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TRMT6 (NM_015939) Human Tagged ORF Clone

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SKU
RC201534
TRMT6 (Myc-DDK-tagged)-Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRMT6
Synonyms CGI-09; GCD10; Gcd10p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201534 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGCTCAGGGGAGCAGCCGGGCCCACAACCACAGCATCCCGGAGACCACCGCATCCGCGACGGCG
ACTTCGTGGTGCTGAAACGTGAAGATGTGTTTAAAGCAGTACAAGTCCAGCGGAGAAAAAAAGTAACTTT
CGAAAAACAGTGGTTCTACCTGGATAACGTCATTGGCCATAGTTATGGAACTGCATTTGAAGTGACCAGT
GGAGGAAGTCTACAGCCCAAGAAGAAGAGGGAAGAGCCTACTGCAGAGACTAAAGAAGCGGGCACTGATA
ATCGAAATATAGTTGATGATGGGAAATCTCAGAAACTTACTCAAGATGACATAAAAGCTTTGAAGGACAA
GGGCATTAAAGGAGAGGAAATAGTTCAGCAGTTAATTGAAAATAGTACAACATTCCGAGACAAGACAGAA
TTTGCCCAAGATAAATATATTAAAAAGAAGAAAAAAAAATATGAAGCCATCATTACTGTTGTGAAGCCAT
CCACCCGTATTCTTTCAATTATGTATTATGCAAGAGAACCTGGAAAAATTAACCACATGAGATACGATAC
ACTAGCCCAGATGTTGACGTTGGGAAATATCCGTGCTGGCAACAAAATGATTGTGATGGAAACGTGTGCA
GGCTTGGTGCTGGGTGCAATGATGGAACGAATGGGAGGTTTTGGCTCCATTATTCAGCTATACCCTGGAG
GAGGACCTGTTCGGGCAGCAACAGCATGTTTTGGATTTCCCAAATCTTTTCTCAGTGGTCTTTATGAATT
CCCTCTCAACAAAGTGGACAGTCTTCTACATGGAACATTTTCTGCCAAGATGTTATCTTCAGAGCCAAAA
GACAGTGCTTTGGTTGAAGAAAGTAATGGCACACTGGAGGAAAAACAGGCTTCTGAACAAGAGAATGAAG
ACAGCATGGCAGAGGCCCCAGAGAGCAACCACCCAGAAGACCAGGAAACAATGGAAACAATTTCTCAAGA
TCCAGAACATAAGGGGCCTAAAGAGAGAGGAAGCAAAAAAGATTATATTCAGGAAAAACAGAGGAGACAA
GAAGAGCAGAGGAAAAGACATTTAGAGGCTGCCGCTCTGCTGAGTGAAAGAAACGCAGATGGTTTAATTG
TAGCTAGTCGTTTCCACCCCACTCCCCTGCTGCTGTCTTTGCTGGACTTTGTGGCCCCTTCAAGGCCGTT
TGTGGTCTACTGTCAGTACAAAGAGCCTCTGTTGGAATGCTACACAAAACTGCGGGAGAGGGGAGGGGTC
ATCAACCTCAGGCTGTCTGAAACCTGGCTCAGAAATTATCAGGTTTTGCCAGATCGAAGTCATCCTAAAC
TGCTGATGAGTGGAGGTGGGGGTTATCTTCTCTCCGGCTTCACCGTTGCCATGGACAACCTTAAAGCAGA
CACCAGCCTCAAATCTAATGCAAGCACTTTAGAATCACACGAGACTGAGGAGCCTGCAGCTAAAAAACGA
AAATGCCCAGAGTCTGACTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201534 protein sequence
Red=Cloning site Green=Tags(s)

MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTS
GGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTE
FAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCA
GLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPK
DSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQ
EEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGV
INLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKR
KCPESDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015939
ORF Size 1491 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015939.5
RefSeq Size 2353 bp
RefSeq ORF 1494 bp
Locus ID 51605
UniProt ID Q9UJA5
Cytogenetics 20p12.3
MW 55.8 kDa
Summary This gene encodes a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2013
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Documents
Resources
"NM_015939" in other vectors (4)
SKU Description Size Price
RC201534L3 Lenti ORF clone of Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6), Myc-DDK-tagged 10 ug
$757.00
RC201534L4 Lenti ORF clone of Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6), mGFP tagged 10 ug
$757.00
RG201534 TRMT6 (tGFP-tagged) - Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6) 10 ug
$657.00
SC126188 TRMT6 (untagged)-Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6) 10 ug
$457.00

Plasmid Map for RC201534

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201534
Circular map for RC201534

file-alt Citations

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution
Safra, Sas-Chen, Nir et al
Nature (2017) 551 (7679), 251-255 DOI: 10.1038/nature24456
Cited by 676
AI Summary: OriGene products contributed to this study by providing plasmids encoding full-length TRMT6 and TRMT61A, which were used for overexpression experiments to investigate the enzymatic machinery responsible for m1A modification on mRNA.
Snippet: As negative controls, we used Ambion In Vivo Negative Control #2 siRNA (catalogue number 4390846). Cells were harvested at 96 h. For overexpression, plasmids encoding full-length TRMT6/ TRMT61A under a cytomegalovirus promoter were obtained from OriGene
Showing 1-1 of 1 papers.
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