SGTA (NM_003021) Human Tagged ORF Clone

SKU
RC201429
SGTA (Myc-DDK-tagged)-Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA)
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$300.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SGTA
Synonyms alphaSGT; hSGT; SGT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201429 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAACAAGAAGCGCCTGGCCTACGCCATCATCCAGTTCCTGCATGACCAGCTCCGGCACGGGGGCC
TCTCGTCCGATGCTCAGGAGAGCTTGGAAGTCGCCATCCAGTGCCTGGAGACTGCGTTTGGGGTGACGGT
AGAAGACAGTGACCTTGCGCTCCCTCAGACTCTGCCGGAGATATTTGAAGCGGCTGCCACGGGCAAGGAG
ATGCCGCAGGACCTGAGGAGCCCCGCGCGAACCCCGCCTTCCGAGGAGGACTCAGCAGAGGCAGAGCGCC
TCAAAACCGAAGGAAACGAGCAGATGAAAGTGGAAAACTTTGAAGCTGCCGTGCATTTCTACGGAAAAGC
CATCGAGCTCAACCCAGCCAACGCCGTCTATTTCTGCAACAGAGCCGCAGCCTACAGCAAACTCGGCAAC
TACGCAGGCGCGGTGCAGGACTGTGAGCGGGCCATCTGCATTGACCCGGCCTACAGCAAGGCCTACGGCA
GGATGGGCCTGGCGCTCTCCAGCCTCAACAAGCACGTGGAGGCCGTGGCTTACTACAAGAAGGCTCTGGA
GCTGGACCCCGACAACGAGACATACAAGTCCAACCTCAAGATAGCGGAGCTGAAGCTGCGGGAGGCCCCC
AGCCCCACGGGAGGCGTGGGCAGCTTCGACATCGCCGGCCTGCTGAACAACCCTGGCTTCATGAGCATGG
CTTCGAACCTAATGAACAATCCCCAGATTCAGCAGCTCATGTCCGGCATGATTTCGGGTGGCAACAACCC
CTTGGGAACTCCCGGCACCAGCCCCTCGCAGAACGACCTGGCCAGCCTCATCCAGGCGGGCCAGCAGTTT
GCCCAGCAGATGCAGCAGCAGAACCCAGAGTTGATAGAGCAGCTCAGGAGCCAGATCCGGAGTCGGACGC
CCAGCGCCAGCAACGACGACCAGCAGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201429 protein sequence
Red=Cloning site Green=Tags(s)

MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKE
MPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGN
YAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAP
SPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQF
AQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_003021
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003021.4
RefSeq Size 2358 bp
RefSeq ORF 942 bp
Locus ID 6449
UniProt ID O43765
Cytogenetics 19p13.3
Domains TPR
Protein Families Druggable Genome
MW 34.1 kDa
Summary This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC201429L1 Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), Myc-DDK-tagged 10 ug
$600.00
RC201429L2 Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), mGFP tagged 10 ug
$600.00
RC201429L3 Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), Myc-DDK-tagged 10 ug
$600.00
RC201429L4 Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), mGFP tagged 10 ug
$600.00
RG201429 SGTA (tGFP-tagged) - Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA) 10 ug
$489.00 MSRP $500.00 MSRP $500.00
SC118251 SGTA (untagged)-Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA) 10 ug
$300.00

Citations

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