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Beclin 1 (BECN1) (NM_003766) Human Untagged Clone

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SKU
SC117750
BECN1 (untagged)-Human beclin 1, autophagy related (BECN1)
  $686.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Beclin 1
Synonyms ATG6; beclin1; VPS30
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117750 sequence for NM_003766 edited (data generated by NextGen Sequencing)
ATGGAAGGGTCTAAGACGTCCAACAACAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGC
TGCAGCCAGCCCCTGAAACTGGACACGAGTTTCAAGATCCTGGACCGTGTCACCATCCAG
GAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGGAGAGACCCAGGAGGAA
GAGACTAACTCAGGAGAGGAGCCATTTATTGAAACTCCTCGCCAGGATGGTGTCTCTCGC
AGATTCATCCCCCCAGCCAGGATGATGTCCACAGAAAGTGCCAACAGCTTCACTCTGATT
GGGGAGGCATCTGATGGCGGCACCATGGAGAACCTCAGCCGAAGACTGAAGGTCACTGGG
GACCTTTTTGACATCATGTCGGGCCAGACAGATGTGGATCACCCACTCTGTGAGGAATGC
ACAGATACTCTTTTAGACCAGCTGGACACTCAGCTCAACGTCACTGAAAATGAGTGTCAG
AACTACAAACGCTGTTTGGAGATCTTAGAGCAAATGAATGAGGATGACAGTGAACAGTTA
CAGATGGAGCTAAAGGAGCTGGCACTAGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGAC
GTGGAAAAGAACCGCAAGATAGTGGCAGAAAATCTCGAGAAGGTCCAGGCTGAGGCTGAG
AGACTGGATCAGGAGGAAGCTCAGTATCAGAGAGAATACAGTGAATTTAAACGACAGCAG
CTGGAGCTGGATGATGAGCTGAAGAGTGTTGAAAACCAGATGCGTTATGCCCAGACGCAG
CTGGATAAGCTGAAGAAAACCAACGTCTTTAATGCAACCTTCCACATCTGGCACAGTGGA
CAGTTTGGCACAATCAATAACTTCAGGCTGGGTCGCCTGCCCAGTGTTCCCGTGGAATGG
AATGAGATTAATGCTGCTTGGGGCCAGACTGTGTTGCTGCTCCATGCTCTGGCCAATAAG
ATGGGTCTGAAATTTCAGAGATACCGACTTGTTCCTTACGGAAACCATTCATATCTGGAG
TCTCTGACAGACAAATCTAAGGAGCTGCCGTTATACTGTTCTGGGGGGTTGCGGTTTTTC
TGGGACAACAAGTTTGACCATGCAATGGTGGCTTTCCTGGACTGTGTGCAGCAGTTCAAA
GAAGAGGTTGAGAAAGGCGAGACACGTTTTTGTCTTCCCTACAGGATGGATGTGGAGAAA
GGCAAGATTGAAGACACAGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAAC
TCTGAGGAGCAGTGGACAAAAGCTCTCAAGTTCATGCTGACGAATCTTAAGTGGGGTCTT
GCTTGGGTGTCCTCACAATTTTATAACAAATGA

Clone variation with respect to NM_003766.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003766 unedited
AAACAAACTTTACTATAGGCGGCCGCGCAATTCGCACGAGGCGGGAAGTCGTTGAAGACA
GAGCGATGGTAGTTCTGGAGGCCTCGCTCCGGGGCCGACCCGAGGCCACAGTGCCTCCGC
GGTAGACCGGACTTGGGTGACGGGCTCCGGGCTCCCGAGGTGAAGAGCATCGGGGGCTGA
GGGATGGAAGGGTCTAAGACGTCCAACAACAGCACCATGCAGGTGAGCTTCGTGTGCCAG
CGCTGCAGCCAGCCCCTGAAACTGGACACGAGTTTCAAGATCCTGGACCGTGTCACCATC
CAGGAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGGAGAGACCCAGGAG
GAAGAGACTAACTCAGGAGAGGAGCCATTTATTGAAACTCCTCGCCAGGATGGTGTCTCT
CGCAGATTCATCCCCCCAGCCAGGATGATGTCCACAGAAAGTGCCAACAGCTTCACTCTG
ATTGGGGAGGCATCTGATGGCGGCACCATGGAGAACCTCAGCCGAAGACTGAAGGTCACT
GGGGACCTTTTTGACATCATGTCGGGCCAGACAGATGTGGATCACCCACTCTGTGAGGAA
TGCACAGATACTCTTTTAGACCAGCTGGACACTCAGCTCAACGTCACTGAAAATGAGTGT
CAGAACTACAAACGCTGTTTGGAGATCTTAGAGCAAATGAATGAGGATGACAGTGAACAG
TTACAGATGGAGCTAAAGGAGCTGGCACTAGAGGAGGAGAGGCTGATCCAGGAGCTGGAA
