NPDC1 (NM_015392) Human Tagged ORF Clone

SKU
RC201415
NPDC1 (Myc-DDK-tagged)-Human neural proliferation, differentiation and control, 1 (NPDC1)
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NPDC1
Synonyms CAB; CAB-; CAB-1; CAB1; NPDC-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201415 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACGCCGCTGCCTCCGCCCTCCCCGCGGCACCTGCGGCTGCTGCGGCTGCTGCTCTCCGGCCTCG
TCCTCGGCGCCGCCCTGCGTGGAGCCGCCGCCGGCCACCCGGATGTAGCCGCCTGTCCCGGGAGCCTGGA
CTGTGCCCTGAAGAGGCGGGCAAGGTGTCCTCCTGGTGCACATGCCTGTGGGCCCTGCCTTCAGCCCTTC
CAGGAGGACCAGCAAGGGCTCTGTGTGCCCAGGATGCGCCGGCCTCCAGGCGGGGGCCGGCCCCAGCCCA
GACTGGAAGATGAGATTGACTTCCTGGCCCAGGAGCTTGCCCGGAAGGAGTCTGGACACTCAACTCCGCC
CCTACCCAAGGACCGACAGCGGCTCCCGGAGCCTGCCACCCTGGGCTTCTCGGCACGGGGGCAGGGGCTG
GAGCTGGGCCTCCCCTCCACTCCAGGAACCCCCACGCCCACGCCCCACACCTCCCTGGGCTCCCCTGTGT
CATCCGACCCGGTGCACATGTCGCCCCTGGAGCCCCGGGGAGGGCAAGGCGACGGCCTCGCCCTTGTGCT
GATCCTGGCGTTCTGTGTGGCCGGTGCAGCCGCCCTCTCCGTAGCCTCCCTCTGCTGGTGCAGGCTGCAG
CGTGAGATCCGCCTGACTCAGAAGGCCGACTACGCCACTGCGAAGGCCCCTGGCTCACCTGCAGCTCCCC
GGATCTCGCCTGGGGACCAGCGGCTGGCACAGAGCGCGGAGATGTACCACTACCAGCACCAACGGCAACA
GATGCTGTGCCTGGAGCGGCATAAAGAGCCACCCAAGGAGCTGGACACGGCCTCCTCGGATGAGGAGAAT
GAGGACGGAGACTTCACGGTGTACGAGTGCCCGGGCCTGGCCCCGACCGGGGAAATGGAGGTGCGCAACC
CTCTGTTCGACCACGCCGCACTGTCCGCGCCCCTGCCGGCCCCCAGCTCACCGCCTGCACTGCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201415 protein sequence
Red=Cloning site Green=Tags(s)

MATPLPPPSPRHLRLLRLLLSGLVLGAALRGAAAGHPDVAACPGSLDCALKRRARCPPGAHACGPCLQPF
QEDQQGLCVPRMRRPPGGGRPQPRLEDEIDFLAQELARKESGHSTPPLPKDRQRLPEPATLGFSARGQGL
ELGLPSTPGTPTPTPHTSLGSPVSSDPVHMSPLEPRGGQGDGLALVLILAFCVAGAAALSVASLCWCRLQ
REIRLTQKADYATAKAPGSPAAPRISPGDQRLAQSAEMYHYQHQRQQMLCLERHKEPPKELDTASSDEEN
EDGDFTVYECPGLAPTGEMEVRNPLFDHAALSAPLPAPSSPPALP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_015392
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015392.4
RefSeq Size 1548 bp
RefSeq ORF 978 bp
Locus ID 56654
UniProt ID Q9NQX5
Cytogenetics 9q34.3
Protein Families Transmembrane
MW 34.5 kDa
Summary Suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. Might be involved in transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function]
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RC201415L3 Lenti ORF clone of Human neural proliferation, differentiation and control, 1 (NPDC1), Myc-DDK-tagged 10 ug
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Citations

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