ERGIC2 (NM_016570) Human Tagged ORF Clone

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SKU
RC201479
ERGIC2 (Myc-DDK-tagged)-Human ERGIC and golgi 2 (ERGIC2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ERGIC2
Synonyms cd002; CDA14; Erv41; PTX1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201479 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCGACTGAATCGGAAAAAAACTTTAAGTTTGGTAAAAGAGTTGGATGCCTTTCCGAAGGTTCCTG
AGAGCTATGTAGAGACTTCAGCCAGTGGAGGTACAGTTTCTCTAATAGCATTTACAACTATGGCTTTATT
AACCATAATGGAATTCTCAGTATATCAAGATACATGGATGAAGTATGAATACGAAGTAGACAAGGATTTT
TCTAGCAAATTAAGAATTAATATAGATATTACTGTTGCCATGAAGTGTCAATATGTTGGAGCGGATGTAT
TGGATTTAGCAGAAACAATGGTTGCATCTGCAGATGGTTTAGTTTATGAACCAACAGTATTTGATCTTTC
ACCACAGCAGAAAGAGTGGCAGAGGATGCTGCAGCTGATTCAGAGTAGGCTACAAGAAGAGCATTCACTT
CAAGATGTGATATTTAAAAGTGCTTTTAAAAGTACATCAACAGCTCTTCCACCAAGAGAAGATGATTCAT
CACAGTCTCCAAATGCATGCAGAATTCATGGCCATCTATATGTCAATAAAGTAGCAGGGAATTTTCACAT
AACAGTGGGCAAGGCAATTCCACATCCTCGTGGTCATGCACATTTGGCAGCACTTGTCAACCATGAATCT
TACAATTTTTCTCATAGAATAGATCATTTGTCTTTTGGAGAGCTTGTTCCAGCAATTATTAATCCTTTAG
ATGGAACTGAAAAAATTGCTATAGATCACAACCAGATGTTCCAATATTTTATTACAGTTGTGCCAACAAA
ACTACATACATATAAAATATCAGCAGACACCCATCAGTTTTCTGTGACAGAAAGGGAACGTATCATTAAC
CATGCTGCAGGCAGCCATGGAGTCTCTGGGATATTTATGAAATATGATCTCAGTTCTCTTATGGTGACAG
TTACTGAGGAGCACATGCCATTCTGGCAGTTTTTTGTAAGACTCTGTGGTATTGTTGGAGGAATCTTTTC
AACAACAGGCATGTTACATGGAATTGGAAAATTTATAGTTGAAATAATTTGCTGTCGTTTCAGACTTGGA
TCCTATAAACCTGTCAATTCTGTTCCTTTTGAGGATGGCCACACAGACAACCACTTACCTCTTTTAGAAA
ATAATACACAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201479 protein sequence
Red=Cloning site Green=Tags(s)

MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDF
SSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSL
QDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHES
YNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIIN
HAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLG
SYKPVNSVPFEDGHTDNHLPLLENNTH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016570
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016570.3
RefSeq Size 1781 bp
RefSeq ORF 1134 bp
Locus ID 51290
UniProt ID Q96RQ1, A0A024RB08, Q86TD3
Cytogenetics 12p11.22
MW 42.5 kDa
Summary ERGIC2, or PTX1, is a ubiquitously expressed nuclear protein that is downregulated in prostate carcinoma (Kwok et al., 2001 PubMed 11445006).supplied by OMIM, Aug 2008
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Documents
Resources
"NM_016570" in other vectors (4)
SKU Description Size Price
RC201479L3 Lenti ORF clone of Human ERGIC and golgi 2 (ERGIC2), Myc-DDK-tagged 10 ug
$757.00
RC201479L4 Lenti ORF clone of Human ERGIC and golgi 2 (ERGIC2), mGFP tagged 10 ug
$757.00
RG201479 ERGIC2 (tGFP-tagged) - Human ERGIC and golgi 2 (ERGIC2) 10 ug
$489.00 $657.00
SC108733 ERGIC2 (untagged)-Human ERGIC and golgi 2 (ERGIC2) 10 ug
$457.00

Plasmid Map for RC201479

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Cloning scheme for RC201479

Circular map for RC201479

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