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PWP2H (PWP2) (NM_005049) Human Tagged ORF Clone

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SKU
RC201424
PWP2 (Myc-DDK-tagged)-Human PWP2 periodic tryptophan protein homolog (yeast) (PWP2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $841.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PWP2H
Synonyms EHOC-17; PWP2H; UTP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201424 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTCGCTTACCGGTTTTCAAATTTGCTGGGTACGGTGTACCGGCGTGGGAACCTAAATTTTACCT
GCAATGGAAATTCAGTTATCAGTCCCGTGGGCAATAGAGTCACTGTATTTGACCTTAAAAACAACAAATC
TGACACGCTGCCCCTGGCCACTCGGTACAACGTCAAGTGCGTGGGGCTGTCCCCGGATGGCCGCCTCGCT
ATCATCGTCGATGAAGGGGGCGATGCGCTGCTGGTCAGCCTGGTCTGCAGGTCTGTGCTGCACCACTTCC
ACTTCAAGGGCTCTGTGCACAGTGTGTCCTTCTCCCCTGATGGCAGGAAGTTTGTTGTCACAAAGGGTAA
CATTGCCCAGATGTATCATGCCCCTGGGAAGAAGCGGGAGTTCAACGCCTTCGTTCTGGACAAGACCTAT
TTTGGGCCCTACGATGAGACCACCTGCATCGACTGGACGGATGACTCCAGGTGCTTTGTGGTTGGGAGCA
AAGACATGTCCACCTGGGTGTTCGGAGCCGAGCGCTGGGACAACCTCATCTACTATGCACTGGGGGGACA
TAAGGATGCCATCGTGGCCTGCTTCTTTGAATCCAACAGCCTGGACCTGTACTCACTCAGCCAGGACGGA
GTGCTGTGCATGTGGCAGTGTGACACGCCCCCCGAGGGCTTGCGGCTGAAGCCCCCTGCGGGCTGGAAAG
CAGACCTGTTGCAGCGGGAGGAGGAAGAGGAGGAGGAGGAGGACCAGGAGGGCGACAGAGAGACCACCAT
CCGGGGAAAAGCCACTCCGGCCGAGGAGGAGAAGACAGGAAAAGTGAAGTACTCACGGCTGGCCAAGTAC
TTCTTCAATAAAGAAGGGGATTTTAACAACCTGACAGCTGCAGCATTTCATAAGAAGTCTCACCTCTTGG
TCACTGGCTTTGCTTCTGGAATCTTCCATCTTCATGAGCTGCCAGAGTTTAACCTCATCCACTCCCTGAG
CATCTCAGATCAGAGCATCGCCTCAGTGGCCATCAATAGCTCGGGGGACTGGATTGCTTTTGGCTGTTCA
GGCCTGGGCCAGCTGCTGGTGTGGGAGTGGCAGAGTGAGTCCTACGTGCTCAAGCAGCAGGGCCACTTCA
ACAGCATGGTGGCCCTGGCCTACTCGCCCGACGGACAGTACATCGTGACTGGCGGGGACGACGGCAAGGT
CAAGGTGTGGAACACCCTCAGCGGCTTCTGCTTCGTCACTTTTACGGAGCACTCCAGCGGGGTGACCGGT
GTGACCTTTACTGCCACCGGCTACGTTGTGGTGACCTCATCCATGGACGGGACCGTGCGAGCCTTTGACC
TTCACAGGTACCGAAACTTCCGCACCTTCACCTCTCCACGCCCCACCCAGTTCTCCTGTGTGGCGGTGGA
TGCGAGCGGTGAGATCGTCTCTGCAGGGGCGCAGGACTCCTTTGAGATTTTCGTGTGGTCCATGCAGACA
GGCAGGCTCCTTGATGTTTTGTCTGGACACGAAGGGCCCATCAGTGGTCTGTGTTTTAACCCAATGAAGT
CCGTCCTGGCCAGTGCCTCCTGGGACAAGACGGTGCGCCTATGGGACATGTTTGACAGCTGGAGGACCAA
GGAGACGCTGGCCCTGACCTCTGATGCTCTGGCTGTGACTATTCGCCCTGATGGTGCGGAGCTGGCTGTG
GCCACACTGAACTCACAGATCACCTTCTGGGACCCTGAGAACGCGGTGCAGACGGGCTCCATTGAGGGCA
GGCATGACCTCAAGACTGGCAGGAAGGAGCTGGACAAGATTACAGCCAAGCACGCGGCCAAGGGGAAGGC
CTTCACCGCCCTGTGCTACTCTGCAGACGGCCACAGCATCCTGGCGGGAGGCATGTCCAAGTTCGTGTGC
ATCTACCACGTCCGTGAGCAGATTCTCATGAAGAGGTTCGAGATCTCTTGCAACCTGTCTCTGGACGCCA
TGGAGGAATTTTTGAACCGAAGAAAAATGACAGAGTTTGGCAACCTGGCACTAATTGATCAGGATGCTGG
GCAGGAGGATGGAGTCGCGATACCACTGCCAGGCGTCAGGAAAGGTGACATGAGTTCTCGGCACTTCAAA
CCTGAGATCAGGGTGACCTCACTCCGCTTCTCTCCCACTGGGCGCTGCTGGGCGGCCACCACCACGGAGG
GACTCCTCATCTACTCCCTGGACACCCGCGTGCTCTTTGACCCGTTTGAGCTGGACACCAGCGTCACCCC
CGGGAGGGTGCGCGAGGCACTGCGCCAGCAGGACTTCACCAGGGCCATCCTCATGGCCCTCCGGCTCAAC
GAGAGCAAACTGGTGCAGGAGGCCCTGGAGGCGGTGCCCAGGGGCGAGATTGAAGTGGTCACCTCCTCCC
TTCCTGAACTGTATGTGGAGAAAGTGCTGGAGTTTTTAGCTTCCTCCTTTGAAGTGTCTCGCCACCTGGA
ATTCTACCTCCTCTGGACTCACAAACTGCTCATGTTGCACGGACAGAAGCTGAAGTCCAGAGCCGGGACG
CTGCTGCCTGTCATTCAGTTCCTCCAGAAGAGCATCCAGCGGCACCTGGACGACCTGTCGAAACTCTGTA
GCTGGAACCACTATAACATGCAGTACGCACTAGCAGTTTCCAAGCAGCGGGGCACAAAACGCTCCCTAGA
CCCGCTGGGAAGTGAGGAGGAGGCAGAAGCATCTGAAGATGACAGCCTGCATCTGCTTGGAGGAGGAGGC
AGAGACTCAGAAGAAGAGATGCTGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201424 protein sequence
Red=Cloning site Green=Tags(s)

