TTC4 (NM_004623) Human Tagged ORF Clone

  • TrueORF®
SKU
RC201570
TTC4 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 4 (TTC4)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TTC4
Synonyms CNS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201570 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACAACCTGGGCAGGATCCCACCTCAGACGACGTCATGGACTCGTTCCTGGAAAAGTTCCAGAGCC
AGCCTTACCGTGGCGGCTTTCATGAGGACCAGTGGGAGAAGGAATTTGAAAAGGTCCCCCTATTTATGAC
GAGAGCGCCATCAGAAATTGATCCCAGGGAGAATCCTGACTTGGCTTGTCTCCAGTCAATTATTTTTGAT
GAGGAGCGTTCTCCAGAAGAACAGGCCAAGACCTATAAAGATGAGGGCAATGATTACTTTAAAGAAAAAG
ACTACAAGAAAGCTGTAATTTCATACACTGAAGGCTTAAAGAAGAAATGTGCAGATCCTGATTTGAATGC
TGTCCTTTATACCAACCGGGCAGCAGCACAGTACTATCTGGGCAATTTTCGTTCTGCTCTCAATGATGTG
ACAGCTGCCAGAAAGCTAAAACCCTGCCACCTCAAAGCAATAATAAGAGGTGCCTTATGCCATCTGGAAC
TGAAACACTTTGCCGAGGCCGTGAACTGGTGTGATGAGGGACTGCAAATAGATGCCAAAGAGAAGAAGCT
TCTGGAAATGAGGGCTAAAGCAGACAAGCTGAAGCGAATTGAACAGAGGGATGTGAGGAAAGCCAACTTG
AAAGAAAAGAAGGAGAGGAATCAGAATGAGGCTTTACTCCAGGCCATCAAGGCTAGGAATATCAGGCTCT
CAGAAGCTGCCTGTGAGGATGAAGATTCAGCCTCAGAAGGTCTAGGTGAGCTTTTCCTGGATGGACTCAG
CACTGAGAACCCCCATGGAGCCAGGCTGAGTCTAGATGGCCAGGGCAGGCTGAGCTGGCCTGTGCTCTTT
CTGTACCCAGAGTATGCCCAGTCGGACTTCATCTCTGCTTTTCATGAGGACTCCAGGTTTATTGATCATC
TAATGGTGATGTTTGGTGAAACACCCTCTTGGGACCTAGAGCAAAAATATTGCCCTGATAATTTGGAGGT
CTACTTTGAGGATGAGGACAGGGCAGAACTATACCGGGTGCCTGCCAAGAGCACCTTGCTACAGGTTCTA
CAGCACCAGAGGTACTTTGTAAAAGCCCTGACACCAGCATTTTTGGTCTGTGTAGGATCCTCTCCTTTTT
GCAAGAATTTTCTCCGGGGGAGAAAGGTGTACCAGATACGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201570 protein sequence
Red=Cloning site Green=Tags(s)

MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMTRAPSEIDPRENPDLACLQSIIFD
EERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDV
TAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANL
KEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLF
LYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVL
QHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004623
ORF Size 1161 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004623.2, NP_004614.2
RefSeq Size 2395 bp
RefSeq ORF 1164 bp
Locus ID 7268
UniProt ID O95801
Cytogenetics 1p32.3
Domains TPR
MW 44.7 kDa
Summary This gene encodes a protein that contains tetratricopeptide (TPR) repeats, which often mediate protein-protein interactions and chaperone activity. The encoded protein interacts with heat shock proteins 70 and 90. Alternative splicing results in multiple transcript variants. Naturally-occuring readthrough transcription occurs from upstream gene MROH (maestro heat-like repeat family member 7) to this gene. provided by RefSeq, Apr 2014
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Documents
Resources
"NM_004623" in other vectors (5)
SKU Description Size Price
RC201570L3 Lenti-ORF clone of TTC4 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 4 (TTC4) 10 ug
$757.00
RC201570L4 Lenti-ORF clone of TTC4 (mGFP-tagged)-Human tetratricopeptide repeat domain 4 (TTC4) 10 ug
$757.00
RG201570 TTC4 (tGFP-tagged) - Human tetratricopeptide repeat domain 4 (TTC4) 10 ug
$489.00 $657.00
SC117229 TTC4 (untagged)-Human tetratricopeptide repeat domain 4 (TTC4) 10 ug
$457.00
SC323797 TTC4 (untagged)-Human tetratricopeptide repeat domain 4 (TTC4) 10 ug
$457.00

Plasmid Map for RC201570

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Cloning scheme for RC201570

Circular map for RC201570

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