MLH1 (NM_000249) Human Tagged ORF Clone
SKU
RC201607
MLH1 (Myc-DDK-tagged)-Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1
$692.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MLH1 |
| Synonyms | COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000249 |
| ORF Size | 2268 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000249.4 |
| RefSeq Size | 2662 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 4292 |
| UniProt ID | P40692 |
| Cytogenetics | 3p22.2 |
| Domains | DNA_mis_repair, HATPase_c |
| Protein Families | Druggable Genome |
| Protein Pathways | Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer |
| MW | 84.6 kDa |
| Summary | The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000249" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201607L1 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$992.00
|
|
| RC201607L2 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, mGFP tagged | 10 ug |
$992.00
|
|
| RC201607L3 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$992.00
|
|
| RC201607L4 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, mGFP tagged | 10 ug |
$992.00
|
|
| RG201607 | MLH1 (tGFP-tagged) - Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1 | 10 ug |
$789.00
$892.00
|
|
| SC119993 | MLH1 (untagged)-Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1 | 10 ug |
$693.00
|
|
| SC323792 | MLH1 (untagged)-Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1 | 10 ug |
$693.00
|
|
Citations
Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair
Colicino-Murbach, Hathaway, Dungrawala
Nucleic Acids Res (2024)
Colicino-Murbach, Hathaway, Dungrawala
Nucleic Acids Res (2024)
Cited by 8
AI Summary: OriGene’s CMV-MSH2 Myc-DDK-tagged (RC205848) and CMV-MLH1 Myc-DDK-tagged (RC201607) plasmids were used for overexpression studies to investigate the roles of DNA mismatch repair proteins in nascent strand degradation at stalled replication forks.
Snippet: mid transfections, RPE-1 cells were transfected using Lipofectamine 2000 (Invitrogen 11668027) in Opti-MEM Reduced Serum Media for 48–72 h using the following constructs: CMV-hRAD51 (Addgene Plasmid #125570), CMVhRAD51K133R (Addgene Plasmid #125571), CMV-MSH2 Myc-DDK-tagged (Origene RC205848) and CMV-MLH1 Myc-DDK-tagged (Origene RC201607)
Dianhydrogalactitol synergizes with topoisomerase poisons to overcome DNA repair activity in tumor cells
Zhai, Li, Kotapalli et al
Cell Death Dis (2020) 11 (7), 577 DOI: 10.1038/s41419-020-02780-8
Zhai, Li, Kotapalli et al
Cell Death Dis (2020) 11 (7), 577 DOI: 10.1038/s41419-020-02780-8
Cited by 8
AI Summary: OriGene products contributed to this study by providing human MLH1 cDNA (vector RC201607), MSH2 cDNA (vector RC205848), and the pLenti-C-Myc-DDK-P2A-Puro vector (PS100092), which were used to generate stable cell lines for investigating the role of DNA repair pathways in tumor cell response to dianhydrogalactitol and chemotherapy.
Snippet: B3, siBRCA1–25 (SI04381377, Qiagen);B4, siBRCA1–15 (SI02664368, Qiagen);and B5, siBRCA1–17 (SI03103975, Qiagen). STABLE CELL LINE GENERATION Human MLH1 cDNA (obtained from vector RC201607, OriGene Technologies, Rockville, MD, USA) or MSH2 cDNA (obtained from vector RC205848, OriGene Technologies) was subcloned into pLenti-C-Myc-DDK-P2A-Puro vector (PS100092, OriGene Technologies) using;- 1,2:5,6-Dianhydrogalactitol (DAG) is a bi-functional DNA-targeting agent currently in phase II clinical trial for treatment of temozolomide-resistant glioblastoma (GBM). In the present study, we investigated the cytotoxic activity of DAG alone or in combination with common;and B5, siBRCA1–17 (SI03103975, Qiagen). Stable cell line generation Human MLH1 cDNA (obtained from vector RC201607, OriGene Technologies, Rockville, MD, USA) or MSH2 cDNA (obtained from vector RC205848, OriGene Technologies) was subcloned into pLenti-C-Myc-DDK-P2A-Puro vector (PS100092, OriGene Technologies) using
Retinal mitochondrial DNA mismatch repair in the development of diabetic retinopathy, and its continued progression after termination of hyperglycemia
Mishra, Kowluru
Invest. Ophthalmol. Vis. Sci. (2014)
Mishra, Kowluru
Invest. Ophthalmol. Vis. Sci. (2014)
Cited by 74
Showing 1-3 of 3 papers.
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