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LACTB2 (NM_016027) Human Tagged ORF Clone

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SKU
RC201465
LACTB2 (Myc-DDK-tagged)-Human lactamase, beta 2 (LACTB2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LACTB2
Synonyms CGI-83
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201465 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGTACTGCAGCGCGTCGAGCGGCTGTCCAATCGAGTCGTGCGTGTGTTGGGCTGTAACCCGG
GTCCCATGACCCTCCAAGGCACCAACACCTACCTAGTGGGGACCGGCCCCAGGAGAATCCTCATTGACAC
TGGAGAACCAGCAATTCCAGAATACATCAGCTGTTTAAAGCAGGCTCTAACTGAATTTAACACAGCAATC
CAGGAAATTGTAGTGACTCACTGGCACCGAGATCATTCTGGAGGCATAGGAGATATTTGTAAAAGCATCA
ATAATGACACTACCTATTGCATTAAAAAACTCCCACGGAATCCTCAGAGAGAAGAAATTATAGGAAATGG
AGAGCAACAATATGTTTATCTGAAAGATGGAGATGTGATTAAGACTGAGGGAGCCACTCTAAGAGTTCTA
TATACCCCTGGCCACACTGATGATCACATGGCTCTACTCTTAGAAGAGGAAAATGCTATCTTTTCTGGAG
ATTGCATCCTAGGGGAAGGAACAACGGTATTTGAAGACCTCTATGATTATATGAACTCTTTAAAAGAGTT
ATTGAAAATCAAAGCTGATATTATATATCCAGGACATGGCCCAGTAATTCATAATGCTGAAGCTAAAATT
CAACAATACATTTCTCACAGAAATATTCGAGAGCAGCAAATTCTTACATTATTTCGTGAGAACTTTGAGA
AATCATTTACAGTAATGGAGCTTGTAAAAATTATTTACAAGAATACTCCTGAGAATTTACATGAAATGGC
TAAACATAATCTCTTACTTCATTTGAAAAAACTAGAAAAAGAAGGAAAAATATTTAGCAACACAGATCCT
GACAAGAAATGGAAAGCTCATCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201465 protein sequence
Red=Cloning site Green=Tags(s)

MAAVLQRVERLSNRVVRVLGCNPGPMTLQGTNTYLVGTGPRRILIDTGEPAIPEYISCLKQALTEFNTAI
QEIVVTHWHRDHSGGIGDICKSINNDTTYCIKKLPRNPQREEIIGNGEQQYVYLKDGDVIKTEGATLRVL
YTPGHTDDHMALLLEEENAIFSGDCILGEGTTVFEDLYDYMNSLKELLKIKADIIYPGHGPVIHNAEAKI
QQYISHRNIREQQILTLFRENFEKSFTVMELVKIIYKNTPENLHEMAKHNLLLHLKKLEKEGKIFSNTDP
DKKWKAHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016027
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016027.3
RefSeq Size 1553 bp
RefSeq ORF 867 bp
Locus ID 51110
UniProt ID Q53H82, A0A024R811
Cytogenetics 8q13.3
MW 32.8 kDa
Summary Endoribonuclease; cleaves preferentially 3' to purine-pyrimidine dinucleotide motifs in single-stranded RNA. The cleavage product contains a free 3' -OH group. Has no activity with double-stranded RNA or DNA. Required for normal mitochondrial function and cell viability.UniProtKB/Swiss-Prot Function
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"NM_016027" in other vectors (4)
SKU Description Size Price
RC201465L3 Lenti ORF clone of Human lactamase, beta 2 (LACTB2), Myc-DDK-tagged 10 ug
$600.00
RC201465L4 Lenti ORF clone of Human lactamase, beta 2 (LACTB2), mGFP tagged 10 ug
$600.00
RG201465 LACTB2 (tGFP-tagged) - Human lactamase, beta 2 (LACTB2) 10 ug
$489.00 $500.00
SC322229 LACTB2 (untagged)-Human lactamase, beta 2 (LACTB2) 10 ug
$300.00

Plasmid Map for RC201465

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201465
Circular map for RC201465

file-alt Citations

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