MKFAYRFSNLLGTVYRRGNLNFTCNGNSVISPVGNRVTVFDLKNNKSDTLPLATRYNVKCVGLSPDGRLA
IIVDEGGDALLVSLVCRSVLHHFHFKGSVHSVSFSPDGRKFVVTKGNIAQMYHAPGKKREFNAFVLDKTY
FGPYDETTCIDWTDDSRCFVVGSKDMSTWVFGAERWDNLIYYALGGHKDAIVACFFESNSLDLYSLSQDG
VLCMWQCDTPPEGLRLKPPAGWKADLLQREEEEEEEEDQEGDRETTIRGKATPAEEEKTGKVKYSRLAKY
FFNKEGDFNNLTAAAFHKKSHLLVTGFASGIFHLHELPEFNLIHSLSISDQSIASVAINSSGDWIAFGCS
GLGQLLVWEWQSESYVLKQQGHFNSMVALAYSPDGQYIVTGGDDGKVKVWNTLSGFCFVTFTEHSSGVTG
VTFTATGYVVVTSSMDGTVRAFDLHRYRNFRTFTSPRPTQFSCVAVDASGEIVSAGAQDSFEIFVWSMQT
GRLLDVLSGHEGPISGLCFNPMKSVLASASWDKTVRLWDMFDSWRTKETLALTSDALAVTIRPDGAELAV
ATLNSQITFWDPENAVQTGSIEGRHDLKTGRKELDKITAKHAAKGKAFTALCYSADGHSILAGGMSKFVC
IYHVREQILMKRFEISCNLSLDAMEEFLNRRKMTEFGNLALIDQDAGQEDGVAIPLPGVRKGDMSSRHFK
PEIRVTSLRFSPTGRCWAATTTEGLLIYSLDTRVLFDPFELDTSVTPGRVREALRQQDFTRAILMALRLN
ESKLVQEALEAVPRGEIEVVTSSLPELYVEKVLEFLASSFEVSRHLEFYLLWTHKLLMLHGQKLKSRAGT
LLPVIQFLQKSIQRHLDDLSKLCSWNHYNMQYALAVSKQRGTKRSLDPLGSEEEAEASEDDSLHLLGGGG
RDSEEEMLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005049
ORF Size 2757 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005049.3
RefSeq Size 3277 bp
RefSeq ORF 2760 bp
Locus ID 5822
UniProt ID Q15269
Cytogenetics 21q22.3
Domains PWP2, WD40
MW 102.4 kDa
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Documents
Resources
"NM_005049" in other vectors (5)
SKU Description Size Price
RC201424L3 Lenti ORF clone of Human PWP2 periodic tryptophan protein homolog (yeast) (PWP2), Myc-DDK-tagged 10 ug
$1,141.00
RC201424L4 Lenti ORF clone of Human PWP2 periodic tryptophan protein homolog (yeast) (PWP2), mGFP tagged 10 ug
$1,141.00
RG201424 PWP2 (tGFP-tagged) - Human PWP2 periodic tryptophan protein homolog (yeast) (PWP2) 10 ug
$789.00 $1,041.00
SC108537 PWP2 (untagged)-Human PWP2 periodic tryptophan protein homolog (yeast) (PWP2) 10 ug
$842.00
SC320349 PWP2 (untagged)-Human PWP2 periodic tryptophan protein homolog (yeast) (PWP2) 10 ug
$842.00

Plasmid Map for RC201424

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201424
Circular map for RC201424

file-alt Citations

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