ARHGAP11A (NM_014783) Human Tagged ORF Clone
- TrueORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ARHGAP11A |
| Synonyms | GAP (1-12) |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
AscI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014783 |
| ORF Size | 3069 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014783.6 |
| RefSeq Size | 5616 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 9824 |
| UniProt ID | Q6P4F7 |
| Cytogenetics | 15q13.3 |
| Domains | RhoGAP |
| MW | 113.7 kDa |
| Summary | This gene encodes a member of the Rho GTPase activating protein family. In response to DNA damage, the encoded protein interacts with the p53 tumor suppressor protein and stimulates its tetramerization, which results in cell-cycle arrest and apoptosis. A chromosomal deletion that includes this gene is one cause of Prader-Willi syndrome, and an intronic variant of this gene may be associated with sleep duration in children. This gene is highly expressed in colon cancers and in a human basal-like breast cancer cell line. This gene also produces a ARHGAP11A-SCG5 readthrough transcript and ARHGAP11A-SCG5 protein. provided by RefSeq, Feb 2019 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014783" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201876L3 | Lenti-ORF clone of ARHGAP11A (Myc-DDK-tagged)-Human Rho GTPase activating protein 11A (ARHGAP11A), transcript variant 1 | 10 ug |
$1,725.00
|
|
| RC201876L4 | Lenti-ORF clone of ARHGAP11A (mGFP-tagged)-Human Rho GTPase activating protein 11A (ARHGAP11A), transcript variant 1 | 10 ug |
$1,725.00
|
|
| RG201876 | ARHGAP11A (tGFP-tagged) - Human Rho GTPase activating protein 11A (ARHGAP11A), transcript variant 1 | 10 ug |
$1,625.00
|
|
| SC310002 | ARHGAP11A (untagged)-Human Rho GTPase activating protein 11A (ARHGAP11A), transcript variant 1 | 10 ug |
$1,426.00
|
|
Citations
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