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VPS37B (NM_024667) Human Tagged ORF Clone

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SKU
RC201432
VPS37B (Myc-DDK-tagged)-Human vacuolar protein sorting 37 homolog B (S. cerevisiae) (VPS37B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VPS37B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201432 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCGCCGGGAGCGAAGCCCGGTTCGCCGGGCTGTCGCTGGTGCAGCTCAACGAGCTGCTGGAGG
ACGAGGGCCAGCTGACGGAGATGGTGCAGAAGATGGAGGAGACACAGAATGTTCAGCTTAACAAAGAAAT
GACACTTGCCAGCAACCGGAGCCTGGCAGAAGGAAACCTTTTGTACCAGCCCCAGCTGGACACGTTGAAA
GCACGCTTGACCCAGAAATACCAGGAACTCCAGGTTCTCTTTGAAGCCTATCAGATAAAGAAGACCAAAT
TAGACAGACAGTCTAGCAGTGCTTCCTTGGAGACCCTGTTAGCACTTCTTCAGGCAGAAGGGGCCAAGAT
TGAGGAAGACACTGAGAACATGGCAGAGAAGTTTCTGGATGGAGAACTTCCTCTGGATTCCTTCATTGAT
GTCTATCAGAGCAAACGGAAACTGGCCCACATGCGACGGGTGAAAATCGAGAAGCTCCAGGAGATGGTCC
TAAAGGGGCAGAGACTCCCACAGGCCCTGGCCCCGCTGCCCCCCAGGCTGCCCGAACTGGCACCTACCGC
CCCCCTTCCCTACCCTGCCCCAGAGGCCAGTGGGCCTCCTGCCGTTGCACCTCGGCGCATCCCCCCCCCA
CCACCCCCGGTGCCTGCGGGACGCTTAGCCACCCCGTTTACTGCGGCCATGAGTTCGGGACAGGCCGTGC
CGTACCCAGGATTACAGTGCCCGCCCCTGCCCCCCCGCGTGGGCCTCCCCACTCAGCAAGGATTCTCTTC
GCAGTTCGTGTCCCCATATCCGCCACCTCTCCCTCAGAGACCCCCGCCCCGGCTCCCTCCACACCAGCCG
GGTTTCATCCTCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201432 protein sequence
Red=Cloning site Green=Tags(s)

MAGAGSEARFAGLSLVQLNELLEDEGQLTEMVQKMEETQNVQLNKEMTLASNRSLAEGNLLYQPQLDTLK
ARLTQKYQELQVLFEAYQIKKTKLDRQSSSASLETLLALLQAEGAKIEEDTENMAEKFLDGELPLDSFID
VYQSKRKLAHMRRVKIEKLQEMVLKGQRLPQALAPLPPRLPELAPTAPLPYPAPEASGPPAVAPRRIPPP
PPPVPAGRLATPFTAAMSSGQAVPYPGLQCPPLPPRVGLPTQQGFSSQFVSPYPPPLPQRPPPRLPPHQP
GFILQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024667
ORF Size 855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024667.3
RefSeq Size 2766 bp
RefSeq ORF 858 bp
Locus ID 79720
UniProt ID Q9H9H4, A0A024RBU0
Cytogenetics 12q24.31
Protein Pathways Endocytosis
MW 31.3 kDa
Summary Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.UniProtKB/Swiss-Prot Function
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"NM_024667" in other vectors (6)
SKU Description Size Price
RC201432L1 Lenti ORF clone of Human vacuolar protein sorting 37 homolog B (S. cerevisiae) (VPS37B), Myc-DDK-tagged 10 ug
$600.00
RC201432L2 Lenti ORF clone of Human vacuolar protein sorting 37 homolog B (S. cerevisiae) (VPS37B), mGFP tagged 10 ug
$600.00
RC201432L3 Lenti ORF clone of Human vacuolar protein sorting 37 homolog B (S. cerevisiae) (VPS37B), Myc-DDK-tagged 10 ug
$600.00
RC201432L4 Lenti ORF clone of Human vacuolar protein sorting 37 homolog B (S. cerevisiae) (VPS37B), mGFP tagged 10 ug
$600.00
RG201432 VPS37B (tGFP-tagged) - Human vacuolar protein sorting 37 homolog B (S. cerevisiae) (VPS37B) 10 ug
$500.00
SC320163 VPS37B (untagged)-Human vacuolar protein sorting 37 homolog B (S. cerevisiae) (VPS37B) 10 ug
$300.00

Plasmid Map for RC201432

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201432
Circular map for RC201432

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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