GACGTGGAAAAAGAACCGCAGATAGTGGCAGAAAATCTCGAGAAGGTCCANNGCTGAGCT
GANAGACTGGATCANGNAGAAGCTCANTATCAGAGAGAATACAGTGAATTTAAACGACAG
CAGCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003766 unedited
CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAGTTTTCAG
ACTGCAGCAAATCTTTTATTACAAATAATTAAATCTCTCCATAATGTCTCAAACAGTATC
AAACACCATTTCATATCTCTAACACAGAGCAGAGTCGGCATTCAGTATAAGAACCAAGTG
AAAAGTGTTAAATTTCAAGCATCTGATCACATCACATGGTGACCAGGTAAAGCTTAGATG
TCATTTTCCCACATTATCCAACTGTGCATCTCAAACATATCCTCATCTCAGTAAAGACAA
AAGTTTCTATTTCATATTGTTAAGTGCAGGAAGTTGAGAGAGATAAAAATCCAGTGAAAA
CACATCAATCTCAATTCAACTCAGTTAAAAAAAAGAAAAGCAAATTTAAATTAGTTTTTT
TCAGAGAAGAAAGGGAAAGGAGTCCATGGGGTTAAGAATCAAAACTGACCAGGGCTGGCA
ACTATAGATGGCATGTTGTAGCTCTGGAAAGTATCTGTCACATGATATTTTAAAATAAAG
TGGCTTTTGTGGATTTTTTCTTTTTTGGTATTGTAAACATGTACTGTTTAATATTACCCG
AATTTAATTTAAAACATGTTTGCAAACAAAACAAAATTAAAAGCCTTTAAGGCAAACCCT
CCCCCTAAGGAAAAAAGTCATTTGTTATAAAATTGTGAGGACACCNCAAGCAGACCCCAC
TTAAGATTCGTCAGCATGAACTTGAGAGCTTTTGTCCACTGCTCCTCAGATTAAACTGGG
TTTTGATGGAATAGACCCGCACTGCCTNCTGTGTCTCAATCTGGCTTTCTNCCATCCATC
TGTAGGGAGACANAACGTGTCTCGCCTTCTCAACCTCTCTTTGACTGCTGCCCACAGTCA
GAAAGCACATGCAGG
Restriction Sites NotI-NotI |
ACCN NM_003766
Insert Size 2240 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003766.2, NP_003757.1
RefSeq Size 2144 bp
RefSeq ORF 1353 bp
Locus ID 8678
UniProt ID Q14457
Cytogenetics 17q21.31
Domains APG6
Protein Families Druggable Genome
Protein Pathways Regulation of autophagy
Summary This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 encode the same isoform.
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Documents
Resources
"NM_003766" in other vectors (7)
SKU Description Size Price
RC201629 BECN1 (Myc-DDK-tagged)-Human beclin 1, autophagy related (BECN1) 10 ug
$686.00
RC201629L1 Lenti ORF clone of Human beclin 1, autophagy related (BECN1), Myc-DDK-tagged 10 ug
$986.00
RC201629L2 Lenti ORF clone of Human beclin 1, autophagy related (BECN1), mGFP tagged 10 ug
$986.00
RC201629L3 Lenti ORF clone of Human beclin 1, autophagy related (BECN1), Myc-DDK-tagged 10 ug
$986.00
RC201629L4 Lenti ORF clone of Human beclin 1, autophagy related (BECN1), mGFP tagged 10 ug
$986.00
RG201629 BECN1 (tGFP-tagged) - Human beclin 1, autophagy related (BECN1) 10 ug
$886.00
SC324265 BECN1 (untagged)-Human beclin 1, autophagy related (BECN1) 10 ug
$686.00

file-alt Citations

Complex interplay between the length and composition of the huntingtin-derived peptides modulates the intracellular behavior of the N-terminal fragments of mutant huntingtin
Milewski, Gawliński, Bąk et al
Eur. J. Cell Biol. (2015)
Cited by 1
AI Summary: OriGene contributed to this study by providing the sequestosome (SQSTM1) cDNA (product SC117750), which was used to investigate the intracellular behavior of huntingtin fragments in transfected cells.
