TRMT61B (NM_017910) Human Tagged ORF Clone
SKU
RC201499
TRMT61B (Myc-DDK-tagged)-Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B)
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TRMT61B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_017910 |
| ORF Size | 1431 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017910.2, NP_060380.2 |
| RefSeq Size | 1856 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 55006 |
| UniProt ID | Q9BVS5 |
| Cytogenetics | 2p23.2 |
| Protein Families | Druggable Genome |
| MW | 53 kDa |
| Summary | Methyltransferase that catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC) (PubMed:23097428). Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function (PubMed:27631568). In addition to tRNA N(1)-methyltransferase activity, also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA, leading to interfere with mitochondrial translation (PubMed:29107537).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_017910" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201499L3 | Lenti ORF clone of Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC201499L4 | Lenti ORF clone of Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B), mGFP tagged | 10 ug |
$757.00
|
|
| RG201499 | TRMT61B (tGFP-tagged) - Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B) | 10 ug |
$489.00
$657.00
|
|
| SC321424 | TRMT61B (untagged)-Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B) | 10 ug |
$457.00
|
|
Citations
Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
Fang, Babich, Stanton et al
Nucleic Acids Res (2026) 54 (8) DOI: 10.1093/nar/gkag365
Fang, Babich, Stanton et al
Nucleic Acids Res (2026) 54 (8) DOI: 10.1093/nar/gkag365
Cited by 3
AI Summary: OriGene's TRMT61B plasmid (RC201499) was used for FLAG-tagged TRMT61B overexpression in 293-F cells, enabling the study of TRMT61B methyltransferase activity and its effects on mRNA translation in this paper.
Snippet: h 8% CO2.FLAG-tagged TRMT61B expression and purification293-F cells were transfected with 293fectin (Invitrogen, 12347019) and TRMT61B plasmid (Origene, RC201499) following manufacturer instructions. In short, cells were diluted to 1 million/ml in fresh media, for a total volume of 1 l cells. One-thousand micrograms DNA and 2 ml 293fectin wer
Investigating the Regulation of N1-Methyladenosine in Human mRNA
Fang
Thesis (2026)
Fang
Thesis (2026)
Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
Fang, Babich, Stanton et al
bioRxiv (2025)
Fang, Babich, Stanton et al
bioRxiv (2025)
AI Summary: OriGene contributed Myc-DDK-TRMT61B overexpression plasmid (RC201499) and a negative control plasmid (MR227353), which were essential for studying TRMT61B methyltransferase activity and its effects on mRNA translation in this paper.
Snippet: Myc-DDK-TRMT61B overexpression plasmid and negative control plasmid were obtained from Origene (RC201499, MR227353). … FLAG-tagged TRMT61B expression and purification 293-F cells were transfected with 293fectin (Invitrogen, 12347019) and TRMT61B plasmid (Origene RC201499) following manufacturer instructions. …
Showing 1-3 of 3 papers.
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