138), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC201880L2 Lenti ORF clone of Human ring finger protein 138 (RNF138), transcript variant 1, mGFP tagged 10 ug
$600.00
RC201880L3 Lenti ORF clone of Human ring finger protein 138 (RNF138), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC201880L4 Lenti ORF clone of Human ring finger protein 138 (RNF138), transcript variant 1, mGFP tagged 10 ug
$600.00
RG201880 RNF138 (tGFP-tagged) - Human ring finger protein 138 (RNF138), transcript variant 1 10 ug
$489.00 $500.00
SC114371 RNF138 (untagged)-Human ring finger protein 138 (RNF138), transcript variant 1 10 ug
$300.00
SC322447 RNF138 (untagged)-Human ring finger protein 138 (RNF138), transcript variant 1 10 ug
$300.00

Plasmid Map for RC201880

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201880
Circular map for RC201880

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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