Snippet: Poland). The sequestosome (SQSTSM1) cDNA was purchased from OriGene (SC117750), subcloned into the pRK5-KS vector and modified (using site-directed mutagenesis) to introduce the sequence encoding epitope HA. ... Cite SaveSaving
Antagonism of tumoral prolactin receptor promotes autophagy-related cell death
Wen, Zand, Ozpolat et al
Cell Rep (2014) 7 (2), 488-500 DOI: 10.1016/j.celrep.2014.03.009
Cited by 53
AI Summary: In this study, OriGene products—including shRNA against BECN-1, shRNA against ATG7, siRNA against ATG5, and shRNA-PRLR—were used to selectively knock down key autophagy and prolactin receptor genes, enabling the authors to investigate the molecular mechanisms underlying autophagy-related cell death induced by PRL/PRLR antagonism in cancer cells.
Snippet: …The shRNA against BECN-1 or ATG7 and siRNA against ATG5 were purchased from OriGene. … Transfections of the pEGFP-tagged pCMV-prlr-ORF clone of PRLR, shRNA-PRLR, shATG7, or shRNA-BECN1 4 29-mer mix (human) were performed according to the manufacturer’s instructions. …
Inhibition of mTOR kinase by AZD8055 can antagonize chemotherapy-induced cell death through autophagy induction and down-regulation of p62/sequestosome 1
Huang, Yang, Yu et al
J. Biol. Chem. (2011) 286 (46), 40002-12 DOI: 10.1074/jbc.M111.297432
Cited by 10
AI Summary: OriGene’s p27 cDNA was used in this study to create a lentiviral expression construct, enabling investigation of the role of p27 in mTOR inhibition and autophagy-mediated cell survival during chemotherapy.
Snippet: Furthermore, autophagy-mediated down-regulation of p62/sequestosome 1 was shown to contribute to the pro-survival effect of autophagy. ... The p27 cDNA (Origene, Rockville, MD) was cloned into a lentiviral cDNA expression vector pCDH1-puro (System Biosciences, Mountain
Beclin 1 deficiency is associated with increased hypoxia-induced angiogenesis
Lee, Kim, Jin et al
Autophagy (2011) 7 (8), 829-39
Cited by 28
AI Summary: OriGene products contributed to this study by providing the pCMV vector containing mouse Beclin 1 cDNA (OriGene, SC117750) for Beclin 1 overexpression and the control pCMV vector (OriGene, pCMV6XL5) for transfection experiments in mouse lung endothelial cells.
Snippet: Autophagy Volume 7 Issue 8 Beclin 1 was overexpressed in mLECs by lipofectamine- mediated transfection of a pCMV vector (Origene, SC117750) containing cDNA encoding mouse Beclin 1. Lipofectamine transfection of pCMV vector (Origene, pCMV6XL5) served as control.
A natural BH3 mimetic induces autophagy in apoptosis-resistant prostate cancer via modulating Bcl-2-Beclin1 interaction at endoplasmic reticulum
Lian, Wu, He et al
Cell Death Differ (2011) 18 (1), 60-71 DOI: 10.1038/cdd.2010.74
Cited by 216
AI Summary: In this study, OriGene’s human Beclin1 shRNA plasmids and Atg5 shRNA plasmids were used to knock down Beclin1 and Atg5 expression, respectively, to demonstrate that (-)-gossypol-induced autophagy in prostate cancer cells is dependent on these genes.
Showing 1-5 of 5 papers.
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