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SPATA5L1 (NM_024063) Human Tagged ORF Clone

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SKU
RC201675
SPATA5L1 (Myc-DDK-tagged)-Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $689.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPATA5L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201675 representing NM_024063
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCGGACTCGGATCCCTTCCCTGAAGGGCCGCTCTTAAAGCTGCTACCCTTAGACGCTAGAGACC
GGGGCACCCAGCGCTGCCGCCTGGGCCCGGCCGCCCTCCACGCCCTGGGCGCGCGCTTGGGCTCGGCAGT
GAAGATCTCGCTACCCGACGGCGGCTCCTGCCTCTGCACTGCCTGGCCTCGGCGGGACGGAGCGGACGGC
TTTGTGCAGCTGGACCCGCTGTGCGCGAGCCCCGGGGCGGCGGTCGGGGCGTCGAGATCCCGGAGGAGTC
TCAGCCTGAATCGCCTCCTCCTAGTGCCCTGTCCGCCCCTGCGGCGCGTCGCCGTGTGGCCGGTGTTGCG
AGAGCGGGCAGGCGCGCCCGGTGCCCGGAATACAGCCGCGGTGCTGGAGGCGGCACAGGAGCTGCTGAGA
AACCGACCGATCTCCCTGGGCCACGTGGTGGTCGCTCCGCCAGGCGCTCCTGGCCTGGTGGCTGCCTTGC
ACATCGTCGGCGGGACGCCCAGTCCCGATCCCGCTGGGCTGGTCACCCCTCGTACCCGCGTCAGCCTTGG
CGGGGAGCCTCCGTCGGAAGCCCAGCCGCAGCCCGAGGTGCCCCTGGGAGGTCTTTCGGAGGCGGCCGAC
TCGCTGCGGGAGCTCCTCCGCCTCCCGCTCCGCTACCCGCGCGCCCTGACCGCGCTGGGCTTAGCGGTGC
CTCGCGGGGTGCTCCTGGCGGGGCCCCCCGGAGTGGGCAAGACCCAGCTGGTGCAGGCCGTGGCGCGCGA
GGCGGGCGCGGAGCTGCTGGCAGTCAGCGCCCCGGCGCTGCAGGGTTCCCGGCCTGGGGAGACCGAGGAG
AACGTGCGGCGGGTCTTCCAGCGCGCCCGGGAACTGGCCAGCCGCGGACCCAGCCTCCTCTTCCTGGACG
AGATGGACGCCTTGTGTCCCCAGCGGGGCAGTCGAGCACCCGAGAGCCGCGTAGTGGCCCAGGTGTTGAC
GCTGCTGGACGGCGCCAGTGGGGACCGCGAGGTCGTGGTTGTGGGAGCCACTAACCGGCCGGACGCTCTA
GACCCAGCGCTGCGTAGACCCGGGAGATTTGACCGAGAGGTGGTCATTGGGACTCCCACACTTAAACAAA
GAAAGGAAATTCTGCAAGTGATTACCTCGAAGATGCCCATCTCCAGTCATGTTGATTTGGGCCTTCTTGC
AGAAATGACAGTTGGCTATGTTGGTGCCGACCTGACAGCACTCTGTAGGGAGGCTGCCATGCATGCCCTC
CTTCATAGTGAGAAGAACCAGGACAATCCTGTGATTGATGAAATAGACTTCCTTGAAGCTTTTAAAAATA
TTCAGCCCTCATCGTTTCGAAGCGTCATTGGATTAATGGATATCAAGCCTGTTGACTGGGAGGAGATTGG
TGGCCTTGAAGATGTAAAACTGAAGTTAAAACAGAGCATTGAGTGGCCTCTGAAATTCCCTTGGGAATTT
GTTAGAATGGGCCTGACACAACCAAAGGGAGTTCTCCTCTATGGGCCCCCTGGATGTGCTAAAACCACTC
TGGTGAGGGCCCTGGCCACAAGCTGTCACTGCTCTTTCGTTTCAGTGAGTGGAGCTGATCTGTTTTCACC
GTTTGTTGGAGATTCAGAAAAAGTGTTGTCTCAGATATTTCGACAAGCAAGAGCAAGCACTCCAGCAATT
TTGTTTTTGGATGAAATTGATTCAATCTTGGGAGCTCGCTCAGCCAGCAAGACAGGATGTGATGTTCAAG
AACGAGTTCTTTCTGTTCTCCTGAATGAATTAGATGGTGTTGGACTTAAGACAATAGAGAGAAGAGGAAG
TAAATCAAGTCAACAGGAGTTTCAAGAAGTTTTTAACCGAAGTGTCATGATTATTGCAGCAACAAATAGA
CCTGATGTGTTAGATACTGCTTTGTTACGACCTGGAAGATTAGATAAGATCATCTATATCCCACCTCCAG
ATCACAAGGGCAGGCTTTCTATTTTAAAAGTCTGTACAAAAACCATGCCAATAGGGCCTGATGTCTCCTT
AGAAAACCTCGCAGCAGAAACCTGTTTTTTTTCTGGAGCTGATCTTAGAAACCTCTGCACAGAAGCTGCT
TTGCTGGCTCTGCAAGAAAATGGACTAGACGCAACTACAGTGAAACAAGAGCACTTTCTAAAATCACTTA
AGACTGTAAAACCGTCGTTAAGTTGCAAGGACTTGGCTTTATATGAAAACTTATTTAAGAAAGAAGGATT
TTCTAACGTGGAAGGTATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201675 representing NM_024063
Red=Cloning site Green=Tags(s)

