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CD55 (NM_000574) Human Tagged ORF Clone

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SKU
RC201615
CD55 (Myc-DDK-tagged)-Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "CD55 or NM_000574"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD55
Synonyms CHAPLE; CR; CROM; DAF; TC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201615 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGTCGCGCGGCCGAGCGTGCCCGCGGCGCTGCCCCTCCTCGGGGAGCTGCCCCGGCTGCTGCTGC
TGGTGCTGTTGTGCCTGCCGGCCGTGTGGGGTGACTGTGGCCTTCCCCCAGATGTACCTAATGCCCAGCC
AGCTTTGGAAGGCCGTACAAGTTTTCCCGAGGATACTGTAATAACGTACAAATGTGAAGAAAGCTTTGTG
AAAATTCCTGGCGAGAAGGACTCAGTGATCTGCCTTAAGGGCAGTCAATGGTCAGATATTGAAGAGTTCT
GCAATCGTAGCTGCGAGGTGCCAACAAGGCTAAATTCTGCATCCCTCAAACAGCCTTATATCACTCAGAA
TTATTTTCCAGTCGGTACTGTTGTGGAATATGAGTGCCGTCCAGGTTACAGAAGAGAACCTTCTCTATCA
CCAAAACTAACTTGCCTTCAGAATTTAAAATGGTCCACAGCAGTCGAATTTTGTAAAAAGAAATCATGCC
CTAATCCGGGAGAAATACGAAATGGTCAGATTGATGTACCAGGTGGCATATTATTTGGTGCAACCATCTC
CTTCTCATGTAACACAGGGTACAAATTATTTGGCTCGACTTCTAGTTTTTGTCTTATTTCAGGCAGCTCT
GTCCAGTGGAGTGACCCGTTGCCAGAGTGCAGAGAAATTTATTGTCCAGCACCACCACAAATTGACAATG
GAATAATTCAAGGGGAACGTGACCATTATGGATATAGACAGTCTGTAACGTATGCATGTAATAAAGGATT
CACCATGATTGGAGAGCACTCTATTTATTGTACTGTGAATAATGATGAAGGAGAGTGGAGTGGCCCACCA
CCTGAATGCAGAGGAAAATCTCTAACTTCCAAGGTCCCACCAACAGTTCAGAAACCTACCACAGTAAATG
TTCCAACTACAGAAGTCTCACCAACTTCTCAGAAAACCACCACAAAAACCACCACACCAAATGCTCAAGC
AACACGGAGTACACCTGTTTCCAGGACAACCAAGCATTTTCATGAAACAACCCCAAATAAAGGAAGTGGA
ACCACTTCAGGTACTACCCGTCTTCTATCTGGGCACACGTGTTTCACGTTGACAGGTTTGCTTGGGACGC
TAGTAACCATGGGCTTGCTGACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201615 protein sequence
Red=Cloning site Green=Tags(s)

MTVARPSVPAALPLLGELPRLLLLVLLCLPAVWGDCGLPPDVPNAQPALEGRTSFPEDTVITYKCEESFV
KIPGEKDSVICLKGSQWSDIEEFCNRSCEVPTRLNSASLKQPYITQNYFPVGTVVEYECRPGYRREPSLS
PKLTCLQNLKWSTAVEFCKKKSCPNPGEIRNGQIDVPGGILFGATISFSCNTGYKLFGSTSSFCLISGSS
VQWSDPLPECREIYCPAPPQIDNGIIQGERDHYGYRQSVTYACNKGFTMIGEHSIYCTVNNDEGEWSGPP
PECRGKSLTSKVPPTVQKPTTVNVPTTEVSPTSQKTTTKTTTPNAQATRSTPVSRTTKHFHETTPNKGSG
TTSGTTRLLSGHTCFTLTGLLGTLVTMGLLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000574
ORF Size 1143 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000574.5
RefSeq Size 2796 bp
RefSeq ORF 1146 bp
Locus ID 1604
UniProt ID P08174
Cytogenetics 1q32.2
Domains CCP
Protein Families Druggable Genome
Protein Pathways Complement and coagulation cascades, Hematopoietic cell lineage, Viral myocarditis
MW 41.4 kDa
Summary This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. provided by RefSeq, Jul 2014
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Documents
Resources
"NM_000574" in other vectors (7)
SKU Description Size Price
RC201615L1 Lenti ORF clone of Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201615L2 Lenti ORF clone of Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1, mGFP tagged 10 ug
$757.00
RC201615L3 Lenti ORF clone of Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201615L4 Lenti ORF clone of Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1, mGFP tagged 10 ug
$757.00
RG201615 CD55 (tGFP-tagged) - Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1 10 ug
$489.00 $657.00
SC119802 CD55 (untagged)-Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1 10 ug
$457.00
SC322226 CD55 (untagged)-Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1 10 ug
$457.00

Plasmid Map for RC201615

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201615
Circular map for RC201615

file-alt Citations

Epidermal Growth Factor Receptor Targeting IgG3 Triggers Complement-Mediated Lysis of Decay-Accelerating Factor Expressing Tumor Cells through the Alternative Pathway Amplification Loop
Rösner, Lohse, Peipp et al
J. Immunol. (2014)
Cited by 19
AI Summary: OriGene products, specifically the PS100001 vector and the pCMV6-Entry-CD55 vector (catalog no. RC201615), were used for overexpression studies to investigate the role of CD55 in complement-mediated lysis of tumor cells.
Snippet: PS100001;OriGene Technologies, Rockville, MD) or pCMV6-Entry- CD55 vector (catalog no. RC201615;OriGene Technologies) by lip- ofection using Lipofectamine 2000 (Invitrogen) according to the manu- facturer's instructions. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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