ARPC5L (NM_030978) Human Tagged ORF Clone

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SKU
RC201699
ARPC5L (Myc-DDK-tagged)-Human actin related protein 2/3 complex, subunit 5-like (ARPC5L)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARPC5L
Synonyms ARC16-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201699 representing NM_030978
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGGAACACGCTGTCCTCGCGCTTCCGCCGGGTGGACATCGACGAATTTGACGAGAACAAATTTG
TGGACGAGCAGGAGGAGGCGGCGGCGGCGGCGGCGGAGCCAGGCCCGGACCCGAGCGAGGTGGACGGGCT
CCTGCGGCAAGGGGACATGCTTCGGGCATTCCATGCAGCCTTGCGGAACTCTCCCGTCAACACCAAGAAT
CAAGCTGTGAAGGAGCGAGCCCAGGGCGTGGTGCTGAAAGTGCTCACAAACTTCAAGAGCAGTGAGATTG
AGCAGGCTGTGCAGTCACTGGACAGAAACGGCGTTGACTTGTTAATGAAGTACATTTATAAAGGCTTTGA
GAAGCCCACAGAAAATAGCAGCGCAGTGTTACTCCAGTGGCACGAAAAGGCCTTAGCAGTAGGAGGACTA
GGCTCCATTATAAGAGTTCTTACAGCAAGAAAGACTGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201699 representing NM_030978
Red=Cloning site Green=Tags(s)

MARNTLSSRFRRVDIDEFDENKFVDEQEEAAAAAAEPGPDPSEVDGLLRQGDMLRAFHAALRNSPVNTKN
QAVKERAQGVVLKVLTNFKSSEIEQAVQSLDRNGVDLLMKYIYKGFEKPTENSSAVLLQWHEKALAVGGL
GSIIRVLTARKTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030978
ORF Size 459 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030978.3
RefSeq Size 1041 bp
RefSeq ORF 462 bp
Locus ID 81873
UniProt ID Q9BPX5, A0A024R897, B3KPC7
Cytogenetics 9q33.3
Domains p16_Arc
Protein Pathways Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
MW 16.8 kDa
Summary May function as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.UniProtKB/Swiss-Prot Function
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Documents
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"NM_030978" in other vectors (4)
SKU Description Size Price
RC201699L3 Lenti-ORF clone of ARPC5L (Myc-DDK-tagged)-Human actin related protein 2/3 complex, subunit 5-like (ARPC5L) 10 ug
$450.00
RC201699L4 Lenti-ORF clone of ARPC5L (mGFP-tagged)-Human actin related protein 2/3 complex, subunit 5-like (ARPC5L) 10 ug
$450.00
RG201699 ARPC5L (tGFP-tagged) - Human actin related protein 2/3 complex, subunit 5-like (ARPC5L) 10 ug
$350.00
SC109003 ARPC5L (untagged)-Human actin related protein 2/3 complex, subunit 5-like (ARPC5L) 10 ug
$150.00

Plasmid Map for RC201699

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Cloning scheme for RC201699

Circular map for RC201699

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