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EIF2D (NM_006893) Human Tagged ORF Clone

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SKU
RC201726
EIF2D (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $544.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF2D
Synonyms HCA56; LGTN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201726 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGCCAAGGCCTTTCGGGTCAAGTCCAACACGGCCATCAAGGGGTCGGACAGGAGAAAGCTTCGAG
CTGATGTGACAACTGCTTTCCCCACCCTTGGAACTGATCAAGTCTCTGAGTTAGTACCTGGAAAGGAGGA
GCTCAACATTGTGAAGTTGTATGCTCACAAAGGGGATGCAGTGACTGTGTACGTGAGTGGTGGTAACCCC
ATCCTCTTTGAACTGGAGAAAAATCTGTATCCAACAGTGTACACGCTGTGGTCCTATCCTGATCTTCTGC
CAACCTTTACAACATGGCCTCTGGTGCTCGAGAAACTGGTAGGGGGAGCAGATTTGATGCTGCCTGGACT
GGTGATGCCCCCTGCTGGTCTGCCTCAGGTACAGAAGGGCGACCTCTGTGCCATTTCTTTGGTGGGGAAC
AGAGCCCCTGTAGCCATTGGAGTTGCAGCCATGTCCACAGCTGAGATGCTCACGTCAGGCCTGAAGGGAA
GGGGCTTCTCTGTGCTCCACACTTACCAGGACCACTTGTGGCGGTCTGGAAACAAGTCCTCTCCACCTTC
CATTGCTCCACTGGCCCTGGATTCAGCAGATCTCAGTGAAGAGAAGGGGTCTGTCCAGATGGACTCCACC
CTGCAGGGAGACATGAGGCACATGACCCTGGAGGGGGAAGAGGAGAATGGGGAGGTTCACCAGGCACGTG
AAGACAAGTCTCTCTCAGAAGCCCCAGAAGACACCAGCACCAGGGGCCTGAACCAAGACTCCACAGATAG
CAAAACGCTTCAAGAACAAATGGATGAGCTGTTACAGCAATGCTTCTTACATGCCTTGAAGTGCCGAGTC
AAAAAGGCTGACCTCCCTTTACTCACCAGCACTTTCCTTGGCAGCCACATGTTCTCCTGCTGCCCCGAAG
GACGACAACTGGACATAAAGAAGTCAAGCTACAAAAAGCTCTCTAAGTTCCTGCAGCAAATGCAGCAGGA
GCAGATTATACAGGTGAAGGAGCTGAGCAAAGGGGTGGAGAGCATTGTGGCTGTGGACTGGAAACACCCG
AGGATTACATCTTTCGTCATACCCGAGCCCTCCCCGACCTCCCAGACTATCCAGGAGGGTAGCAGGGAAC
AGCCCTATCACCCTCCAGATATAAAACCCCTCTACTGTGTCCCAGCCAGCATGACCCTGCTCTTCCAGGA
GTCTGGCCACAAGAAGGGGAGCTTTCTGGAGGGCAGTGAGGTCCGAACGATCGTCATTAACTACGCCAAG
AAAAATGACCTGGTTGATGCAGACAACAAAAATCTTGTGAGATTGGATCCCATCCTATGTGACTGCATCT
TAGAGAAAAATGAACAGCATACAGTCATGAAGCTTCCATGGGACAGTCTTCTGACCAGGTGTTTGGAAAA
ATTACAGCCTGCCTATCAAGTGACCCTTCCCGGACAAGAGCCCATTGTGAAGAAAGGGAGAATCTGTCCA
ATTGACATCACCCTAGCACAAAGAGCGTCTAATAAAAAGGTGACCGTGGTCCGGAACTTGGAGGCCTATG
GTCTGGACCCATACTCAGTGGCTGCCATCCTTCAGCAGCGATGCCAGGCTAGCACCACCGTCAATCCTGC
CCCTGGGGCCAAGGACAGCCTTCAGGTGCAGATCCAGGGAAACCAGGTCCACCACCTCGGCTGGCTATTG
CTTGAAGAGTATCAGCTCCCTCGAAAACACATCCAAGGTCTAGAAAAGGCCCTCAAACCTGGCAAGAAGA
AG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201726 protein sequence
Red=Cloning site Green=Tags(s)

MFAKAFRVKSNTAIKGSDRRKLRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDAVTVYVSGGNP
ILFELEKNLYPTVYTLWSYPDLLPTFTTWPLVLEKLVGGADLMLPGLVMPPAGLPQVQKGDLCAISLVGN
RAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDHLWRSGNKSSPPSIAPLALDSADLSEEKGSVQMDST
LQGDMRHMTLEGEEENGEVHQAREDKSLSEAPEDTSTRGLNQDSTDSKTLQEQMDELLQQCFLHALKCRV
KKADLPLLTSTFLGSHMFSCCPEGRQLDIKKSSYKKLSKFLQQMQQEQIIQVKELSKGVESIVAVDWKHP
RITSFVIPEPSPTSQTIQEGSREQPYHPPDIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAK
KNDLVDADNKNLVRLDPILCDCILEKNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGRICP
IDITLAQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQIQGNQVHHLGWLL
LEEYQLPRKHIQGLEKALKPGKKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006893
ORF Size 1752 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006893.3
RefSeq Size 2115 bp
RefSeq ORF 1755 bp
Locus ID 1939
UniProt ID P41214
Cytogenetics 1q32.1
Domains PUA, SUI1
Protein Families Druggable Genome
MW 64.7 kDa
Summary This gene encodes a translation initiation factor involved in the recruitment and delivery of aminoacyl-tRNAs to the P-site of the eukaryotic ribosome in a GTP-independent manner. This gene was previously referred to as ligatin, but is now known to localize to the cytoplasm and localize and function with translation factors. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2011
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Documents
Resources
"NM_006893" in other vectors (7)
SKU Description Size Price
RC201726L1 Lenti ORF clone of Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1, Myc-DDK-tagged 10 ug
$844.00
RC201726L2 Lenti ORF clone of Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1, mGFP tagged 10 ug
$844.00
RC201726L3 Lenti ORF clone of Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1, Myc-DDK-tagged 10 ug
$844.00
RC201726L4 Lenti ORF clone of Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1, mGFP tagged 10 ug
$844.00
RG201726 EIF2D (tGFP-tagged) - Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1 10 ug
$489.00 $744.00
SC115805 EIF2D (untagged)-Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1 10 ug
$545.00
SC322496 EIF2D (untagged)-Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1 10 ug
$545.00

Plasmid Map for RC201726

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201726
Circular map for RC201726

file-alt Citations

A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation
Sonobe, Aburas, Krishnan et al
Nat Commun (2021) 12 (1), 6025 DOI: 10.1038/s41467-021-26303-x
Cited by 49
AI Summary: OriGene's human eIF2D cDNA (RC201726) was used to construct the eIF2D-V5 Tag plasmid, enabling the study of eIF2D's role in RAN translation in this C. elegans ALS/FTD model.
Snippet: The eIF2D-V5 Tag plasmid was prepared by inserting human eIF2D cDNA (RC201726, Origene) into restriction sites of EcoRI and NotI of pcDNA6
FigureFigureFigure
Showing 1-1 of 1 papers.
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