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C19orf53 (NM_014047) Human Tagged ORF Clone

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SKU
RC201879
C19orf53 (Myc-DDK-tagged)-Human chromosome 19 open reading frame 53 (C19orf53)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C19orf53
Synonyms HSPC023; LYDG10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201879 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCAGGGGCAGCGCAAGTTTCAGGCGCACAAACCCGCAAAGAGTAAGACGGCAGCGGCAGCCTCTG
AAAAGAATCGGGGCCCAAGAAAAGGCGGTCGTGTTATCGCTCCCAAGAAGGCGCGCGTCGTGCAGCAGCA
AAAGCTCAAGAAGAACCTAGAAGTCGGAATCCGGAAGAAGATCGAACATGACGTGGTGATGAAAGCCAGC
AGCAGCCTGCCCAAGAAGCTGGCACTGCTGAAGGCCCCAGCCAAGAAGAAAGGGGCAGCTGCCGCCACCT
CCTCCAAGACACCTTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201879 protein sequence
Red=Cloning site Green=Tags(s)

MAQGQRKFQAHKPAKSKTAAAASEKNRGPRKGGRVIAPKKARVVQQQKLKKNLEVGIRKKIEHDVVMKAS
SSLPKKLALLKAPAKKKGAAAATSSKTPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014047
ORF Size 297 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014047.3
RefSeq Size 934 bp
RefSeq ORF 300 bp
Locus ID 28974
UniProt ID Q9UNZ5
Cytogenetics 19p13.13
MW 10.6 kDa
Summary May have a potential role in hypercalcemia of malignancy.UniProtKB/Swiss-Prot Function
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Documents
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"NM_014047" in other vectors (4)
SKU Description Size Price
RC201879L3 Lenti ORF clone of Human chromosome 19 open reading frame 53 (C19orf53), Myc-DDK-tagged 10 ug
$450.00
RC201879L4 Lenti ORF clone of Human chromosome 19 open reading frame 53 (C19orf53), mGFP tagged 10 ug
$450.00
RG201879 C19orf53 (tGFP-tagged) - Human chromosome 19 open reading frame 53 (C19orf53) 10 ug
$489.00
SC115192 C19orf53 (untagged)-Human chromosome 19 open reading frame 53 (C19orf53) 10 ug
$150.00

Plasmid Map for RC201879

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201879
Circular map for RC201879

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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