MOSPD1 (NM_019556) Human Tagged ORF Clone

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SKU
RC201517
MOSPD1 (Myc-DDK-tagged)-Human motile sperm domain containing 1 (MOSPD1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MOSPD1
Synonyms DJ473B4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201517 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCAACAAAAAAGACAACCAGAGTTAGTGGAAGGAAATCTTCCTGTTTTCGTGTTCCCCACGGAGC
TCATATTTTATGCAGATGATCAGTCAACACATAAGCAAGTGTTGACACTGTACAATCCCTATGAGTTTGC
CTTAAAGTTCAAAGTTTTGTGTACTACTCCAAATAAGTATGTTGTCGTTGATGCTGCAGGTGCAGTAAAG
CCTCAGTGTTGTGTGGATATTGTGATTCGTCATCGAGATGTTCGATCCTGTCACTATGGTGTAATAGACA
AATTCCGTCTCCAAGTTTCCGAGCAAAGCCAAAGGAAGGCTTTGGGAAGAAAAGAGGTTGTTGCTACTCT
TCTCCCATCAGCAAAAGAACAACAAAAGGAAGAAGAGGAAAAAAGATTAAAGGAACATTTAACTGAAAGT
TTATTTTTTGAGCAGTCGTTTCAACCAGAAAACAGAGCTGTATCCTCAGGACCTAGTTTACTAACTGTCT
TCCTGGGAGTGGTGTGCATTGCAGCCCTGATGCTGCCTACACTGGGGGATGTGGAATCGCTGGTGCCTCT
CTACCTCCACTTAAGTGTGAATCAAAAATTAGTGGCTGCTTATATCTTAGGTCTTATCACAATGGCCATA
CTTAGAACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201517 protein sequence
Red=Cloning site Green=Tags(s)

MHQQKRQPELVEGNLPVFVFPTELIFYADDQSTHKQVLTLYNPYEFALKFKVLCTTPNKYVVVDAAGAVK
PQCCVDIVIRHRDVRSCHYGVIDKFRLQVSEQSQRKALGRKEVVATLLPSAKEQQKEEEEKRLKEHLTES
LFFEQSFQPENRAVSSGPSLLTVFLGVVCIAALMLPTLGDVESLVPLYLHLSVNQKLVAAYILGLITMAI
LRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019556
ORF Size 639 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019556.3
RefSeq Size 2358 bp
RefSeq ORF 642 bp
Locus ID 56180
UniProt ID Q9UJG1
Cytogenetics Xq26.3
Domains MSP_domain
Protein Families Transmembrane
MW 24.1 kDa
Summary Plays a role in differentiation and/or proliferation of mesenchymal stem cells. Proposed to be involved in epithelial-to-mesenchymal transition (EMT). However, another study suggests that it is not required for EMT or stem cell self-renewal and acts during later stages of differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019556" in other vectors (5)
SKU Description Size Price
RC201517L3 Lenti ORF clone of Human motile sperm domain containing 1 (MOSPD1), Myc-DDK-tagged 10 ug
$600.00
RC201517L4 Lenti ORF clone of Human motile sperm domain containing 1 (MOSPD1), mGFP tagged 10 ug
$600.00
RG201517 MOSPD1 (tGFP-tagged) - Human motile sperm domain containing 1 (MOSPD1) 10 ug
$489.00 $500.00
SC113258 MOSPD1 (untagged)-Human motile sperm domain containing 1 (MOSPD1) 10 ug
$300.00
SC323790 MOSPD1 (untagged)-Human motile sperm domain containing 1 (MOSPD1) 10 ug
$300.00

Plasmid Map for RC201517

Download sequence for

Cloning scheme for RC201517

Circular map for RC201517

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