MAPDSDPFPEGPLLKLLPLDARDRGTQRCRLGPAALHALGARLGSAVKISLPDGGSCLCTAWPRRDGADG
FVQLDPLCASPGAAVGASRSRRSLSLNRLLLVPCPPLRRVAVWPVLRERAGAPGARNTAAVLEAAQELLR
NRPISLGHVVVAPPGAPGLVAALHIVGGTPSPDPAGLVTPRTRVSLGGEPPSEAQPQPEVPLGGLSEAAD
SLRELLRLPLRYPRALTALGLAVPRGVLLAGPPGVGKTQLVQAVAREAGAELLAVSAPALQGSRPGETEE
NVRRVFQRARELASRGPSLLFLDEMDALCPQRGSRAPESRVVAQVLTLLDGASGDREVVVVGATNRPDAL
DPALRRPGRFDREVVIGTPTLKQRKEILQVITSKMPISSHVDLGLLAEMTVGYVGADLTALCREAAMHAL
LHSEKNQDNPVIDEIDFLEAFKNIQPSSFRSVIGLMDIKPVDWEEIGGLEDVKLKLKQSIEWPLKFPWEF
VRMGLTQPKGVLLYGPPGCAKTTLVRALATSCHCSFVSVSGADLFSPFVGDSEKVLSQIFRQARASTPAI
LFLDEIDSILGARSASKTGCDVQERVLSVLLNELDGVGLKTIERRGSKSSQQEFQEVFNRSVMIIAATNR
PDVLDTALLRPGRLDKIIYIPPPDHKGRLSILKVCTKTMPIGPDVSLENLAAETCFFSGADLRNLCTEAA
LLALQENGLDATTVKQEHFLKSLKTVKPSLSCKDLALYENLFKKEGFSNVEGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024063
ORF Size 2259 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024063.1, NP_076968.1
RefSeq Size 2522 bp
RefSeq ORF 2262 bp
Locus ID 79029
UniProt ID Q9BVQ7
Cytogenetics 15q21.1
Domains AAA
MW 80.5 kDa
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Documents
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"NM_024063" in other vectors (7)
SKU Description Size Price
RC201675L1 Lenti ORF clone of Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1, Myc-DDK-tagged 10 ug
$989.00
RC201675L2 Lenti ORF clone of Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1, mGFP tagged 10 ug
$989.00
RC201675L3 Lenti ORF clone of Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1, Myc-DDK-tagged 10 ug
$989.00
RC201675L4 Lenti ORF clone of Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1, mGFP tagged 10 ug
$989.00
RG201675 SPATA5L1 (tGFP-tagged) - Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1 10 ug
$789.00 $889.00
SC111409 SPATA5L1 (untagged)-Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1 10 ug
$690.00
SC322179 SPATA5L1 (untagged)-Human spermatogenesis associated 5-like 1 (SPATA5L1), transcript variant 1 10 ug
$690.00

Plasmid Map for RC201675

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201675
Circular map for RC201675

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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