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WDR46 (NM_005452) Human Tagged ORF Clone

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SKU
RC201770
WDR46 (Myc-DDK-tagged)-Human WD repeat domain 46 (WDR46), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $568.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDR46
Synonyms BING4; C6orf11; FP221; UTP7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201770 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACAGCCCCCAAGCCGGGCAAGGATGTCCCGCCCAAGAAAGACAAACTTCAGACCAAGAGAAAGA
AACCGCGGCGATACTGGGAGGAAGAGACCGTTCCGACCACAGCCGGAGCCTCTCCAGGGCCTCCTCGTAA
CAAGAAGAATCGGGAGCTCCGTCCTCAGAGACCAAAAAATGCTTACATCTTAAAGAAGTCTCGGATCTCT
AAGAAGCCTCAGGTCCCGAAGAAACCCCGAGAATGGAAGAACCCGGAGTCCCAGCGCGGCTTGTCCGGGG
CCCAAGATCCATTCCCAGGCCCCGCCCCCGTCCCTGTGGAAGTGGTCCAGAAGTTCTGTCGCATTGACAA
ATCCCGAAAGCTACCACATTCTAAAGCCAAAACTCGAAGCCGACTTGAGGTGGCTGAAGCTGAGGAAGAG
GAAACAAGTATCAAAGCTGCTCGTTCTGAGCTGCTGCTTGCTGAAGAACCTGGGTTTCTGGAAGGGGAGG
ATGGGGAAGACACAGCAAAGATATGCCAGGCTGACATTGTGGAGGCTGTGGACATTGCAAGTGCAGCCAA
GCACTTTGACTTGAATCTGCGGCAGTTTGGACCCTACAGACTAAACTACTCTCGAACTGGAAGACACCTG
GCTTTTGGAGGGCGCCGAGGTCATGTGGCTGCCCTTGATTGGGTAACAAAGAAGCTTATGTGCGAGATCA
ACGTCATGGAGGCGGTGCGGGACATCCGGTTTCTCCATTCTGAGGCACTGCTTGCTGTTGCTCAGAACCG
CTGGCTCCACATCTATGACAATCAGGGCATTGAGCTCCACTGTATCCGCCGCTGTGACCGAGTAACACGG
CTTGAGTTCCTGCCCTTCCACTTCCTCCTGGCTACAGCTTCAGAAACAGGGTTTCTAACCTACCTGGATG
TGTCAGTGGGGAAGATTGTGGCAGCTCTGAATGCTCGAGCTGGGCGGCTCGATGTTATGAGTCAGAACCC
TTACAATGCCGTCATCCATCTCGGACACAGCAATGGTACTGTGTCTTTATGGAGTCCAGCTATGAAGGAG
CCACTGGCAAAGATTCTCTGTCATCGTGGTGGGGTCCGGGCTGTGGCAGTAGATTCTACAGGCACGTACA
TGGCCACCTCTGGCCTAGACCACCAGCTGAAGATCTTTGACTTGCGAGGGACGTACCAGCCTCTGAGCAC
TCGGACCCTGCCCCATGGAGCAGGGCACCTGGCCTTCTCCCAGAGGGGACTGCTGGTGGCGGGAATGGGT
GACGTTGTCAACATCTGGGCAGGGCAGGGCAAGGCCAGCCCACCCTCCCTTGAACAGCCCTACCTCACCC
ACCGGCTCTCAGGCCCTGTGCATGGCCTTCAGTTCTGCCCCTTTGAAGATGTGCTGGGGGTGGGGCACAC
TGGGGGCATCACCAGCATGCTGGTCCCTGGGGCCGGTGAGCCCAACTTCGATGGCCTGGAGAGTAATCCA
TACAGAAGCCGGAAGCAGCGCCAGGAGTGGGAGGTGAAGGCCCTGCTAGAGAAGGTACCTGCAGAGCTTA
TTTGTCTGGACCCACGAGCCCTGGCCGAGGTGGATGTCATCTCCCTGGAGCAGGGAAAGAAGGAGCAGAT
AGAGAGGCTGGGCTATGACCCGCAGGCTAAGGCTCCCTTCCAGCCAAAGCCAAAGCAGAAGGGCCGCAGC
TCCACGGCAAGCCTGGTGAAGAGGAAGAGGAAGGTCATGGATGAGGAACACAGGGACAAGGTCCGGCAGA
GCCTTCAGCAGCAGCATCATAAGGAGGCGAAGGCCAAGCCCACGGGGGCCCGGCCATCTGCCCTGGACAG
ATTTGTGCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201770 protein sequence
Red=Cloning site Green=Tags(s)

METAPKPGKDVPPKKDKLQTKRKKPRRYWEEETVPTTAGASPGPPRNKKNRELRPQRPKNAYILKKSRIS
KKPQVPKKPREWKNPESQRGLSGAQDPFPGPAPVPVEVVQKFCRIDKSRKLPHSKAKTRSRLEVAEAEEE
ETSIKAARSELLLAEEPGFLEGEDGEDTAKICQADIVEAVDIASAAKHFDLNLRQFGPYRLNYSRTGRHL
AFGGRRGHVAALDWVTKKLMCEINVMEAVRDIRFLHSEALLAVAQNRWLHIYDNQGIELHCIRRCDRVTR
LEFLPFHFLLATASETGFLTYLDVSVGKIVAALNARAGRLDVMSQNPYNAVIHLGHSNGTVSLWSPAMKE
PLAKILCHRGGVRAVAVDSTGTYMATSGLDHQLKIFDLRGTYQPLSTRTLPHGAGHLAFSQRGLLVAGMG
DVVNIWAGQGKASPPSLEQPYLTHRLSGPVHGLQFCPFEDVLGVGHTGGITSMLVPGAGEPNFDGLESNP
YRSRKQRQEWEVKALLEKVPAELICLDPRALAEVDVISLEQGKKEQIERLGYDPQAKAPFQPKPKQKGRS
STASLVKRKRKVMDEEHRDKVRQSLQQQHHKEAKAKPTGARPSALDRFVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005452
ORF Size 1830 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005452.4, NP_005443.2
RefSeq Size 2363 bp
RefSeq ORF 1833 bp
Locus ID 9277
UniProt ID O15213, A8K806
Cytogenetics 6p21.32
Domains WD40
Protein Families Druggable Genome
MW 68 kDa
Summary Scaffold component of the nucleolar structure. Required for localization of DDX21 and NCL to the granular compartment of the nucleolus.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_005452" in other vectors (4)
SKU Description Size Price
RC201770L3 Lenti ORF clone of Human WD repeat domain 46 (WDR46), transcript variant 1, Myc-DDK-tagged 10 ug
$868.00
RC201770L4 Lenti ORF clone of Human WD repeat domain 46 (WDR46), transcript variant 1, mGFP tagged 10 ug
$868.00
RG201770 WDR46 (tGFP-tagged) - Human WD repeat domain 46 (WDR46), transcript variant 1 10 ug
$489.00 $768.00
SC116743 WDR46 (untagged)-Human WD repeat domain 46 (WDR46), transcript variant 1 10 ug
$569.00

Plasmid Map for RC201770

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201770
Circular map for RC201770

file-alt Citations

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