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THOC3 (NM_032361) Human Tagged ORF Clone

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SKU
RC201698
THOC3 (Myc-DDK-tagged)-Human THO complex 3 (THOC3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "THOC3 or NM_032361"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol THOC3
Synonyms hTREX45; THO3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201698 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTCCCCGCTGCAGCCATGGGGCCCTCGGCGTTGGGCCAGAGCGGCCCCGGCTCGATGGCCCCGT
GGTGCTCAGTGAGCAGCGGCCCGTCGCGCTACGTGCTTGGGATGCAGGAGCTGTTCCGGGGCCACAGCAA
GACGCGCGAGTTCCTGGCGCACAGCGCCAAGGTGCACTCGGTGGCCTGGAGTTGCGACGGGCGTCGCCTA
GCCTCGGGGTCCTTCGACAAGACGGCCAGCGTCTTCTTGCTGGAGAAGGACCGGTTGGTCAAAGAAAACA
ATTATCGGGGACATGGGGATAGTGTGGACCAGCTTTGTTGGCATCCAAGTAATCCTGACCTATTTGTTAC
GGCGTCTGGAGATAAAACCATTCGCATCTGGGATGTGAGGACTACAAAATGCATTGCCACTGTGAACACT
AAAGGGGAGAACATTAATATCTGCTGGAGTCCTGATGGGCAGACCATTGCTGTAGGCAACAAGGATGATG
TGGTGACCTTTATTGATGCCAAGACACACCGTTCCAAAGCAGAAGAGCAGTTCAAGTTCGAGGTCAACGA
AATCTCCTGGAACAATGACAATAATATGTTCTTCCTGACAAATGGCAATGGTTGTATCAACATCCTCAGC
TACCCAGAACTGAAGCCTGTGCAGTCCATCAACGCCCATCCTTCCAACTGCATCTGTATCAAGTTTGACC
CCATGGGGAAGTACTTTGCCACAGGAAGTGCAGATGCTTTGGTCAGCCTCTGGGATGTGGATGAGTTAGT
GTGTGTTCGGTGCTTTTCCAGGCTGGATTGGCCTGTAAGAACCCTCAGTTTCAGCCATGATGGGAAAATG
CTGGCGTCAGCATCGGAAGATCATTTTATTGACATTGCTGAAGTGGAGACAGGGGACAAACTATGGGAGG
TACAGTGTGAGTCTCCGACCTTCACAGTGGCGTGGCACCCCAAAAGGCCTCTGCTGGCATTTGCCTGTGA
TGACAAAGACGGCAAATATGACAGCAGCCGGGAAGCCGGAACTGTGAAGCTGTTTGGGCTTCCTAATGAT
TCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201698 protein sequence
Red=Cloning site Green=Tags(s)

MAVPAAAMGPSALGQSGPGSMAPWCSVSSGPSRYVLGMQELFRGHSKTREFLAHSAKVHSVAWSCDGRRL
ASGSFDKTASVFLLEKDRLVKENNYRGHGDSVDQLCWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNT
KGENINICWSPDGQTIAVGNKDDVVTFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILS
YPELKPVQSINAHPSNCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKM
LASASEDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLFGLPND
S

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032361
ORF Size 1053 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032361.1, NP_115737.1
RefSeq Size 1617 bp
RefSeq ORF 1056 bp
Locus ID 84321
UniProt ID Q96J01
Cytogenetics 5q35.2
Domains WD40
Protein Pathways Spliceosome
MW 38.8 kDa
Summary This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that the metazoan transcription export complex is involved in cell differentiation and development. A pseudogene of this gene has been defined on chromosome 5. provided by RefSeq, May 2013
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"NM_032361" in other vectors (7)
SKU Description Size Price
RC201698L1 Lenti ORF clone of Human THO complex 3 (THOC3), Myc-DDK-tagged 10 ug
$757.00
RC201698L2 Lenti ORF clone of Human THO complex 3 (THOC3), mGFP tagged 10 ug
$757.00
RC201698L3 Lenti ORF clone of Human THO complex 3 (THOC3), Myc-DDK-tagged 10 ug
$757.00
RC201698L4 Lenti ORF clone of Human THO complex 3 (THOC3), mGFP tagged 10 ug
$757.00
RG201698 THOC3 (tGFP-tagged) - Human THO complex 3 (THOC3) 10 ug
$489.00 $657.00
SC104947 THOC3 (untagged)-Human THO complex 3 (THOC3) 10 ug
$457.00
SC323789 THOC3 (untagged)-Human THO complex 3 (THOC3) 10 ug
$457.00

Plasmid Map for RC201698

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201698
Circular map for RC201698

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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