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JTB (NM_006694) Human Tagged ORF Clone

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SKU
RC201578
JTB (Myc-DDK-tagged)-Human jumping translocation breakpoint (JTB)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "JTB or NM_006694"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol JTB
Synonyms hJT; HJTB; HSPC222; PAR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201578 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGCGGGTGCCGGGAGGCCTGGCCTCCCCCAGGGCCGCCACCTCTGCTGGTTGCTCTGTGCTTTCA
CCTTAAAGCTCTGCCAAGCAGAGGCTCCCGTGCAGGAAGAGAAGCTGTCAGCAAGCACCTCAAATTTGCC
ATGCTGGCTGGTGGAAGAGTTTGTGGTAGCAGAAGAGTGCTCTCCATGCTCTAATTTCCGGGCTAAAACT
ACCCCTGAGTGTGGTCCCACAGGATATGTAGAGAAAATCACATGCAGCTCATCTAAGAGAAATGAGTTCA
AAAGCTGCCGCTCAGCTTTGATGGAACAACGCTTATTTTGGAAGTTCGAAGGGGCTGTCGTGTGTGTGGC
CCTGATCTTCGCTTGTCTTGTCATCATTCGTCAGCGACAATTGGACAGAAAGGCTCTGGAAAAGGTCCGG
AAGCAAATCGAGTCCATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201578 protein sequence
Red=Cloning site Green=Tags(s)

MLAGAGRPGLPQGRHLCWLLCAFTLKLCQAEAPVQEEKLSASTSNLPCWLVEEFVVAEECSPCSNFRAKT
TPECGPTGYVEKITCSSSKRNEFKSCRSALMEQRLFWKFEGAVVCVALIFACLVIIRQRQLDRKALEKVR
KQIESI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006694
ORF Size 438 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006694.4
RefSeq Size 1574 bp
RefSeq ORF 441 bp
Locus ID 10899
UniProt ID O76095
Cytogenetics 1q21.3
Protein Families Transmembrane
MW 16.4 kDa
Summary Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1 (By similarity). Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential (By similarity).UniProtKB/Swiss-Prot Function
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"NM_006694" in other vectors (5)
SKU Description Size Price
RC201578L3 Lenti ORF clone of Human jumping translocation breakpoint (JTB), Myc-DDK-tagged 10 ug
$450.00
RC201578L4 Lenti ORF clone of Human jumping translocation breakpoint (JTB), mGFP tagged 10 ug
$450.00
RG201578 JTB (tGFP-tagged) - Human jumping translocation breakpoint (JTB) 10 ug
$489.00
SC115956 JTB (untagged)-Human jumping translocation breakpoint (JTB) 10 ug
$150.00
SC323778 JTB (untagged)-Human jumping translocation breakpoint (JTB) 10 ug
$150.00

Plasmid Map for RC201578

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201578
Circular map for RC201578

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