Beclin 1 (BECN1) (NM_003766) Human Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Beclin 1 |
| Synonyms | ATG6; beclin1; VPS30 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003766 |
| ORF Size | 1350 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003766.4 |
| RefSeq Size | 2144 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 8678 |
| UniProt ID | Q14457, A0A024R1X5 |
| Cytogenetics | 17q21.31 |
| Domains | APG6 |
| Protein Families | Druggable Genome |
| Protein Pathways | Regulation of autophagy |
| MW | 51.7 kDa |
| Summary | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003766" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201629L1 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC201629L2 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), mGFP tagged | 10 ug |
$986.00
|
|
| RC201629L3 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC201629L4 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), mGFP tagged | 10 ug |
$986.00
|
|
| RG201629 | BECN1 (tGFP-tagged) - Human beclin 1, autophagy related (BECN1) | 10 ug |
$886.00
|
|
| SC117750 | BECN1 (untagged)-Human beclin 1, autophagy related (BECN1) | 10 ug |
$686.00
|
|
| SC324265 | BECN1 (untagged)-Human beclin 1, autophagy related (BECN1) | 10 ug |
$686.00
|
|
Citations
IL-33 mediates Pseudomonas induced airway fibrogenesis and is associated with CLAD
Banday, Rao, Shankar et al
The Journal of Heart and Lung Transplantation (2022) 42 (1), 53-63 DOI: 10.1016/j.healun.2022.09.018
Banday, Rao, Shankar et al
The Journal of Heart and Lung Transplantation (2022) 42 (1), 53-63 DOI: 10.1016/j.healun.2022.09.018
Cited by 7
AI Summary: OriGene’s Beclin overexpression clone (CAT#: RC201629), containing human BECN1 in a pCMV6-Entry vector with a C-terminal MYCDDK tag, was used in this study to investigate the role of exogenous Beclin1 protein in airway fibrogenesis.
Snippet: The Beclin overexpression clone was procured from Origene (CAT#: RC201629). The said clone has human BECN1 cloned into pCMV6-Entry vector with a MYCDDK tag at its C terminal for the purpose of probing the exogenous protein. The overhang adds an
CaMKII-mediated Beclin 1 phosphorylation regulates autophagy that promotes degradation of Id and neuroblastoma cell differentiation
Li, Wu, Deng et al
Nat Commun (2017) 8 (1), 1159 DOI: 10.1038/s41467-017-01272-2
Li, Wu, Deng et al
Nat Commun (2017) 8 (1), 1159 DOI: 10.1038/s41467-017-01272-2
AI Summary: OriGene contributed to this study by providing the plasmid encoding human Beclin 1, which was used to investigate the role of Beclin 1 phosphorylation in autophagy and neuroblastoma cell differentiation.
Snippet: Article.;The plasmid encoding human Beclin 1 was purchased from OriGene (Rockville, MD, USA). The plasmid encoding human Id-1 and Id-2 were purchased from GenChem (Changzhou, China)
Showing 1-2 of 2 papers.
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