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FASTKD2 (NM_014929) Human Tagged ORF Clone

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SKU
RC201476
FASTKD2 (Myc-DDK-tagged)-Human FAST kinase domains 2 (FASTKD2), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $650.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FASTKD2
Synonyms COXPD44; KIAA0971
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201476 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGACAACTTTGAAGCCATTTGGAAGTGTTTCAGTGGAGAGCAAAATGAATAACAAAGCGGGCTCCT
TTTTCTGGAACCTTAGACAATTCAGTACATTAGTTTCAACAAGCAGAACTATGAGGCTATGTTGTTTGGG
ACTTTGCAAACCAAAAATAGTTCATTCAAACTGGAACATTTTAAATAACTTTCATAACAGAATGCAATCA
ACTGATATCATTAGATATCTCTTTCAGGATGCATTCATTTTTAAATCAGATGTTGGCTTTCAAACAAAGG
GCATAAGCACTCTAACAGCCCTTAGAATTGAAAGACTACTTTATGCTAAAAGACTGTTTTTTGACTCAAA
GCAGTCTCTTGTCCCTGTTGATAAATCTGATGATGAATTGAAGAAAGTAAACCTTAATCATGAAGTCTCC
AATGAAGATGTTCTTACCAAGGAAACAAAACCAAACCGTATCAGCAGTAGAAAACTGTCTGAGGAATGTA
ATTCCCTGAGTGATGTGTTAGATGCATTTTCAAAAGCGCCCACATTTCCTAGTAGCAACTATTTCACAGC
AATGTGGACAATTGCCAAAAGACTGTCTGATGACCAGAAGCGCTTTGAAAAACGACTGATGTTTAGCCAC
CCTGCATTTAATCAGCTCTGTGAACATATGATGAGAGAAGCCAAGATCATGCAGTATAAGTACCTACTGT
TCAGTCTTCACGCCATAGTGAAGCTTGGAATCCCTCAGAACACTATTTTGGTGCAGACTTTGCTGAGGGT
GACCCAGGAACGTATCAATGAGTGTGATGAGATATGCCTTTCAGTTTTGTCAACTGTTTTAGAGGCAATG
GAACCATGCAAGAATGTTCATGTTCTACGAACGGGATTCAGAATACTAGTTGATCAGCAAGTTTGGAAAA
TAGAAGATGTCTTCACATTACAAGTTGTGATGAAGTGTATTGGAAAAGATGCACCGATTGCTCTTAAGAG
GAAACTGGAGATGAAAGCCTTGAGGGAATTAGACAGATTTTCTGTTTTGAATAGCCAACACATGTTTGAA
GTACTAGCTGCCATGAATCACCGATCTCTTATACTCCTGGATGAATGCAGTAAGGTGGTCCTAGATAATA
TCCATGGGTGTCCTTTAAGAATAATGATCAACATATTGCAGTCCTGCAAAGACCTCCAGTACCATAATTT
GGATCTCTTCAAGGGACTTGCAGATTATGTGGCTGCAACTTTCGACATCTGGAAGTTCAGAAAAGTTCTT
TTTATCCTCATTTTATTTGAAAACCTTGGCTTTCGACCTGTTGGTTTAATGGACCTGTTTATGAAGAGAA
TAGTAGAGGATCCTGAATCCCTAAACATGAAAAACATTCTATCTATTCTTCATACTTACTCTTCTCTCAA
TCATGTCTACAAATGCCAGAACAAAGAACAGTTCGTGGAAGTTATGGCTAGTGCTCTGACTGGTTATCTT
CACACTATTTCTTCTGAAAACTTATTGGATGCAGTATATTCATTTTGCTTGATGAATTACTTTCCCCTGG
CTCCTTTTAATCAGCTTCTGCAAAAAGACATCATCAGTGAGCTGCTGACATCAGATGACATGAAGAATGC
TTACAAGCTGCATACTTTGGATACTTGTCTAAAACTTGATGATACTGTCTATCTGAGGGACATAGCCTTG
TCACTCCCACAGCTGCCGCGGGAGCTGCCATCGTCACATACAAATGCAAAGGTGGCAGAGGTGCTGAGCA
GCCTTCTGGGAGGTGAAGGACACTTCTCAAAGGATGTGCACTTGCCACACAATTATCATATTGATTTTGA
AATCAGAATGGACACTAACAGGAATCAAGTGCTACCACTTTCTGATGTGGATACAACTTCTGCTACAGAT
ATTCAAAGAGTAGCTGTGCTATGTGTTTCCAGATCTGCTTATTGTTTGGGTTCAAGCCACCCCAGAGGAT
TCCTTGCTATGAAAATGCGGCATTTGAATGCAATGGGTTTTCATGTGATCTTGGTCAATAACTGGGAGAT
GGACAAACTAGAGATGGAAGATGCAGTCACATTTTTGAAGACTAAAATCTATTCAGTAGAAGCTCTTCCT
GTTGCTGCTGTAAATGTGCAAAGCACACAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201476 protein sequence
Red=Cloning site Green=Tags(s)

MLTTLKPFGSVSVESKMNNKAGSFFWNLRQFSTLVSTSRTMRLCCLGLCKPKIVHSNWNILNNFHNRMQS
TDIIRYLFQDAFIFKSDVGFQTKGISTLTALRIERLLYAKRLFFDSKQSLVPVDKSDDELKKVNLNHEVS
NEDVLTKETKPNRISSRKLSEECNSLSDVLDAFSKAPTFPSSNYFTAMWTIAKRLSDDQKRFEKRLMFSH
PAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQERINECDEICLSVLSTVLEAM
EPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPIALKRKLEMKALRELDRFSVLNSQHMFE
VLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVL
FILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYL
HTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIAL
SLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATD
IQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALP
VAAVNVQSTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014929
ORF Size 2130 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014929.3
RefSeq Size 6837 bp
RefSeq ORF 2133 bp
Locus ID 22868
UniProt ID Q9NYY8, A0A024R3X5
Cytogenetics 2q33.3
MW 81.5 kDa
Summary This gene encodes a protein that is localized in the mitochondrial inner compartment and that may play a role in mitochondrial apoptosis. Nonsense mutations have been reported to result in cytochrome c oxidase deficiency. provided by RefSeq, Oct 2008
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"NM_014929" in other vectors (5)
SKU Description Size Price
RC201476L3 Lenti ORF clone of Human FAST kinase domains 2 (FASTKD2), transcript variant 3, Myc-DDK-tagged 10 ug
$950.00
RC201476L4 Lenti ORF clone of Human FAST kinase domains 2 (FASTKD2), transcript variant 3, mGFP tagged 10 ug
$950.00
RG201476 FASTKD2 (tGFP-tagged) - Human FAST kinase domains 2 (FASTKD2), transcript variant 3 10 ug
$789.00 $850.00
SC114768 FASTKD2 (untagged)-Human FAST kinase domains 2 (FASTKD2), transcript variant 3 10 ug
$651.00
SC324159 FASTKD2 (untagged)-Human FAST kinase domains 2 (FASTKD2), transcript variant 3 10 ug
$651.00

Plasmid Map for RC201476

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201476
Circular map for RC201476

file-alt Citations

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