JTB (NM_006694) Human Tagged Lenti ORF Clone
- LentiORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | JTB |
| Synonyms | hJT; HJTB; HSPC222; PAR |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006694 |
| ORF Size | 438 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006694.2 |
| RefSeq Size | 1574 bp |
| RefSeq ORF | 441 bp |
| Locus ID | 10899 |
| UniProt ID | O76095 |
| Cytogenetics | 1q21.3 |
| Protein Families | Transmembrane |
| MW | 16.4 kDa |
| Summary | Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1 (By similarity). Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006694" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201578 | JTB (Myc-DDK-tagged)-Human jumping translocation breakpoint (JTB) | 10 ug |
$150.00
|
|
| RC201578L3 | Lenti ORF clone of Human jumping translocation breakpoint (JTB), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RG201578 | JTB (tGFP-tagged) - Human jumping translocation breakpoint (JTB) | 10 ug |
$489.00
|
|
| SC115956 | JTB (untagged)-Human jumping translocation breakpoint (JTB) | 10 ug |
$150.00
|
|
| SC323778 | JTB (untagged)-Human jumping translocation breakpoint (JTB) | 10 ug |
$150.00
|
|
Citations
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