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ALG6 (NM_013339) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC201511
ALG6 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG6)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $472.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ALG6
Synonyms CDG1C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201511 representing NM_013339
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAATGGTACTTGATGACAGTAGTGGTTTTAATAGGACTAACAGTACGATGGACAGTGTCTCTTA
ATTCTTATTCAGGTGCTGGTAAACCGCCTATGTTTGGTGATTATGAAGCTCAGAGACACTGGCAAGAAAT
AACTTTTAATTTACCGGTCAAACAATGGTATTTTAACAGCAGTGATAACAATTTACAGTATTGGGGATTG
GATTACCCACCTCTTACAGCTTATCATAGTCTCCTATGTGCATATGTGGCAAAGTTTATAAATCCAGACT
GGATTGCTCTCCATACATCACGTGGATATGAGAGTCAGGCACATAAGCTCTTCATGCGTACAACAGTTTT
AATTGCTGATCTGCTGATTTACATACCTGCAGTGGTTTTGTACTGTTGTTGCTTAAAAGAAATCTCAACT
AAGAAAAAGATTGCTAATGCATTATGCATCTTGCTGTATCCAGGCCTTATTCTTATAGACTATGGACATT
TTCAATATAATTCTGTGAGTCTTGGCTTTGCTTTGTGGGGTGTTCTTGGAATATCTTGTGACTGCGACCT
CCTAGGGTCACTGGCATTTTGCTTAGCTATAAATTATAAACAGATGGAACTTTACCACGCCTTGCCATTT
TTTTGCTTTTTACTTGGCAAGTGTTTTAAAAAAGGCCTCAAAGGAAAGGGGTTTGTGTTGCTAGTTAAGC
TAGCTTGTATTGTTGTGGCTTCCTTCGTTCTCTGCTGGCTGCCATTCTTTACAGAAAGGGAACAAACCCT
GCAGGTTCTAAGAAGACTCTTCCCGGTTGATCGTGGATTATTTGAGGATAAAGTAGCCAATATTTGGTGC
AGCTTCAATGTCTTTCTGAAGATTAAGGATATTTTGCCACGTCACATCCAATTAATAATGAGCTTTTGTT
CTACGTTTTTGAGCCTGCTTCCTGCATGCATAAAATTAATACTTCAGCCCTCTTCCAAAGGATTCAAATT
TACACTGGTTAGCTGTGCGCTATCATTCTTTTTATTTTCTTTCCAAGTACATGAAAAATCCATTCTCTTG
GTGTCACTACCAGTCTGCTTAGTTTTAAGTGAAATTCCTTTTATGTCTACTTGGTTTTTACTTGTGTCAA
CATTTAGTATGCTACCTCTTCTATTGAAGGATGAACTCCTAATGCCCTCTGTTGTGACAACAATGGCATT
TTTTATAGCTTGTGTAACTTCCTTTTCAATATTTGAAAAGACTTCTGAAGAAGAACTGCAGTTGAAATCC
TTTTCCATTTCTGTGAGGAAATATCTTCCATGTTTTACATTTCTTTCCAGAATTATACAATATTTGTTTC
TTATCTCAGTCATCACTATGGTGCTTCTGACGTTGATGACTGTCACACTGGATCCTCCTCAGAAACTACC
GGACTTGTTTTCTGTATTGGTGTGTTTTGTATCTTGCTTGAACTTCCTGTTCTTCTTGGTATACTTTAAC
ATTATTATTATGTGGGATTCCAAAAGTGGAAGAAATCAGAAGAAAATCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201511 representing NM_013339
Red=Cloning site Green=Tags(s)

MEKWYLMTVVVLIGLTVRWTVSLNSYSGAGKPPMFGDYEAQRHWQEITFNLPVKQWYFNSSDNNLQYWGL
DYPPLTAYHSLLCAYVAKFINPDWIALHTSRGYESQAHKLFMRTTVLIADLLIYIPAVVLYCCCLKEIST
KKKIANALCILLYPGLILIDYGHFQYNSVSLGFALWGVLGISCDCDLLGSLAFCLAINYKQMELYHALPF
FCFLLGKCFKKGLKGKGFVLLVKLACIVVASFVLCWLPFFTEREQTLQVLRRLFPVDRGLFEDKVANIWC
SFNVFLKIKDILPRHIQLIMSFCSTFLSLLPACIKLILQPSSKGFKFTLVSCALSFFLFSFQVHEKSILL
VSLPVCLVLSEIPFMSTWFLLVSTFSMLPLLLKDELLMPSVVTTMAFFIACVTSFSIFEKTSEEELQLKS
FSISVRKYLPCFTFLSRIIQYLFLISVITMVLLTLMTVTLDPPQKLPDLFSVLVCFVSCLNFLFFLVYFN
IIIMWDSKSGRNQKKIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013339
ORF Size 1521 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013339.4
RefSeq Size 3371 bp
RefSeq ORF 1524 bp
Locus ID 29929
UniProt ID Q9Y672
Cytogenetics 1p31.3
Domains Alg6_Alg8
Protein Families Druggable Genome, Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
MW 58.6 kDa
Summary This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the first glucose residue to the growing lipid-linked oligosaccharide precursor of N-linked glycosylation. Mutations in this gene are associated with congenital disorders of glycosylation type Ic. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_013339" in other vectors (4)
SKU Description Size Price
RC201511L3 Lenti-ORF clone of ALG6 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG6) 10 ug
$772.00
RC201511L4 Lenti-ORF clone of ALG6 (mGFP-tagged)-Human asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG6) 10 ug
$772.00
RG201511 ALG6 (tGFP-tagged) - Human asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG6) 10 ug
$672.00
SC115292 ALG6 (untagged)-Human asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG6) 10 ug
$473.00

Plasmid Map for RC201511

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201511
Circular map for RC201511

file-alt Citations

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